CAJ | 학술논문

目的探讨切口痛大鼠胃体胃动素水平的变化.方法 SPF级健康雄性SD大鼠84只,6～8周龄,体重180 ～ 220 g,采用随机数字表法,将其分为2组(n=42):生理盐水组(NS组)和切口痛组(P组).P组行右足跖肌切口以制备切口病模型.2组随机取6只大鼠,于术前24 h(To)、术后1 h(T1)、6 h(T2)、24 h(T3)、48 h(T4)、72 h(T5)测定机械缩足反应阈(MWT)与热缩足反应潜伏期(TWL),并于上述各时点分别随机取6只大鼠,断头处死,均取胃体粘膜组织,采用ELISA法测定胃动素水平.结果与NS组比较,P组T1～T4时MWT、TWL和胃体胃动素水平降低(P＜0.05),T0、T5时差异无统计学意义(P＞0.05)；与T0时比较,P组MWT、TWL和胃体胃动素水平T1～T4时降低,T5时差异无统计学意义(P＞0.05)；与T1时比较,P组MWT、TWL和胃体胃动素水平T3～T5时升高(P＜0.05)；P组MWT、TWL和胃体胃动素水平T3～T5时逐渐升高(P＜0.05).P组胃体胃动素水平与MWT、TWL均呈正相关性(r分别为0.9597、0.9231,P＜0.01).结论切口痛大鼠胃体胃动素水平明显降低,降低幅度与切口痛程度呈正相关.
목적 탐토절구통대서위체위동소수평적변화.방법 SPF급건강웅성SD대서84지,6～8주령,체중180 ～ 220 g,채용수궤수자표법,장기분위2조(n=42):생리염수조(NS조)화절구통조(P조).P조행우족척기절구이제비절구병모형.2조수궤취6지대서,우술전24 h(To)、술후1 h(T1)、6 h(T2)、24 h(T3)、48 h(T4)、72 h(T5)측정궤계축족반응역(MWT)여열축족반응잠복기(TWL),병우상술각시점분별수궤취6지대서,단두처사,균취위체점막조직,채용ELISA법측정위동소수평.결과 여NS조비교,P조T1～T4시MWT、TWL화위체위동소수평강저(P＜0.05),T0、T5시차이무통계학의의(P＞0.05)；여T0시비교,P조MWT、TWL화위체위동소수평T1～T4시강저,T5시차이무통계학의의(P＞0.05)；여T1시비교,P조MWT、TWL화위체위동소수평T3～T5시승고(P＜0.05)；P조MWT、TWL화위체위동소수평T3～T5시축점승고(P＜0.05).P조위체위동소수평여MWT、TWL균정정상관성(r분별위0.9597、0.9231,P＜0.01).결론 절구통대서위체위동소수평명현강저,강저폭도여절구통정도정정상관.
Objective To investigate the changes in the levels of motilin in the gastric body in a rat model of incisional pain.Methods Eighty-four healthy male Sprague-Dawley rats,weighing 180-220 g,were randomized into 2 groups (n =42 each):normal saline group (NS group) and incisional pain group (P group).In group P an 1 cm long incision was made in the plantar surface of right hindpaw under sevotlurane anesthesia.Six rats were chosen from each group and paw withdrawal threshold to mechanical stimulation (MWT) and paw withdrawal latency to nociceptive thermal stimulation (TWL) were measured at 24 h before operation (To) and 1,6,24,48 and 72 h after operation (T1-5).Six rats were chosen from each group at T0-5 and sacrificed and the gastric mucosal tissues were prepared for measurement of motilin levels by ELISA.Results Compared with group NS,MWT,TWL and motilin levels were significantly decreased at T1-4 (P ＜ 0.05) and no significant change was found at T0 and T5 in P group (P ＞ 0.05).Compared with the baseline value at To,MWT,TWL and motilin levels were significantly decreased at T1-4,and no significant change was found at T5 in group P (P ＞ 0.05).MWT,TWL and motilin levels were significantly higher at T3-T5 than at T1 in group P (P ＜ 0.05).MWT,TWL and motilin levels were increased gradually at T3-T5 in P group (P ＜ 0.05).The motilin levels were positively correlated with MWT and TWL (r =0.9597 and 0.9231,respectively,P ＜ 0.01) in group P.Conclusion The levels of motilin in the gastric body is significantly decreased in a rat model of incisional pain and the decreasing range is positively corre lated with the degree of incisional pain.