CAJ | 학술논문

目的探讨大鼠窒息性心脏停搏复苏(CA-CPR)后心肌微小RNA (miRNA)表达的动态变化.方法清洁级成年雄性SD大鼠64只,体重250 ～ 330 g,采用随机数字表法,将其分为2组(n=32),对照组(C组)和CA-CPR组.采用窒息法建立CA-CPR模型.CA-CPR组于自主循环恢复后3、6、12和24 h(T1-4)时,C组于相应时点取血样,测定血清CK-MB和cTnI浓度,相应时点取心尖部心肌组织,采用Real-Time PCR法检测心肌组织miRNA.以相对于C组心肌miRNA 2倍强度的变化作为CA-CPR组miRNA表达上调或下调的标准.记录大鼠均发生表达变化的miRNA.结果与C组比较,CA-CPR组各时点血清CK-MB和cTnI浓度升高(P＜0.01)；CA-CPR组血清CK-MB浓度T3时达峰值,血清cTnI浓度持续升高(P＜0.05或P＜0.01).自主循环恢复后持续性变化的miRNA有8个,分别为miRNA-1、miRNA-21、miRNA126、miRNA-133a、miRNA-206、miRNA-210、miRNA-320及miRNA-499.miRNA-1各时点表达均上调,T2时最高；miRNA-21各时点表达均下调,T2时最低；miRNA126、miRNA-133a、miRNA-206、miRNA-210和miRNA-499 T1、T2时表达下调,T2时最低,T3、T4时表达上调；miRNA-320T1 ～T3时表达上调,T2时最高,T4时表达下调(P＜0.05或0.01).结论心脏停搏大鼠复苏后有8个miRNA表达发生变化,且变化方式(上调/下调)、趋势不同,但表达变化方式改变的时点相似.
목적 탐토대서질식성심장정박복소(CA-CPR)후심기미소RNA (miRNA)표체적동태변화.방법 청길급성년웅성SD대서64지,체중250 ～ 330 g,채용수궤수자표법,장기분위2조(n=32),대조조(C조)화CA-CPR조.채용질식법건립CA-CPR모형.CA-CPR조우자주순배회복후3、6、12화24 h(T1-4)시,C조우상응시점취혈양,측정혈청CK-MB화cTnI농도,상응시점취심첨부심기조직,채용Real-Time PCR법검측심기조직miRNA.이상대우C조심기miRNA 2배강도적변화작위CA-CPR조miRNA표체상조혹하조적표준.기록대서균발생표체변화적miRNA.결과 여C조비교,CA-CPR조각시점혈청CK-MB화cTnI농도승고(P＜0.01)；CA-CPR조혈청CK-MB농도T3시체봉치,혈청cTnI농도지속승고(P＜0.05혹P＜0.01).자주순배회복후지속성변화적miRNA유8개,분별위miRNA-1、miRNA-21、miRNA126、miRNA-133a、miRNA-206、miRNA-210、miRNA-320급miRNA-499.miRNA-1각시점표체균상조,T2시최고；miRNA-21각시점표체균하조,T2시최저；miRNA126、miRNA-133a、miRNA-206、miRNA-210화miRNA-499 T1、T2시표체하조,T2시최저,T3、T4시표체상조；miRNA-320T1 ～T3시표체상조,T2시최고,T4시표체하조(P＜0.05혹0.01).결론 심장정박대서복소후유8개miRNA표체발생변화,차변화방식(상조/하조)、추세불동,단표체변화방식개변적시점상사.
Objective To investigate the dynamic changes in myocardial microRNA (miRNA) expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest.Methods Sixty-four adult male Sprague-Dawley rats,weighing 250-330 g,were randomly divided into 2 groups (n =32 each):control group (group C) and cardiopulmonary resuscitation after cardiac arrest group (group CA-CPR).The rats were anesthetized with intraperitoneal chloral hydrate 7 ml/kg,tracheostomized and mechanically ventilated.The model of cardiac arrest was established by clamping the endotracheal tube at end expiration and cardiopulmonary resuscitation was performed.At 3,6,12 and 24 h after recovery of spontaneous circulation (T1-4),blood samples were collected for determination of serum creatine-kinase isoenzyme MB (CK-MB) and cardiac troponin I (cTnI) concentrations and myocardial specimens were obtained from the apex for detection of the expression of miRNAs in myocardial tissues (by using RT-PCR).Results Compared with group C,serum CK-MB and cTnI concentrations were significantly increased at each time point in group CA-CPR (P ＜ 0.01).The serum CK-MB concentration reached the peak at T3,and the serum cTnI concentrations continued to increase in group CA-CPR (P ＜ 0.05 or 0.01).There were 8 miRNAs which changed continuously after recovery of spontaneous circulation,and there were miRNA-1,miRNA-21,miRNA126,miRNA-133a,miRNA-206,miRNA-210,miRNA-320 and miRNA-499.The expression of miR-NA-1 was up-regulated at each time point and peaked at T2.The expression of miRNA-21 was down-regulated at each time point and reached the lowest level at T2.The expression of miRNA126,miRNA-133a,miRNA-206,miRNA-210 and miRNA-499 was down-regulated at T1 and T2,was up-regulated at T3 and T4,and reached the lowest level at T2.The expression of miRNA-320 was up-regulated at T1-T3,was down-regulated at T4,and peaked at T2 (P ＜ 0.05 or 0.01).Conclusion Eight miRNAs exhibit changes in expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest and the way and tendency that miRNAs have changed are different,but the time point at which the way that miRNAs have changed is similar.