CAJ | 학술논문

目的评价补体C1q在大鼠肝脏缺血再灌注损伤中的作用.方法健康雄性SD大鼠60只,体重180 ～ 200 g,3～4月龄,采用随机数字表法,将其分为2组:假手术组(S组,n=12),肝脏缺血再灌注组(I/R组,n=48).于再灌注1、3、6、24 h时取肝组织,HE染色后光镜下观察肝组织病理学改变,采用比色法测定肝组织SOD活性和MDA含量,采用荧光实时定量PCR法检测肝组织补体C1q mRNA表达,Western blot法检测肝组织补体C1q表达.结果与S组比较,I/R组随再灌注时间延长肝组织SOD活性逐渐降低,MDA含量逐渐升高,补体C1q及其mRNA表达上调,于再灌注3h时达峰值(P＜0.05).I/R组病理学损伤程度随再灌注时间的延长而加重.结论补体C1q活化参与大鼠肝脏缺血再灌注损伤.
목적 평개보체C1q재대서간장결혈재관주손상중적작용.방법 건강웅성SD대서60지,체중180 ～ 200 g,3～4월령,채용수궤수자표법,장기분위2조:가수술조(S조,n=12),간장결혈재관주조(I/R조,n=48).우재관주1、3、6、24 h시취간조직,HE염색후광경하관찰간조직병이학개변,채용비색법측정간조직SOD활성화MDA함량,채용형광실시정량PCR법검측간조직보체C1q mRNA표체,Western blot법검측간조직보체C1q표체.결과 여S조비교,I/R조수재관주시간연장간조직SOD활성축점강저,MDA함량축점승고,보체C1q급기mRNA표체상조,우재관주3h시체봉치(P＜0.05).I/R조병이학손상정도수재관주시간적연장이가중.결론 보체C1q활화삼여대서간장결혈재관주손상.
Objective To evaluate the role of the complement 1 q (C1 q) in hepatic ischemia-reperfusion (I/R) injury in rats.Methods Sixty healthy male Sprague-Dawley rats,aged 3-4 months,weighing 180-200 g,were randomized into 2 groups:sham operation group (S group,n =12) and hepatic I/R group (I/R group,n =48).Hepatic specimens were obtained at 1,3,6 and 24 h of reperfusion and were then cut and stained with haematoxylin and eosin for examination of histological changes of the liver (with light microscope) and for determination of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content (by colorimetric method),expression of Clq mRNA (using real-time PCR) and expression of Clq (by using Western blot).Results Compared with S group,the activity of SOD was gradually decreased,the content of MDA was gradually increased,and the expression of Clq and Clq mRNA was gradually up-regulated and peaked at 3 h of reperfusion with the prolongation of reperfusion time in I/R group (P ＜ 0.05).The pathological changes of the liver were aggravated with the prolongation of reperfusion time in I/R group.Conclusion Activation of C1 q is involved in hepatic I/R injury in rats.