中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
10期
1223-1225
,共3页
李治松%李艳娜%杜英英%李会新%张卫%阚全程
李治鬆%李豔娜%杜英英%李會新%張衛%闞全程
리치송%리염나%두영영%리회신%장위%감전정
Toll样受体4%白细胞介素1β%肿瘤坏死因子α%RNA,信使%脊髓%疼痛,手术后
Toll樣受體4%白細胞介素1β%腫瘤壞死因子α%RNA,信使%脊髓%疼痛,手術後
Toll양수체4%백세포개소1β%종류배사인자α%RNA,신사%척수%동통,수술후
Toll-like receptor 4%Inter leukin-1beta%Tumor necrosis factor-alpha%RNA,messenger%Spinal cord%Pain,postoperative
目的 切口痛大鼠脊髓Toll样受体4(TLR4)及其下游细胞因子mRNA表达的变化.方法 健康成年雄性SD大鼠58只,体重180 ～ 220 g,建立大鼠切口痛模型.于术前、术后0.5、1、2、8、12h、1、2、3、5和7d时测定术侧与非术侧机械缩足反应阈(MWT);于术前、术后2、8h、1、2、3、5和7d时采用荧光定量PCR法检测脊髓TLR4、IL-1β和TNF-α的mRNA的表达.结果 与术前比较,大鼠术侧术后0.5h～5d MWT降低,术后2、8h、1、2和3d时脊髓TLR4 mRNA、IL-1 mRNA、TNF-α mRNA表达上调(P＜0.05),非术侧MWT差异无统计学意义(P＞0.05);术侧MWT术后2h时最低,之后逐渐升高,脊髓TLR4 mRNA术后1d时表达水平最高,IL-1 mRNA、TNF-α mRNA术后8h时表达水平最高(P＜0.05).TLR4 mRNA表达水平与术侧MWT呈负相关(相关系数-0.484,P＜0.05),IL-1β mRNA和TNF-α mRNA表达水平与TLR4 mRNA表达水平呈正相关(相关系数分别0.294、0.540,P＜0.05).结论 切口痛大鼠脊髓TLR4及下游炎性因子IL-1β、TNF-α的mRNA表达上调,该变化可能参与了切口痛的维持,但并未起主要作用.
目的 切口痛大鼠脊髓Toll樣受體4(TLR4)及其下遊細胞因子mRNA錶達的變化.方法 健康成年雄性SD大鼠58隻,體重180 ～ 220 g,建立大鼠切口痛模型.于術前、術後0.5、1、2、8、12h、1、2、3、5和7d時測定術側與非術側機械縮足反應閾(MWT);于術前、術後2、8h、1、2、3、5和7d時採用熒光定量PCR法檢測脊髓TLR4、IL-1β和TNF-α的mRNA的錶達.結果 與術前比較,大鼠術側術後0.5h～5d MWT降低,術後2、8h、1、2和3d時脊髓TLR4 mRNA、IL-1 mRNA、TNF-α mRNA錶達上調(P＜0.05),非術側MWT差異無統計學意義(P＞0.05);術側MWT術後2h時最低,之後逐漸升高,脊髓TLR4 mRNA術後1d時錶達水平最高,IL-1 mRNA、TNF-α mRNA術後8h時錶達水平最高(P＜0.05).TLR4 mRNA錶達水平與術側MWT呈負相關(相關繫數-0.484,P＜0.05),IL-1β mRNA和TNF-α mRNA錶達水平與TLR4 mRNA錶達水平呈正相關(相關繫數分彆0.294、0.540,P＜0.05).結論 切口痛大鼠脊髓TLR4及下遊炎性因子IL-1β、TNF-α的mRNA錶達上調,該變化可能參與瞭切口痛的維持,但併未起主要作用.
목적 절구통대서척수Toll양수체4(TLR4)급기하유세포인자mRNA표체적변화.방법 건강성년웅성SD대서58지,체중180 ～ 220 g,건립대서절구통모형.우술전、술후0.5、1、2、8、12h、1、2、3、5화7d시측정술측여비술측궤계축족반응역(MWT);우술전、술후2、8h、1、2、3、5화7d시채용형광정량PCR법검측척수TLR4、IL-1β화TNF-α적mRNA적표체.결과 여술전비교,대서술측술후0.5h～5d MWT강저,술후2、8h、1、2화3d시척수TLR4 mRNA、IL-1 mRNA、TNF-α mRNA표체상조(P＜0.05),비술측MWT차이무통계학의의(P＞0.05);술측MWT술후2h시최저,지후축점승고,척수TLR4 mRNA술후1d시표체수평최고,IL-1 mRNA、TNF-α mRNA술후8h시표체수평최고(P＜0.05).TLR4 mRNA표체수평여술측MWT정부상관(상관계수-0.484,P＜0.05),IL-1β mRNA화TNF-α mRNA표체수평여TLR4 mRNA표체수평정정상관(상관계수분별0.294、0.540,P＜0.05).결론 절구통대서척수TLR4급하유염성인자IL-1β、TNF-α적mRNA표체상조,해변화가능삼여료절구통적유지,단병미기주요작용.
Objective To evaluate the changes in the expression of Toll-like receptor 4 (TLR4) mRNA and its down-stream cytokines IL-1β and TNF-α mRNA in spinal cord in a rat model of incisional pain.Methods Fifty-eight male Sprague-Dawley rats,weighing 180-220 g,were used in this study.A 1-cm longitudinal incision was made through skin,fascia and muscle of the plantar aspect of the hindpaw in isoflurane-anesthetized rats.Mechanical paw withdrawal threshold to yon Frey filament stimulation (MWT) on the operated and non-operated sides was measured before operation and at 0.5,1,2,6 and 12 h and 1,2,3,5 and 7 days after operation.Six rats were chosen and sacrificed before operation and at 2 and 8 h and 1,2,3,5 and 7 days after operation.Their lumbar segments (L4-6) of the spinal cord were removed for determination of the expression of TLR4,IL-1β and TNF-α mRNA by real-time PCR.Results Compared with the baseline value before operation,MWT on the operated side was significantly decreased at 0.5 h-5 days after operation,and the expression of TLR4,IL-1β and TNFα mRNA was up-regulated at 2 and 8 h and 1,2 and 3 days after operation (P ＜ 0.05),and no significant change was found in MWT on the non-operated side (P ＞ 0.05).MWT on the operated side was lowest at 2 h after operation and then gradually increased,the expression of TLR4 mRNA peaked on 1 day after operation,and the expression of IL-1 and TNF-α mRNA peaked at 8 h after operation (P ＜ 0.05).The TLR4 mRNA expression was negatively correlated with MWT on the operative side (r =-0.484,P ＜ 0.05),and IL-1 mRNA and TNF-α mRNA expression was positively correlated with TLR4 mRNA (r =0.294 and 0.540,respectively,P ＜ 0.05).Conclusion The expression of TLR4 mRNA and its down-stream cytokines IL-1β and TNF-α(mRNA in spinal cord is up-regulated,this change is involved in the maintenace of incisional pain,but it does not play an important role.
