CAJ | 학술논문

目的评价七氟醚预处理对老龄大鼠术后认知功能障碍的影响.方法健康成年雄性SD大鼠120只,18月龄,体重400 ～ 450 g,采用随机数字表法,将其分为3组(n=40).对照组(C组):仅戊巴比妥钠麻醉,不进行手术;手术组(O组):戊巴比妥钠麻醉后行剖腹探查术,手术时间30 min;七氟醚预处理组(Sev组):吸入2.4％七氟醚30 min,然后吸入空气30 min后处理同O组.于术前30min及术后第1、3、5、7天时,行Morris水迷宫实验,记录逃避潜伏期、平台象限停留时间和穿越原平台次数;并于术前30 min及术后第1、7天时,取海马组织,采用流式细胞术测定海马神经元凋亡率、胞浆Ca2+离子浓度([Ca2+]i);透射电镜下观察海马神经元超微结构.结果与C组比较,O组和Sev组术后逃避潜伏期延长、平台象限停留时间缩短和穿越原平台次数减少,海马神经元凋亡率、胞浆[Ca2+]i升高(P＜0.05);与O组比较,Sev组术后逃避潜伏期缩短、平台象限停留时间延长和穿越原平台次数增多,海马神经元凋亡率、胞浆[Ca2+]i降低(P＜0.05).C组海马神经元胞浆超微结构未见异常,O组海马神经元胞浆超微结构病理学损伤明显,Sev组海马神经元胞浆超微结构病理学损伤减轻.结论 2.4％七氟醚预处理可减轻老龄大鼠术后认知功能障碍,其机制与调节钙稳态失衡,降低海马神经元凋亡有关.
목적 평개칠불미예처리대노령대서술후인지공능장애적영향.방법 건강성년웅성SD대서120지,18월령,체중400 ～ 450 g,채용수궤수자표법,장기분위3조(n=40).대조조(C조):부무파비타납마취,불진행수술;수술조(O조):무파비타납마취후행부복탐사술,수술시간30 min;칠불미예처리조(Sev조):흡입2.4％칠불미30 min,연후흡입공기30 min후처리동O조.우술전30min급술후제1、3、5、7천시,행Morris수미궁실험,기록도피잠복기、평태상한정류시간화천월원평태차수;병우술전30 min급술후제1、7천시,취해마조직,채용류식세포술측정해마신경원조망솔、포장Ca2+리자농도([Ca2+]i);투사전경하관찰해마신경원초미결구.결과 여C조비교,O조화Sev조술후도피잠복기연장、평태상한정류시간축단화천월원평태차수감소,해마신경원조망솔、포장[Ca2+]i승고(P＜0.05);여O조비교,Sev조술후도피잠복기축단、평태상한정류시간연장화천월원평태차수증다,해마신경원조망솔、포장[Ca2+]i강저(P＜0.05).C조해마신경원포장초미결구미견이상,O조해마신경원포장초미결구병이학손상명현,Sev조해마신경원포장초미결구병이학손상감경.결론 2.4％칠불미예처리가감경노령대서술후인지공능장애,기궤제여조절개은태실형,강저해마신경원조망유관.
Objective To evaluate the effects of sevoflurane preconditioning on postoperative cognitive dysfunction in aged rats.Methods One hundred and twenty healthy male Sprague-Dawley rats,aged 18 months,weighing 400-450 g,were randomly divided into 3 groups (n =40 each) using a random number table:control group (group C),operation group (group O),and sevoflurane preconditioning group (group Sev).In group C,the rats were only anesthetized with pentobarbital sodium and did not undergo operation.In group O,the rats were anesthetized with pentobarbital sodium and underwent 30 min of exploratory laparotomy.In group Sev,the rats inhaled 2.4％ sevoflurane for 30 min and then inhaled air for 30 min,and the other procedures were similar to those previously described in group O.At 30 min before operation and on 1st,3rd,5th and 7th days after operation,Morris water maze test was performed to record the escape latency,time of staying at the original platform quadrant and frequency of crossing the original platform.At 30 min before operation and on 1st and 7th days after operation,10 rats in each group were sacrificed and hippocampi were isolated to detect the apoptotic rate and intracellular [Ca2 +] i (using flow cytometry) and the ultrastructure of hippocampal neurons was observed with transmission electron microscope.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant was shortened,the frequency of crossing the original platform was decreased,and the apoptotic rate and intracellular [Ca2 +] i were increased after operation in O and Sev groups (P ＜0.05).Compared with group O,the escape latency was significantly shorten,the time of staying at the original platform quadrant was prolonged,the frequency of crossing the original platform was increased,and the apoptotic rate and intracellular [Ca2+]i were decreased after operation in group Sev (P ＜ 0.05).Microscopic examination showed no abnormality in the ultrastructure of hippocampal neurons in group C,and the pathological changes of the ultrastructure of hippocampal neurons were obvious in group O,and were significantly attenuated in group Sev.Conclusion 2.4％ sevoflurane preconditioning can reduce postoperative cognitive dysfunction in aged rats,and regulation of imbalance of calcium homeostasis and reduction of cell apoptosis are involved in the mechanism.