CAJ | 학술논문

目的评价海马组蛋白乙酰化修饰在异氟醚致小鼠遗忘效应中的作用.方法 SPF级雄性C57BL/6J小鼠54只,8周龄,体重18 ～ 22 g,采用随机数字表法,将其分为3组(n=18):对照组(C组)、异氟醚组(ISO组)和组蛋白去乙酰化酶抑制剂丁酸钠组(SB组).C组吸人35％氧气30 min,ISO组和SB组吸入35％氧气和0.4％异氟醚的混合气体30 min,然后进行条件恐惧训练.训练结束后,C组和ISO组腹腔注射生理盐水6ml/kg,SB组腹腔注射丁酸钠1.2 g/kg.于条件恐惧训练结束后1h时,各组随机处死6只小鼠,取海马组织,采用Western blot法测定乙酰化组蛋白H3(Ac-H3) Ac-H4的表达.余小鼠于条件恐惧训练后24 h时,进行条件恐惧测试和旷场实验,记录凝滞时间、总路程和中央区停留时间.结果与C组比较,ISO组海马Ac-H3和Ac-H4的表达下调,测试阶段凝滞时间百分比降低(P＜0.05);与ISO组比较,SB组海马Ac-H3和Ac-H4的表达上调,测试阶段凝滞时间百分比升高(P＜0.05);3组间训练阶段凝滞时间百分比、总路程和中央区停留时间比较差异无统计学意义(P＞0.05).结论海马组蛋白乙酰化修饰参与调控异氟醚致小鼠的遗忘效应.
목적 평개해마조단백을선화수식재이불미치소서유망효응중적작용.방법 SPF급웅성C57BL/6J소서54지,8주령,체중18 ～ 22 g,채용수궤수자표법,장기분위3조(n=18):대조조(C조)、이불미조(ISO조)화조단백거을선화매억제제정산납조(SB조).C조흡인35％양기30 min,ISO조화SB조흡입35％양기화0.4％이불미적혼합기체30 min,연후진행조건공구훈련.훈련결속후,C조화ISO조복강주사생리염수6ml/kg,SB조복강주사정산납1.2 g/kg.우조건공구훈련결속후1h시,각조수궤처사6지소서,취해마조직,채용Western blot법측정을선화조단백H3(Ac-H3) Ac-H4적표체.여소서우조건공구훈련후24 h시,진행조건공구측시화광장실험,기록응체시간、총로정화중앙구정류시간.결과 여C조비교,ISO조해마Ac-H3화Ac-H4적표체하조,측시계단응체시간백분비강저(P＜0.05);여ISO조비교,SB조해마Ac-H3화Ac-H4적표체상조,측시계단응체시간백분비승고(P＜0.05);3조간훈련계단응체시간백분비、총로정화중앙구정류시간비교차이무통계학의의(P＞0.05).결론 해마조단백을선화수식삼여조공이불미치소서적유망효응.
Objective To evaluate the role of hippocampal histone acetylation in isoflurane-induced amnestic effect in mice.Methods Fifty-four male C57BL/6J mice,aged 8 weeks,weighing 18-22 g,were randomly divided into 3 groups (n =18 each) using a random number table:control group (group C),isoflurane group (group ISO) and histone deacetylase inhibitor sodium butyrate group (group SB).Group C inhaled 35％ oxygen for 30 ain,and ISO and SB groups inhaled the mixture of 35 ％ oxygen and 0.4％ isoflurane for 30 min,and then the animals underwent contextual fear conditioning training.After the end of training,normal saline 6 ml/kg was intraperitoneally injected in C and ISO groups,while in group SB,sodium butyrate 1.2 g/kg was intraperitoneally injected.One hour after the end of training,3 mice were sacrificed randomly in each group and their hippocampi were immediately removed for determination of the expression of acetylated histone-H3 (Ac-H3) and Ac-H4 by Western blot.Twenty-four hours after the end of training,contextual fear conditioning test and open field test were conducted.The freezing time,total distance and time of staying at the central zone were recorded.Results Compared with group C,Ac-H3 and Ac-H4 expression was significantly down-regulated,and the percentage of freezing time during testing was decreased in group ISO (P ＜ 0.05).Compared with group ISO,Ac-H3 and Ac-H4 expression was significantly up-regulated,and the percentage of freezing time during testing was increased in group SB (P ＜ 0.05).There was no significant difference in the percentage of freezing time during training,total distance and time of staying in the central zone among the 3 groups (P ＞ 0.05).Conclusion Hippocampal histone acetylation is involved in the regulation of isoflurane-induced amnestic effect in mice.