CAJ | 학술논문

目的探讨呼吸机相关性肺损伤大鼠肺组织巨噬细胞移动抑制因子(MIF) mRNA表达的变化.方法成年雄性SD大鼠30只,体重235 ～ 260 g,采用随机数字表法,将其分为3组(n=10):对照组(C组)、小潮气量机械通气组(S组)和大潮气量机械通气组(L组).C组气管插管后保持自主呼吸;S组和L组气管插管后行机械通气,S组潮气量7 ml/kg,L组潮气量40 ml/kg,吸呼比1:1,通气频率80次/min,FiO2 100％.自主呼吸或机械通气4h收集支气管肺泡灌洗液,测定总蛋白浓度和WBC计数,采用ELISA法测定MIF、IL-6、IL-1β的浓度;然后处死大鼠,取肺组织,光镜下观察病理学改变,测定湿重/干重比(W/D比),采用RT-PCR测定MIF mRNA表达.结果与C组和S组比较,L组BALF中WBC计数、总蛋白、MIF、IL-6、IL-1β的浓度和肺组织W/D比、MIF mRNA表达升高(P＜0.05),发生病理学损伤;C组和S组间上述各指标比较差异无统计学意义(P＞0.05).结论 MIF inRNA表达上调可能参与了大鼠呼吸机相关性肺损伤的发生.
목적 탐토호흡궤상관성폐손상대서폐조직거서세포이동억제인자(MIF) mRNA표체적변화.방법 성년웅성SD대서30지,체중235 ～ 260 g,채용수궤수자표법,장기분위3조(n=10):대조조(C조)、소조기량궤계통기조(S조)화대조기량궤계통기조(L조).C조기관삽관후보지자주호흡;S조화L조기관삽관후행궤계통기,S조조기량7 ml/kg,L조조기량40 ml/kg,흡호비1:1,통기빈솔80차/min,FiO2 100％.자주호흡혹궤계통기4h수집지기관폐포관세액,측정총단백농도화WBC계수,채용ELISA법측정MIF、IL-6、IL-1β적농도;연후처사대서,취폐조직,광경하관찰병이학개변,측정습중/간중비(W/D비),채용RT-PCR측정MIF mRNA표체.결과 여C조화S조비교,L조BALF중WBC계수、총단백、MIF、IL-6、IL-1β적농도화폐조직W/D비、MIF mRNA표체승고(P＜0.05),발생병이학손상;C조화S조간상술각지표비교차이무통계학의의(P＞0.05).결론 MIF inRNA표체상조가능삼여료대서호흡궤상관성폐손상적발생.
Objective To investigate the changes in the expression of macrophage migration inhibitory factor (MIF) mRNA in a rat model of ventilator-induced lung injury.Methods Thirty adult male Sprague-Dawley rats,weighing 2β5-260 g,were randomly divided into 3 groups (n =10 each) using a random number table:control group (group C),small tidal volume (VT) mechanical ventilation group (group S) and large tidal volume mechanical ventilation group (group L).The animals were anesthetized with intraperitoneal ketamine 100 mg/kg,midazolam 0.2 mg/kg and atropine 1.0 mg/kg.The rats were tracheostomized and spontaneous breathing was maintained in group C,while the rats were tracheostomized and mechanically ventilated for 4 h in groups S and L.The tidal volume was 7 ml/kg (group S) or 40 ml/kg (group L),I ∶ E was 1 ∶ 1,RR was 80 bpm and FiO2 was 100％.At 4 h of spontaneous breathing or mechanical ventilation,broncho-alveolar lung lavage fluid (BALF) was collected for determination of the total protein concentration,white blood cell (WBC) counts and concentrations of MIF,IL-6 and IL-1β (by ELISA).Then the rats were sacrificed and the lungs removed for microscopic examination and for determination of wet to dry lung weight ratio (W/D ratio) and expression of MIF mRNA (by RT-PCR).Results Compared with C and S groups,WBC counts,concentrations of total protein,MIF,IL-6 and IL-1β in BALF,and W/D ratio and expression of MIF mRNA in lung tissues were significantly increased in group L (P ＜ 0.05).There was no significant difference in the indexes mentioned above between group C and group S (P ＞ 0.05).The pathological changes occurred in group L.Conclusion The up-regulation of MIF mRNA expression in lung tissues may be involved in the development of ventilator-induced lung injury in rats.