中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
1期
43-46
,共4页
钱黎%邓云坤%曾祥刚%丁济飞
錢黎%鄧雲坤%曾祥剛%丁濟飛
전려%산운곤%증상강%정제비
褪黑激素%神经痛,带状疱疹后
褪黑激素%神經痛,帶狀皰疹後
퇴흑격소%신경통,대상포진후
Melatonin%Neuralgia,postherpetic
目的 评价褪黑素与大鼠带状疱疹后神经痛(PHN)形成的关系.方法 成年Wistar大鼠60只,雌雄不拘,3月龄,体重180 ～ 230 g,采用随机数定表法,将其分为6组(n=10).A组:假手术并接种灭活Ⅰ型单纯疱疹病毒(HSV-1)；B组:假手术并接种HSV-1；C组:松果体摘除并接种灭活HSV-1;D组:松果体摘除并接种HSV-1；E组:松果体摘除并接种灭活HSV-1,每天腹腔注射褪黑素；F组:松果体摘除并接种HSV-1,每天给予褪黑素.松果体摘除术后1h于左后肢胫部皮下接种HSV-1或灭活HSV-1,褪黑素剂量120 mg/kg,1次/d.分别于接种HSV-1前、接种后1、5、10、20、30 d时测定机械痛觉反应评分(MPRS).接种HSV-1后30 d时记录PHN发生情况.结果 与A组比较,B组MPRS评分升高(P＜0.05)；与B组比较,D组MPRS评分升高(P＜0.05)；与D组比较,F组MPRS评分降低(P＜0.05).A组、C组和E组均未发生PHN,B组、D组和F组PHN的发生率分别为40％、90％和40％；与B组比较,D组PHN发生率升高(P＜0.05),F组差异无统计学意义(P＞0.05).结论 褪黑素分泌减少与大鼠PHN形成有关.
目的 評價褪黑素與大鼠帶狀皰疹後神經痛(PHN)形成的關繫.方法 成年Wistar大鼠60隻,雌雄不拘,3月齡,體重180 ～ 230 g,採用隨機數定錶法,將其分為6組(n=10).A組:假手術併接種滅活Ⅰ型單純皰疹病毒(HSV-1)；B組:假手術併接種HSV-1；C組:鬆果體摘除併接種滅活HSV-1;D組:鬆果體摘除併接種HSV-1；E組:鬆果體摘除併接種滅活HSV-1,每天腹腔註射褪黑素；F組:鬆果體摘除併接種HSV-1,每天給予褪黑素.鬆果體摘除術後1h于左後肢脛部皮下接種HSV-1或滅活HSV-1,褪黑素劑量120 mg/kg,1次/d.分彆于接種HSV-1前、接種後1、5、10、20、30 d時測定機械痛覺反應評分(MPRS).接種HSV-1後30 d時記錄PHN髮生情況.結果 與A組比較,B組MPRS評分升高(P＜0.05)；與B組比較,D組MPRS評分升高(P＜0.05)；與D組比較,F組MPRS評分降低(P＜0.05).A組、C組和E組均未髮生PHN,B組、D組和F組PHN的髮生率分彆為40％、90％和40％；與B組比較,D組PHN髮生率升高(P＜0.05),F組差異無統計學意義(P＞0.05).結論 褪黑素分泌減少與大鼠PHN形成有關.
목적 평개퇴흑소여대서대상포진후신경통(PHN)형성적관계.방법 성년Wistar대서60지,자웅불구,3월령,체중180 ～ 230 g,채용수궤수정표법,장기분위6조(n=10).A조:가수술병접충멸활Ⅰ형단순포진병독(HSV-1)；B조:가수술병접충HSV-1；C조:송과체적제병접충멸활HSV-1;D조:송과체적제병접충HSV-1；E조:송과체적제병접충멸활HSV-1,매천복강주사퇴흑소；F조:송과체적제병접충HSV-1,매천급여퇴흑소.송과체적제술후1h우좌후지경부피하접충HSV-1혹멸활HSV-1,퇴흑소제량120 mg/kg,1차/d.분별우접충HSV-1전、접충후1、5、10、20、30 d시측정궤계통각반응평분(MPRS).접충HSV-1후30 d시기록PHN발생정황.결과 여A조비교,B조MPRS평분승고(P＜0.05)；여B조비교,D조MPRS평분승고(P＜0.05)；여D조비교,F조MPRS평분강저(P＜0.05).A조、C조화E조균미발생PHN,B조、D조화F조PHN적발생솔분별위40％、90％화40％；여B조비교,D조PHN발생솔승고(P＜0.05),F조차이무통계학의의(P＞0.05).결론 퇴흑소분비감소여대서PHN형성유관.
Objective To evaluate the relationship between melatonin and development of post-herpetic neuralgia (PHN) in rats.Methods Sixty adult Wistar rats of both sexes,aged 3 months,weighing 180-230 g,were randomly divided into A-F 6 groups (n =10 each) using a random number table.Group A served as sham operation group,and inactivated herpes simplex virus type-1 (HSV-1) was inoculated.Group B served as sham operation group,and HSV-1 was inoculated.In group C,pinealectomy was performed and inactivated HSV-1 was inoculated.In group D,pinealectomy was performed,and HSV-1 was inoculated.In group E,pinealectomy was performed,inactivated HSV-1 was inoculated,and melatonin was injected intraperitoneally everyday.In group F,pinealectomy was performed,HSV-1 was inoculated,and melatonin was injected intraperitoneally everyday.HSV-1 or inactivated HSV-1 was inoculated on the left hind paw of the rats at 1 h after pinealectomy,and the dosage of melatonin was 120 mg/kg once a day.The mechanical pain response score (MPRS) was determined before inoculation and on days 1,5,10,20 and 30 post-inoculation.The development of PHN was recorded on day 30 post-inoculation.Results MPRS was significantly higher in group B than in group A,and in group D than in group B (P ＜ 0.05).MPRS was significantly lower in group F than in group D (P ＜ 0.05).PHN was not found in A,C and E groups,and the incidence of PHN was 40％,90％ and 40％,respectively.Compared with group B,the incidence of PHN was significantly increased in group D (P ＜ 0.05),and no significant change was found in group F (P ＞ 0.05).Conclusion Reduction of melatonin secretion is related to the development of PHN in rats.
