中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
1期
98-101
,共4页
张静%王琛%胡素梅%曹建方%谢红%朱江%孙亦晖
張靜%王琛%鬍素梅%曹建方%謝紅%硃江%孫亦暉
장정%왕침%호소매%조건방%사홍%주강%손역휘
麻醉药,吸入%心肌再灌注损伤%细胞死亡%细胞外信号调节MAP激酶类
痳醉藥,吸入%心肌再灌註損傷%細胞死亡%細胞外信號調節MAP激酶類
마취약,흡입%심기재관주손상%세포사망%세포외신호조절MAP격매류
Anesthetics,inhalation%Myocardial reperfusion injury%Cell death%Extracellular signal-regulated MAP kinases
目的 评价七氟醚后处理对大鼠缺血再灌注心肌细胞胀亡和凋亡的影响及细胞外信号调节激酶(ERK) 1/2信号转导通路在其中的作用.方法 取Langendorff离体灌注模型成功的大鼠心脏72个,采用随机数字表法分为6组(n=12):假手术组(S组)、缺血再灌注组(I/R组)、七氟醚后处理组(SP组)、ERK1/2抑制剂PD98059组(PD组)、PD98059溶剂二甲基亚砜组(DMSO组)和七氟醚后处理+ PD98059组(SP+ PD组).除S组,其余各组心脏缺血30 min,再灌注2h.SP组、DMSO组和PD组分别于缺血末至再灌注15 min灌注经3.0%七氟醚、DMSO(< 0.2%)和PD98059(20 μmol/L)饱和的K-H液,随后更换为正常K-H液再灌注105 min.SP+ PD组于缺血末至再灌注15 min灌注经3.0%七氟醚和PD98059(20 μmol/L)饱和的K-H液.再灌注末测定心肌梗死体积,采用Western blot法检测porimin和caspase-8蛋白表达水平.结果 与S组比较,其余各组心肌梗死体积增加,porimin和caspase-8表达上调(P<0.05);与I/R组比较,SP组梗死体积减少,porimin和caspase-8表达下调(P<0.05),其余各组各指标差异无统计学意义(P>0.05).结论 七氟醚后处理可激活ERK1/2信号转导通路,抑制心肌细胞胀亡和凋亡,从而减轻大鼠离体心脏缺血再灌注损伤.
目的 評價七氟醚後處理對大鼠缺血再灌註心肌細胞脹亡和凋亡的影響及細胞外信號調節激酶(ERK) 1/2信號轉導通路在其中的作用.方法 取Langendorff離體灌註模型成功的大鼠心髒72箇,採用隨機數字錶法分為6組(n=12):假手術組(S組)、缺血再灌註組(I/R組)、七氟醚後處理組(SP組)、ERK1/2抑製劑PD98059組(PD組)、PD98059溶劑二甲基亞砜組(DMSO組)和七氟醚後處理+ PD98059組(SP+ PD組).除S組,其餘各組心髒缺血30 min,再灌註2h.SP組、DMSO組和PD組分彆于缺血末至再灌註15 min灌註經3.0%七氟醚、DMSO(< 0.2%)和PD98059(20 μmol/L)飽和的K-H液,隨後更換為正常K-H液再灌註105 min.SP+ PD組于缺血末至再灌註15 min灌註經3.0%七氟醚和PD98059(20 μmol/L)飽和的K-H液.再灌註末測定心肌梗死體積,採用Western blot法檢測porimin和caspase-8蛋白錶達水平.結果 與S組比較,其餘各組心肌梗死體積增加,porimin和caspase-8錶達上調(P<0.05);與I/R組比較,SP組梗死體積減少,porimin和caspase-8錶達下調(P<0.05),其餘各組各指標差異無統計學意義(P>0.05).結論 七氟醚後處理可激活ERK1/2信號轉導通路,抑製心肌細胞脹亡和凋亡,從而減輕大鼠離體心髒缺血再灌註損傷.
목적 평개칠불미후처리대대서결혈재관주심기세포창망화조망적영향급세포외신호조절격매(ERK) 1/2신호전도통로재기중적작용.방법 취Langendorff리체관주모형성공적대서심장72개,채용수궤수자표법분위6조(n=12):가수술조(S조)、결혈재관주조(I/R조)、칠불미후처리조(SP조)、ERK1/2억제제PD98059조(PD조)、PD98059용제이갑기아풍조(DMSO조)화칠불미후처리+ PD98059조(SP+ PD조).제S조,기여각조심장결혈30 min,재관주2h.SP조、DMSO조화PD조분별우결혈말지재관주15 min관주경3.0%칠불미、DMSO(< 0.2%)화PD98059(20 μmol/L)포화적K-H액,수후경환위정상K-H액재관주105 min.SP+ PD조우결혈말지재관주15 min관주경3.0%칠불미화PD98059(20 μmol/L)포화적K-H액.재관주말측정심기경사체적,채용Western blot법검측porimin화caspase-8단백표체수평.결과 여S조비교,기여각조심기경사체적증가,porimin화caspase-8표체상조(P<0.05);여I/R조비교,SP조경사체적감소,porimin화caspase-8표체하조(P<0.05),기여각조각지표차이무통계학의의(P>0.05).결론 칠불미후처리가격활ERK1/2신호전도통로,억제심기세포창망화조망,종이감경대서리체심장결혈재관주손상.
Objective To evaluate the effects of sevoflurane postconditioning on the oncosis and apoptosis in cardiomyocytes during ischemia-reperfusion (I/R) in isolated rat hearts and the role of extracellular signalregulated protein kinase 1/2 (ERK1/2) signal transduction pathway in it.Methods Seventy-two isolated rat hearts perfused in a Langendorff apparatus were randomly divided into 6 groups (n =12 each) using a random number table:sham operation group (group S),myocardial I/R group (group I/R),sevoflurane postconditioning group (group SP),PD98059 vehicle dimethyl sulfoxide (DMSO) group (group DMSO),selective ERK1/2 inhibitor PD98059 group (group PD),and sevoflurane postconditioning + PD98059 group (group SP + PD).The hearts were subjected to ischemia for 30 min followed by 2 h reperfusion in the other groups except group S.In SP,DMSO and PD groups,the hearts were perfused with K-H solution saturated with 3.0% sevoflurane,DMSO (<0.2%) and PD98059 (20 μmol/L),respectively,for 15 min starting from the end of ischemia until 15 min of reperfusion,and then with plain K-H solution for 105 min.In group SP+ PD,the hearts were perfused with K-H solution saturated with 3.0% sevoflurane and PD98059 for 15 min starting from the end of ischemia until 15 min of reperfusion.Myocardial infarct size and expression of porimin and caspase-8 proteins (by Western blot) were measured at the end of reperfusion.Results Compared with S group,the myocardial infarct size was significantly increased,and the expression of porimin and caspase-8 proteins was up-regulated in the other groups (P < 0.05).Compared with I/R group,the myocardial infarct size was significantly decreased,and the expression of porimin and caspase-8 proteins was down-regulated in group SP (P < 0.05),and no significant changes were found in the other groups (P > 0.05).Conclusion Sevoflurane postconditioning can activate ERK1/2 signal transduction pathway and inhibit the oncosis and apoptosis in cardiomyocytes,thus attenuating I/R injury in isolated rat hearts.