CAJ | 학술논문

目的评价姜黄素预处理对大鼠肠缺血再灌注时黄嘌呤氧化酶活性的影响.方法清洁级雌性SD大鼠30只,体重180 ～ 220 g,采用随机数字表法,将其分为3组(n=10):假手术组(S组)、肠缺血再灌注组(I/R组)和姜黄素预处理组(Cur组).采用夹闭肠系膜上动脉75 min后恢复灌注的方法制备大鼠肠缺血再灌注损伤模型.夹闭肠系膜上动脉前5d,Cur组每天胃饲姜黄素200mg/kg(采用生理盐水稀释),S组和I/R组胃饲等容量生理盐水.于再灌注4h时放血处死大鼠,取肠组织标本,光镜下观察病理学结果并行Chiu评分,采用分光光度法检测黄嘌呤氧化酶活性,硫酸代巴比妥酸法测定丙二醛含量,黄嘌呤氧化酶法检测超氧化物歧化酶活性.结果与S组比较,I/R组和Cur组大鼠肠组织Chiu评分、黄嘌呤氧化酶活性、丙二醛含量升高,超氧化物歧化酶活性降低(P＜0.05)；与I/R组比较,Cur组大鼠肠组织Chiu评分、黄嘌呤氧化酶活性、丙二醛含量降低,超氧化物歧化酶活性升高(P＜0.05).结论姜黄素预处理可减轻大鼠肠缺血再灌注损伤,可能与其抑制肠组织黄嘌呤氧化酶活性,从而降低氧化应激反应有关.
목적 평개강황소예처리대대서장결혈재관주시황표령양화매활성적영향.방법 청길급자성SD대서30지,체중180 ～ 220 g,채용수궤수자표법,장기분위3조(n=10):가수술조(S조)、장결혈재관주조(I/R조)화강황소예처리조(Cur조).채용협폐장계막상동맥75 min후회복관주적방법제비대서장결혈재관주손상모형.협폐장계막상동맥전5d,Cur조매천위사강황소200mg/kg(채용생리염수희석),S조화I/R조위사등용량생리염수.우재관주4h시방혈처사대서,취장조직표본,광경하관찰병이학결과병행Chiu평분,채용분광광도법검측황표령양화매활성,류산대파비타산법측정병이철함량,황표령양화매법검측초양화물기화매활성.결과 여S조비교,I/R조화Cur조대서장조직Chiu평분、황표령양화매활성、병이철함량승고,초양화물기화매활성강저(P＜0.05)；여I/R조비교,Cur조대서장조직Chiu평분、황표령양화매활성、병이철함량강저,초양화물기화매활성승고(P＜0.05).결론 강황소예처리가감경대서장결혈재관주손상,가능여기억제장조직황표령양화매활성,종이강저양화응격반응유관.
Objective To evaluate the effects of curcumin preconditioning on the activity of xanthine oxidase (XOD) during intestinal ischemia-reperfusion (I/R) in rats.Methods Thirty female Sprague-Dawley rats,aged 180-220 g,were randomly divided into 3 groups (n =10 each) using a random number table:sham operation group (S group),intestinal I/R group (I/R group),and curcumin preconditioning group (Cur group).Intestinal I/R was induced by clamping the superior mesenteric artery for 75 min followed by reperfusion.Curcumin 200 mg/kg was given everyday for 5 days before intestinal I/R in Cur group and the equal volume of normal saline was given instead of curcumin in S and I/R groups.The rats were sacrificed at 4 h of reperfusion and the intestinal specimens were obtained for microscopic examination of pathologic changes which were graded using Chiu scoring system and for determination of XOD activity,content of malondialdehyde (MDA),and superoxide dismutase (SOD) activity.Results Compared with S group,the Chiu score,activity of XOD and content of MDA were significantly increased,while the activity of SOD was decreased in I/R and Cur groups (P ＜ 0.05).Compared with I/R group,the Chiu score,activity of XOD and content of MDA were significantly decreased,and the activity of SOD was increased in Cur group (P ＜ 0.05).Conclusion Curcumin preconditioning can attenuate intestinal I/R injury in rats,which may be due to inhibition of XOD activity and decreased oxidative stress in intestinal tissues.