CAJ | 학술논문

目的探讨七氟醚麻醉对老龄大鼠海马胶质细胞活化的影响.方法健康雄性Wistar大鼠104只,体重600 ～ 650 g,19 ～ 20月龄,采用随机数字表法分为2组(n=52):对照组(C组)和七氟醚组(S组).S组行胫骨骨折切开复位内固定手术,吸入3.6％七氟醚2h进行麻醉.于术后1、3和7d进行Y迷宫实验和恐惧条件化训练实验,并取海马组织,采用免疫荧光法检测小胶质细胞和星形胶质细胞的活化程度,采用Western blot法检测IL-6和TNF-α的表达水平.结果与C组比较,S组各个臂内穿梭次数总和减少,N臂停留时间缩短,条件诱导僵直时间百分比和场景相关僵直时间百分比降低,海马小胶质细胞和星形胶质细胞活化水平升高,海马IL-6和TNF-α表达上调(P＜0.05).结论七氟醚麻醉诱发的老龄大鼠术后认知功能障碍可能与增强海马胶质细胞活化有关.
목적 탐토칠불미마취대노령대서해마효질세포활화적영향.방법 건강웅성Wistar대서104지,체중600 ～ 650 g,19 ～ 20월령,채용수궤수자표법분위2조(n=52):대조조(C조)화칠불미조(S조).S조행경골골절절개복위내고정수술,흡입3.6％칠불미2h진행마취.우술후1、3화7d진행Y미궁실험화공구조건화훈련실험,병취해마조직,채용면역형광법검측소효질세포화성형효질세포적활화정도,채용Western blot법검측IL-6화TNF-α적표체수평.결과 여C조비교,S조각개비내천사차수총화감소,N비정류시간축단,조건유도강직시간백분비화장경상관강직시간백분비강저,해마소효질세포화성형효질세포활화수평승고,해마IL-6화TNF-α표체상조(P＜0.05).결론 칠불미마취유발적노령대서술후인지공능장애가능여증강해마효질세포활화유관.
Objective To evaluate the effect of sevoflurane anesthesia on activation of gliocytes in aged rat hippocampi.Methods One hundred and four male Wistar rats,weighing 600-650 g,were randomly divided into 2 groups (n =52 each) using a random number table:control group (group C) and sevoflurane group (group S).Group S inhaled 3.6％ sevoflurane for 2 h,and open reduction and internal fixation was performed after tibial fracture was induced.Y-maze test and fear conditioning test were carried out on days 1,3 and 7 after surgery.Six rats of each group were chosen on days 1,3 and 7 after surgery and hippocampi were removed to detect the activation of microglias and astrocytes (by immunofluorescence) and expression of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) (by Western blot).Results Compared with group C,the number of entries into each arm was significantly reduced,the duration of N arm stay was shortened,the percentage of time spent freezing induced by condition and the percentage of time spent freezing induced by context were decreased,and the activation of microglias and astrocytes was increased,and the expression of IL-6 and TNF-α was up-regulated in S groups (P ＜ 0.05).Conclusion Sevoflurane-induced postoperative cognitive dysfunction may be related to enhanced activation of gliocytes in aged rat hippocampi.