CAJ | 학술논문

目的评价不同浓度帕瑞昔布钠对大鼠离体精子运动功能、体外获能和顶体反应的影响.方法通过扩散法收集SD大鼠附睾精子,采用随机数字表法分为4组(n=18):对照组(C组)、100、500和1 000μmol/L帕瑞昔布钠组(P1-3组).在37℃孵箱中培养5h后,采用计算机辅助精子分析系统评价精子运动功能,记录精子运动活力[(a+b)％]、平均速度(VAP)、直线速度(VSL)、曲线速度(VCL)和侧摆幅度(ALH),采用金霉素荧光染色技术评价精子体外获能情况,采用考马斯亮蓝染色法评价精子顶体反应情况.结果 C组、P1-3组精子VAP、VSL、VCL和精子获能率依次降低(P＜0.05).与C组比较,P2.3组(a+b)％和P2组ALH降低(P＜0.05).各组精子顶体反应率比较差异无统计学意义(P＞0.05).结论帕瑞昔布钠呈浓度依赖性抑制大鼠离体精子运动功能和体外获能,对顶体反应无明显影响.
목적 평개불동농도파서석포납대대서리체정자운동공능、체외획능화정체반응적영향.방법 통과확산법수집SD대서부고정자,채용수궤수자표법분위4조(n=18):대조조(C조)、100、500화1 000μmol/L파서석포납조(P1-3조).재37℃부상중배양5h후,채용계산궤보조정자분석계통평개정자운동공능,기록정자운동활력[(a+b)％]、평균속도(VAP)、직선속도(VSL)、곡선속도(VCL)화측파폭도(ALH),채용금매소형광염색기술평개정자체외획능정황,채용고마사량람염색법평개정자정체반응정황.결과 C조、P1-3조정자VAP、VSL、VCL화정자획능솔의차강저(P＜0.05).여C조비교,P2.3조(a+b)％화P2조ALH강저(P＜0.05).각조정자정체반응솔비교차이무통계학의의(P＞0.05).결론 파서석포납정농도의뢰성억제대서리체정자운동공능화체외획능,대정체반응무명현영향.
Objective To evaluate the effects of different concentrations of parecoxib sodium on the rat sperm motility,capacitation and acrosome reaction in vitro.Methods The sperm samples from Sprague-Dawley rat epididymis were collected by Klinefelter diffusion method and randomly divided into 4 groups (n =18 each) using a random number table:control group (group C),and parecoxib sodium 100,500,1 000 μmol/L groups (P1-3 groups).Parecoxib sodium with the final concentrations of 100,500 and 1 000 μmol/L was added to the culture medium.The samples were then incubated for 5 h in an airtight container filled with 5 ％ CO2 at 37 ℃.Then sperm motility was examined in vitro at 37 ℃ and analyzed by the computer-assisted sperm analysis,including the sperm motility ((a + b)％),average path velocity (VAP),straight line velocity (VSL),curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH).The capacitation effect was assessed by using the chlortetracycline staining and phase-contract microscopy.The acrosome reaction was evaluated by coomassie brilliant blue staining.Results The VAP,VSL,VCL and capacitation ability of the sperm were gradually decreased in C and P1-3 groups (P ＜ 0.05).Compared with group C,(a + b)％ in P2,3 groups and ALH in P2 group were significantly decreased (P ＜ 0.05).There was no significant difference in the acrosome reaction between groups (P ＞ 0.05).Conclusion Parecoxib sodium has significant inhibitory effects on the rat sperm motility and capacitation in a dose-dependent manner,while has no effect on the acrosome reaction in vitro.