CAJ | 학술논문

目的评价吗啡依赖大鼠中脑远位触液神经元细胞外信号调节蛋白激酶5(ERK5)活性的变化.方法成年雄性SD大鼠48只,体重230 ～ 270 g,采用随机数字表法,将其分为2组(n=24):对照组(A组)和吗啡依赖组(B组).皮下注射吗啡,每天2次,连续5 d,建立大鼠吗啡依赖模型.每日剂量为:首日10 mg/kg,第2～5天分别为20、30、40和50 mg/kg.A组皮下注射等容量生理盐水.吗啡依赖模型制备的第3天上午,经侧脑室注射30％霍乱毒素亚单位B与辣根过氧化物酶复合物(CB-HRP)3 μl,用于标记远位触液神经元.于末次皮下注射吗啡后4h,取中脑远位触液神经元所在节段组织,采用免疫荧光法计数CB-HRP阳性神经元、磷酸化ERK5(p-ERK5)阳性神经元及CB-HRP/p-ERK5阳性神经元.结果与A组相比,B组中脑p-ERK5阳性神经元计数和CB-HRP/p-ERK5阳性神经元计数升高(P＜0.05),CB-HRP阳性神经元计数差异无统计学意义(P＞0.05).结论中脑远位触液神经元ERK5活性增强可能参与了大鼠吗啡依赖的形成.
목적 평개마배의뢰대서중뇌원위촉액신경원세포외신호조절단백격매5(ERK5)활성적변화.방법 성년웅성SD대서48지,체중230 ～ 270 g,채용수궤수자표법,장기분위2조(n=24):대조조(A조)화마배의뢰조(B조).피하주사마배,매천2차,련속5 d,건립대서마배의뢰모형.매일제량위:수일10 mg/kg,제2～5천분별위20、30、40화50 mg/kg.A조피하주사등용량생리염수.마배의뢰모형제비적제3천상오,경측뇌실주사30％곽란독소아단위B여랄근과양화물매복합물(CB-HRP)3 μl,용우표기원위촉액신경원.우말차피하주사마배후4h,취중뇌원위촉액신경원소재절단조직,채용면역형광법계수CB-HRP양성신경원、린산화ERK5(p-ERK5)양성신경원급CB-HRP/p-ERK5양성신경원.결과 여A조상비,B조중뇌p-ERK5양성신경원계수화CB-HRP/p-ERK5양성신경원계수승고(P＜0.05),CB-HRP양성신경원계수차이무통계학의의(P＞0.05).결론 중뇌원위촉액신경원ERK5활성증강가능삼여료대서마배의뢰적형성.
Objective To evaluate the changes in the activity of extracellular signal-regulated kinase 5 (ERK5) in the distal cerebrospinal fluid contacting neurons (CSF-CNs) in the mid-brain of morphine dependent rats.Methods Forty-eight male adult Sprague-Dawley rats weighing 230-270 g,were randomly divided into 2 groups (n =24 each) using a random number table:control group (group A) and morphine dependence group (group B).Morphine dependence was induced by increasing doses of subautaneous morphine for 5 consecutive days.The initial dose of morphine was 10 mg/kg twice a day and was increased by 10 mg/kg everyday until 50 mg/kg on 5th dav.The equal volume of normal saline was injected subcutaneously instead of morphine in group A.On 3rd day after morphine dependence was induced,the distal CSF-CNs in the mid-brain was labeled with 30％ cholera toxin subunit B and horseradish peroxidase compound (CB-HRP) 3 μl injected in the lateral cerebral ventricle in the morning.At 4 h after the last injection of morphine,the segments in which CSF-CNs were located were removed,and CB-HRP positive neurons,phosphor-ERK5 (p-ERK5) positive neurons and CB-HRP/p-ERK5 positive neurons were counted.Results Compared with group A,the number of p-ERK5 and CB-HRP/p-ERK5 positive neurons in the mid-brain was significantly increased (P ＜ 0.05),and no significant change was found in CB-HRP positive neurons in group B (P ＞ 0.05).Conclusion The enhanced activity of ERK5 in the distal CSFCNs in the mid-brain may contribute to the development of morphine dependence in rats.