中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
2期
216-218
,共3页
张冬梅%徐向钊%张冰%王建珍%王海滨
張鼕梅%徐嚮釗%張冰%王建珍%王海濱
장동매%서향쇠%장빙%왕건진%왕해빈
胺碘酮%心肌再灌注损伤%连接蛋白43
胺碘酮%心肌再灌註損傷%連接蛋白43
알전동%심기재관주손상%련접단백43
Amiodarone%Myocardial reperfusion injury%Connexin 43
目的 评价胺碘酮对大鼠心肌缺血再灌注时缝隙连接蛋白43(Cx43)表达的影响.方法 成年雄性SD大鼠36只,体重250～ 300 g,采用随机数字表法,将其分为3组(n=12):假手术组(S组)、缺血再灌注组(I/R组)和胺碘酮组(AM组).I/R组和AM组采用结扎左冠状动脉前降支30 min,再灌注120 min的方法制备大鼠心肌缺血再灌注损伤模型.AM组于缺血前10 min静脉注射胺碘酮5.0 mg/kg后以0.15 mg·kg-1·min-的速率输注至再灌注末.记录再灌注期间心律失常的发生情况,并进行心律失常评分.于再灌注120 min时,股动脉取血样,采用ELISA法测定血清cTnI和CK-MB的浓度;然后处死大鼠,取心肌组织,采用免疫组化法检测Cx43蛋白表达,采用RT-PCR法检测Cx43mRNA表达.结果 与S组比较,I/R组和AM组心律失常评分、血清CK-MB和cTnI的水平均升高,心肌组织Cx43蛋白及其mRNA表达下调(P＜0.05);与I/R组比较,AM组心律失常评分、血清CK-MB和cTnl的水平均降低,心肌组织Cx43蛋白及其mRNA表达上调(P＜0.05).S组和AM组Cx43呈条带状规律分布,多数位于闰盘处;I/R组Cx43呈弥散点或块状分布,闰盘处少见.结论 胺碘酮减轻大鼠心肌缺血再灌注性心律失常的机制与抑制心肌Cx43的再分布和上调Cx43的表达有关.
目的 評價胺碘酮對大鼠心肌缺血再灌註時縫隙連接蛋白43(Cx43)錶達的影響.方法 成年雄性SD大鼠36隻,體重250～ 300 g,採用隨機數字錶法,將其分為3組(n=12):假手術組(S組)、缺血再灌註組(I/R組)和胺碘酮組(AM組).I/R組和AM組採用結扎左冠狀動脈前降支30 min,再灌註120 min的方法製備大鼠心肌缺血再灌註損傷模型.AM組于缺血前10 min靜脈註射胺碘酮5.0 mg/kg後以0.15 mg·kg-1·min-的速率輸註至再灌註末.記錄再灌註期間心律失常的髮生情況,併進行心律失常評分.于再灌註120 min時,股動脈取血樣,採用ELISA法測定血清cTnI和CK-MB的濃度;然後處死大鼠,取心肌組織,採用免疫組化法檢測Cx43蛋白錶達,採用RT-PCR法檢測Cx43mRNA錶達.結果 與S組比較,I/R組和AM組心律失常評分、血清CK-MB和cTnI的水平均升高,心肌組織Cx43蛋白及其mRNA錶達下調(P＜0.05);與I/R組比較,AM組心律失常評分、血清CK-MB和cTnl的水平均降低,心肌組織Cx43蛋白及其mRNA錶達上調(P＜0.05).S組和AM組Cx43呈條帶狀規律分佈,多數位于閏盤處;I/R組Cx43呈瀰散點或塊狀分佈,閏盤處少見.結論 胺碘酮減輕大鼠心肌缺血再灌註性心律失常的機製與抑製心肌Cx43的再分佈和上調Cx43的錶達有關.
목적 평개알전동대대서심기결혈재관주시봉극련접단백43(Cx43)표체적영향.방법 성년웅성SD대서36지,체중250～ 300 g,채용수궤수자표법,장기분위3조(n=12):가수술조(S조)、결혈재관주조(I/R조)화알전동조(AM조).I/R조화AM조채용결찰좌관상동맥전강지30 min,재관주120 min적방법제비대서심기결혈재관주손상모형.AM조우결혈전10 min정맥주사알전동5.0 mg/kg후이0.15 mg·kg-1·min-적속솔수주지재관주말.기록재관주기간심률실상적발생정황,병진행심률실상평분.우재관주120 min시,고동맥취혈양,채용ELISA법측정혈청cTnI화CK-MB적농도;연후처사대서,취심기조직,채용면역조화법검측Cx43단백표체,채용RT-PCR법검측Cx43mRNA표체.결과 여S조비교,I/R조화AM조심률실상평분、혈청CK-MB화cTnI적수평균승고,심기조직Cx43단백급기mRNA표체하조(P＜0.05);여I/R조비교,AM조심률실상평분、혈청CK-MB화cTnl적수평균강저,심기조직Cx43단백급기mRNA표체상조(P＜0.05).S조화AM조Cx43정조대상규률분포,다수위우윤반처;I/R조Cx43정미산점혹괴상분포,윤반처소견.결론 알전동감경대서심기결혈재관주성심률실상적궤제여억제심기Cx43적재분포화상조Cx43적표체유관.
Objective To evaluate the effects of amiodarone on connexin43 (Cx43) expression during myocardial ischemia-reperfusion (I/R) in rats.Methods Adult male Sprague-Dawley rats,weighing 250-300 g,were randomly divided into 3 groups (n =12 each) using a random number table:shame operation group (group S),I/R group and amiodarone group (group AM).Myocardial ischemia was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 120 min of reperfusion in I/R and AM groups.Amiodarone 5.0 mg/kg was injected intravenously at 10 min before ischemia followed by infusion at a rate of 0.15 mg· kg-1 · min 1 until the end of reperfusion.Arrhythmia was recorded during reperfusion and scored.At the end of reperfusion,blood samples were taken from the femoral artery for determination of the serum levels of cardiac troponin Ⅰ (cTnI) and creatine kinase isoenzyme-MB (CK-MB).Then the animals were sacrificed and myocardial specimens were removed for determination of the expression of Cx43 protein (by immuno-histochemistry) and mRNA (by RT-PCR) in myocardial tissues.Results Compared with group S,the arrhythmia score and serum levels of CK-MB and cTnl were significantly increased,and the expression of Cx43 protein and mRNA was downregulated in I/R and AM groups (P ＜ 0.05).Compared with group I/R,the arrhythmia score and serum levels of CK-MB and cTnI were significantly decreased,and the expression of Cx43 protein and mRNA was up-regulated in group AM (P ＜ 0.05).Myocardial Cx43 was unevenly distributed in group I/R,while evenly distributed in S and AM groups.Conclusion The mechanism by which amiodarone protects myocardium against I/R-induced arrhythmia is related to inhibition of redistribution of Cx43 and up-regulation of Cx43 expression in rats.
