中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
2期
241-244
,共4页
亚甲蓝%脓毒症%肾
亞甲藍%膿毒癥%腎
아갑람%농독증%신
Methylene blue%Sepsis%Kindey
目的 评价亚甲蓝预先给药对脓毒症诱发大鼠急性肾损伤的影响.方法 雄性SD大鼠90只,1.5 ～ 2.5月龄,体重200～ 250 g,采用随机数字表法,将其分为3组(n=30):假手术组(S组)、盲肠结扎穿孔组(CLP组)和亚甲蓝组(MB组).S组和CLP组尾静脉注射生理盐水0.8 ml;MB组尾静脉注射10％亚甲蓝15 mg/kg(用生理盐水稀释至0.8 ml).给药结束后CLP组和MB组采用盲肠结扎穿孔法制备大鼠脓毒症模型.每组取20只大鼠,观察术后72 h内生存情况.术后18 h时每组处死10只大鼠,取肾组织,光镜下观察病理学结果,分别采用免疫组化法和Western blot法测定多聚(ADP-核糖)聚合酶(PARP-1)的表达水平;经心脏取血样,测定血清尿素氮、肌酐、半胱氨酸蛋白酶抑制剂C(cystatin C)和中性粒细胞明胶酶相关载脂蛋白(NGAL)的浓度.结果 与S组比较,CLP组和MB组术后24、48和72 h时的生存率降低,血清尿素氮、肌酐、NGAL和cystatin C的浓度升高,肾组织PARP-1表达上调(P＜0.05);与CLP组比较,MB组术后24和48 h时的生存率升高,血清尿素氮、肌酐、NGAL和cystatin C的浓度降低,肾组织PARP-1表达下调(P＜0.05),病理学损伤减轻.结论 亚甲蓝预先给药可减轻脓毒症诱发大鼠急性肾损伤,其机制与下调PARP-1的表达有关.
目的 評價亞甲藍預先給藥對膿毒癥誘髮大鼠急性腎損傷的影響.方法 雄性SD大鼠90隻,1.5 ～ 2.5月齡,體重200～ 250 g,採用隨機數字錶法,將其分為3組(n=30):假手術組(S組)、盲腸結扎穿孔組(CLP組)和亞甲藍組(MB組).S組和CLP組尾靜脈註射生理鹽水0.8 ml;MB組尾靜脈註射10％亞甲藍15 mg/kg(用生理鹽水稀釋至0.8 ml).給藥結束後CLP組和MB組採用盲腸結扎穿孔法製備大鼠膿毒癥模型.每組取20隻大鼠,觀察術後72 h內生存情況.術後18 h時每組處死10隻大鼠,取腎組織,光鏡下觀察病理學結果,分彆採用免疫組化法和Western blot法測定多聚(ADP-覈糖)聚閤酶(PARP-1)的錶達水平;經心髒取血樣,測定血清尿素氮、肌酐、半胱氨痠蛋白酶抑製劑C(cystatin C)和中性粒細胞明膠酶相關載脂蛋白(NGAL)的濃度.結果 與S組比較,CLP組和MB組術後24、48和72 h時的生存率降低,血清尿素氮、肌酐、NGAL和cystatin C的濃度升高,腎組織PARP-1錶達上調(P＜0.05);與CLP組比較,MB組術後24和48 h時的生存率升高,血清尿素氮、肌酐、NGAL和cystatin C的濃度降低,腎組織PARP-1錶達下調(P＜0.05),病理學損傷減輕.結論 亞甲藍預先給藥可減輕膿毒癥誘髮大鼠急性腎損傷,其機製與下調PARP-1的錶達有關.
목적 평개아갑람예선급약대농독증유발대서급성신손상적영향.방법 웅성SD대서90지,1.5 ～ 2.5월령,체중200～ 250 g,채용수궤수자표법,장기분위3조(n=30):가수술조(S조)、맹장결찰천공조(CLP조)화아갑람조(MB조).S조화CLP조미정맥주사생리염수0.8 ml;MB조미정맥주사10％아갑람15 mg/kg(용생리염수희석지0.8 ml).급약결속후CLP조화MB조채용맹장결찰천공법제비대서농독증모형.매조취20지대서,관찰술후72 h내생존정황.술후18 h시매조처사10지대서,취신조직,광경하관찰병이학결과,분별채용면역조화법화Western blot법측정다취(ADP-핵당)취합매(PARP-1)적표체수평;경심장취혈양,측정혈청뇨소담、기항、반광안산단백매억제제C(cystatin C)화중성립세포명효매상관재지단백(NGAL)적농도.결과 여S조비교,CLP조화MB조술후24、48화72 h시적생존솔강저,혈청뇨소담、기항、NGAL화cystatin C적농도승고,신조직PARP-1표체상조(P＜0.05);여CLP조비교,MB조술후24화48 h시적생존솔승고,혈청뇨소담、기항、NGAL화cystatin C적농도강저,신조직PARP-1표체하조(P＜0.05),병이학손상감경.결론 아갑람예선급약가감경농독증유발대서급성신손상,기궤제여하조PARP-1적표체유관.
Objective To evaluate the effect of methylene blue pertreatment on acute kidney injury induced by sepsis in rats.Methods Ninety male Sprague-Dawley rats,aged 1.5-2.5 months,weighing 200-250 g,were randomly assigned into 3 groups (n =30 each) using a random number table:sham operation group (S group),cecal ligation and puncture (CLP) group and methylene blue group (MB group).The rats were anesthetized with 10％ chloral hydrate 350 mg/kg.Normal saline 0.8 ml was injected via the caudal vein in and CLP groups,while 10％ methylene blue 15 mg/kg (in normal saline 0.8 ml) was injected via the caudal vein in group MB.Sepsis was induced by CLP after the end of administration in CLP and MB groups.Twenty animals in each group were chosen and observed for 72 h survival rate.Ten animals were sacrificed in each group at 18 h after operation and kidney specimens were removed for microscopic examination and for determination of the expression of poly (ADP-ribose) polymerase (PARP-1) using immunohistochemistry and Western blot.Blood samples were taken from the heart for determination of serum concentrations of blood urea nitrogen (BUN),creatinine (Cr),cystatin C and neutrophil gelatinase-associated lipocalin (NGAL).Results Compared with group S,the survival rate was significantly decreased at 24,48 and 72 h after operation,and the serum concentrations of BUN,Cr,cystatin C and NGAL and expression of PARP-1 in kidney tissues were increased in CLP and MB groups (P ＜ 0.05).Compared with group CLP,the survival rate was significantly increased at 24 and 48 h after operation,and the serum concentrations of BUN,Cr,cystatin C and NGAL and expression of PARP-1 in kidney tissues were decreased in group MB (P ＜ 0.05).The pathological changes were significantly attenuated in group MB as compared with group CLP.Conclusion Methylene blue pertreatment can attenuate acute kidney injury induced by sepsis in rats through down-regulating the expression of PARP-1.
