CAJ | 학술논문

目的评价七氟醚后处理对大鼠离体心脏缺血再灌注时心肌细胞核糖体40S小亚基蛋白S6激酶(p70S6K)表达的影响.方法取Langendorff心脏模型制备成功的大鼠心脏90个,采用随机数字表法分为3组(n=30):假手术组(S组)、缺血再灌注组(I/R组)和七氟醚后处理组(SP组).离体心脏缺血30 min,再灌注2h.SP组于缺血末至再灌注15 min灌注经3.0％七氟醚饱和的K-H液,随后更换为正常K-H液再灌注105 min.于再灌注2h时测定心肌梗死体积,计算心肌梗死体积百分比,采用Western blot法检测心肌细胞p-p70S6K/t-p70S6K、细胞质和线粒体细胞色素c和caspase-8的表达水平.结果与S组比较,I/R组心肌梗死体积百分比升高,心肌细胞p-p70S6K/t-p70S6K、细胞质细胞色素c和caspase-8表达上调,线粒体细胞色素c表达下调(P＜0.05);与I/R组比较,SP组心肌梗死体积百分比降低,心肌细胞p-p70S6K/t-p70S6K和线粒体细胞色素c表达上调,细胞质细胞色素c和caspase-8表达下调(P＜0.05).结论七氟醚后处理可减轻离体大鼠离体心脏缺血再灌注损伤,其机制与上调心肌细胞p-p70S6K表达,抑制线粒体细胞色素c转移至细胞质,从而减少细胞凋亡有关.
목적 평개칠불미후처리대대서리체심장결혈재관주시심기세포핵당체40S소아기단백S6격매(p70S6K)표체적영향.방법 취Langendorff심장모형제비성공적대서심장90개,채용수궤수자표법분위3조(n=30):가수술조(S조)、결혈재관주조(I/R조)화칠불미후처리조(SP조).리체심장결혈30 min,재관주2h.SP조우결혈말지재관주15 min관주경3.0％칠불미포화적K-H액,수후경환위정상K-H액재관주105 min.우재관주2h시측정심기경사체적,계산심기경사체적백분비,채용Western blot법검측심기세포p-p70S6K/t-p70S6K、세포질화선립체세포색소c화caspase-8적표체수평.결과 여S조비교,I/R조심기경사체적백분비승고,심기세포p-p70S6K/t-p70S6K、세포질세포색소c화caspase-8표체상조,선립체세포색소c표체하조(P＜0.05);여I/R조비교,SP조심기경사체적백분비강저,심기세포p-p70S6K/t-p70S6K화선립체세포색소c표체상조,세포질세포색소c화caspase-8표체하조(P＜0.05).결론 칠불미후처리가감경리체대서리체심장결혈재관주손상,기궤제여상조심기세포p-p70S6K표체,억제선립체세포색소c전이지세포질,종이감소세포조망유관.
Objective To evaluate the effects of sevoflurane postconditioning on the expression of 70 kD aribosomalprotein S6 kinase (p70S6K) during ischemia-reperfusion (I/R) in isolated rat hearts.Methods Pathogen-free male Sprague-Dawley rats,aged 3 months,weighing 270-350 g,were used in the study.Their hearts were excised and perfused in a Langendorff apparatus with K-H solution saturated with 95 ％ O2-5 ％ CO2.Ninety isolated rat hearts with I/R injury were randomly divided into 3 groups (n =30 each):sham operation group (group S),I/R group (group I/R),and sevoflurane postconditioning group (group SP).The hearts were subjected to ischemia for 30 min followed by 2 h reperfusion.In group SP,the hearts were perfused with K-H solution saturated with 3.0％ sevoflurane for 15 min starting from the end of ischemia until 15 min of reperfusion,and then with plain K-H solution for 105 min.At 2 h of reperfusion,myocardial infarct size was measured,the percentage of myocardial infarct size was calculated,and the phosphorylated p70S6K (p-p70S6K)/total p70S6K (tp70S6K) ratio,and cytoplasm,cytochrome C,and caspase-8 expression was measured.Results Compared with group S,the percentage of myocardial infarct size and p-p70S6K/t-p70S6K ratio were significantly increased,the expression of cytochrome C,and caspase-8 was up-regulated,and the expression of cytochrome C was downregulated in I/R group.Compared with I/R group,the percentage of myocardial infarct size was significantly decreased,the ratio of p-p70S6K/t-p70S6K was increased and cytochrome C expression was up-regulated,and the expression of cytochrome C and caspase-8 was down-regulated in SP group.Conclusion Sevoflurane postconditioning can mitigate I/R injury to isolated rat hearts,and up-regulation of p-p70S6K expression,inhibition of transfer of cytochrome C from mitochondria to cytoplasm,and reduced cell apoptosis are involved in the mechanism.