CAJ | 학술논문

目的评价异丙酚对胎鼠离体海马神经元增殖的影响.方法孕16～ 18 d SD大鼠,拉颈处死,剖腹取胎鼠,分离海马神经元.将神经元接种于培养板中,培养至第9天,采用随机数字表法,将其分为7组(n=36):对照组(C组)、脂肪乳剂组(I组)、异丙酚0.1 μmol/L组(P1组)、异丙酚1.0μmol/L组(P2组)、异丙酚10.0 μmol/L组(P3组)、异丙酚100.0 μmol/L组(P4组)和异丙酚1 000.0μmol/L组(P5组).C组不做任何处理;I组培养液中加入10％脂肪乳剂,终浓度为100 μmol/L;P1组、P2组、P3组、P4组和P5组培养液中加入异丙酚,终浓度分别为0.1、1.0、10.0、100.0、1 000.0 μmol/L,继续孵育3h.分别于异丙酚孵育结束后12、24、36、48、60和72 h时采用MTT法测定海马神经元增殖水平.结果与C组比较,P1组、P2组、P3组、P4组和P5组海马神经元增殖水平降低(P＜0.05),I组海马神经元增殖水平差异无统计学意义(P＞0.05);与P1组比较,P2组、P3组和P4组海马神经元增殖水平差异无统计学意义(P＞0.05),P5组海马神经元增殖水平降低(P＜0.05).结论异丙酚可抑制胎鼠离体海马神经元增殖.
목적 평개이병분대태서리체해마신경원증식적영향.방법 잉16～ 18 d SD대서,랍경처사,부복취태서,분리해마신경원.장신경원접충우배양판중,배양지제9천,채용수궤수자표법,장기분위7조(n=36):대조조(C조)、지방유제조(I조)、이병분0.1 μmol/L조(P1조)、이병분1.0μmol/L조(P2조)、이병분10.0 μmol/L조(P3조)、이병분100.0 μmol/L조(P4조)화이병분1 000.0μmol/L조(P5조).C조불주임하처리;I조배양액중가입10％지방유제,종농도위100 μmol/L;P1조、P2조、P3조、P4조화P5조배양액중가입이병분,종농도분별위0.1、1.0、10.0、100.0、1 000.0 μmol/L,계속부육3h.분별우이병분부육결속후12、24、36、48、60화72 h시채용MTT법측정해마신경원증식수평.결과 여C조비교,P1조、P2조、P3조、P4조화P5조해마신경원증식수평강저(P＜0.05),I조해마신경원증식수평차이무통계학의의(P＞0.05);여P1조비교,P2조、P3조화P4조해마신경원증식수평차이무통계학의의(P＞0.05),P5조해마신경원증식수평강저(P＜0.05).결론 이병분가억제태서리체해마신경원증식.
Objective To evaluate the effects of propofol on the proliferation of hippocampal neurons in fetal rats in vitro.Methods Pregnant Sprague-Dawley rats at 16-18 days of gestation,were sacrificed and the fetal rats were taken out from the abdominal cavity.The hippocampal neurons of the fetal rats were isolated and seeded in culture plates.After being cultured for 9 days,the neurons were divided into 7 groups using a random number table(n =36 each):control group (C group),intralipid group (I group) and propofol 0.1,1.0,10.0,100.0,1 000.0 μmol/L groups (P1-5 groups).In group I,10％ intralipid was added to the culture media until the final concentration reached 100 μmol/L.In P1-5 groups,propofol was added to the culture media until the final concentration reached 0.1,1.0,10.0,100.0 and 1 000.0 μmol/L,respectively.The neurons were then incubated for 3 h.The proliferation of hippocampal neurons was determined by MTT assay at 12,24,36,48,60 and 72 h after the end of incubation with propofol.Results Compared with group C,the proliferation of hippocampal neurons was significantly decreased in P1-5 groups (P ＜ 0.05),while no significant change was found in group I (P ＞ 0.05).Compared with group P1,the proliferation of hippocampal neurons was significantly decreased in group P5 (P ＜ 0.05),while no significant change was found in P2-4 groups (P ＞ 0.05).Conclusion Propofol can decrease the proliferation of hippocampal neurons in fetal rats in vitro.