中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
5期
581-583
,共3页
贺俭%王汉兵%何万友%廖美娟%王靖%杨承祥
賀儉%王漢兵%何萬友%廖美娟%王靖%楊承祥
하검%왕한병%하만우%료미연%왕정%양승상
糖尿病%神经痛%自噬%脊髓%神经元
糖尿病%神經痛%自噬%脊髓%神經元
당뇨병%신경통%자서%척수%신경원
Diabetes mellitus%Neuralgia%Autophagy%Spinal cord%Neurons
目的 评价糖尿病神经痛大鼠脊髓神经元自噬的变化.方法 成年雄性SD大鼠48只,体重220 ～ 250 g,采用随机数字表法,将其分为2组:对照组(C组,n=8)和糖尿病神经痛组(DNP组,n=40).腹腔注射链脲佐菌素(STZ) 60 mg/kg建立大鼠糖尿病模型.DNP组分别于造模前1d和造模后1、2、4、8周各取8只大鼠,C组于相应时点,测定机械痛阈.DNP组于上述时点痛阈测定结束后,处死大鼠,取脊髓组织,采用Western blot法测定LC3-Ⅰ、LC3-Ⅱ、Beclin-1和p62蛋白的表达,计算LC3-Ⅱ/LC3-Ⅰ比值.结果 与C组比较,DNP组造模后2、4和8周时机械痛阈降低(P＜0.05).与造模前比较,DNP组造模后2、4和8周时脊髓LC3-Ⅱ和Beclin-1表达上调,p62蛋白表达下调,LC3-Ⅱ/LC3-Ⅰ比值升高(P＜0.05).结论 脊髓神经元自噬增强可能参与了大鼠糖尿病神经痛的形成和维持.
目的 評價糖尿病神經痛大鼠脊髓神經元自噬的變化.方法 成年雄性SD大鼠48隻,體重220 ～ 250 g,採用隨機數字錶法,將其分為2組:對照組(C組,n=8)和糖尿病神經痛組(DNP組,n=40).腹腔註射鏈脲佐菌素(STZ) 60 mg/kg建立大鼠糖尿病模型.DNP組分彆于造模前1d和造模後1、2、4、8週各取8隻大鼠,C組于相應時點,測定機械痛閾.DNP組于上述時點痛閾測定結束後,處死大鼠,取脊髓組織,採用Western blot法測定LC3-Ⅰ、LC3-Ⅱ、Beclin-1和p62蛋白的錶達,計算LC3-Ⅱ/LC3-Ⅰ比值.結果 與C組比較,DNP組造模後2、4和8週時機械痛閾降低(P＜0.05).與造模前比較,DNP組造模後2、4和8週時脊髓LC3-Ⅱ和Beclin-1錶達上調,p62蛋白錶達下調,LC3-Ⅱ/LC3-Ⅰ比值升高(P＜0.05).結論 脊髓神經元自噬增彊可能參與瞭大鼠糖尿病神經痛的形成和維持.
목적 평개당뇨병신경통대서척수신경원자서적변화.방법 성년웅성SD대서48지,체중220 ～ 250 g,채용수궤수자표법,장기분위2조:대조조(C조,n=8)화당뇨병신경통조(DNP조,n=40).복강주사련뇨좌균소(STZ) 60 mg/kg건립대서당뇨병모형.DNP조분별우조모전1d화조모후1、2、4、8주각취8지대서,C조우상응시점,측정궤계통역.DNP조우상술시점통역측정결속후,처사대서,취척수조직,채용Western blot법측정LC3-Ⅰ、LC3-Ⅱ、Beclin-1화p62단백적표체,계산LC3-Ⅱ/LC3-Ⅰ비치.결과 여C조비교,DNP조조모후2、4화8주시궤계통역강저(P＜0.05).여조모전비교,DNP조조모후2、4화8주시척수LC3-Ⅱ화Beclin-1표체상조,p62단백표체하조,LC3-Ⅱ/LC3-Ⅰ비치승고(P＜0.05).결론 척수신경원자서증강가능삼여료대서당뇨병신경통적형성화유지.
Objective To evaluate the changes in autophagy in spinal neurons of rats with diabetic neuropathic pain (DNP).Methods Forty-eight male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into control group (group C,n =8) and group DNP (n =40) using a random number table.Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ) 60 mg/kg.Before STZ injection and at 1,2,4,and 8 weeks after STZ injection,8 rats randomly chosen from each group were used to measure mechanical pain threshold.The animals were sacrificed after measurement of mechanical pain threshold and the spinal cords were removed for determination of the expression of LC3-Ⅰ,LC3-Ⅱ,Beclin-1 and p62 protein.LC3-Ⅱ/LC3-Ⅰ ratio was calculated.Results Compared with group C,mechanical pain threshold was significantly decreased at 2,4,and 8 weeks after STZ injection in group DNP.The expression of LC3-Ⅱ and Beclin-1 in the spinal cord was significantly up-regulated,p62 protein expression was down-regulated,and LC3-Ⅱ/LC3-Ⅰ ratio was increased at 2,4,and 8 weeks after STZ injection as compared with the baseline value before STZ injection in group DNP.Conclusion Enhanced autophagy in spinal neurons may be involved in the development and maintenance of DNP in rats.
