中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
5期
624-626
,共3页
杨威%许德奖%赵刚%赵国栋
楊威%許德獎%趙剛%趙國棟
양위%허덕장%조강%조국동
胰蛋白酶抑制剂%胃溃疡%应激%T淋巴细胞,辅助诱导
胰蛋白酶抑製劑%胃潰瘍%應激%T淋巴細胞,輔助誘導
이단백매억제제%위궤양%응격%T림파세포,보조유도
Trypsin inhibitors%Stomach ulcer%Stress%T-Lymphocytes,helper-inducer
目的 评价乌司他丁对应激性胃溃疡大鼠Th1/Th2细胞平衡的影响.方法 SPF级雄性SD大鼠24只,6～8周龄,体重180 ～ 220 g,采用随机数字表法分为3组(n=8):对照组(C组)、应激性胃溃疡组(GU组)和乌司他丁组(U组).采用束缚浸水应激3.5h的方法制备大鼠应激性胃溃疡模型,浸水前10 min,GU组经尾静脉注射生理盐水0.4ml,U组经尾静脉注射乌司他丁10万U/kg.于模型制备完成后即刻,麻醉下开腹,显微镜下评价胃粘膜损伤程度,记录胃溃疡指数(UI),并观察胃粘膜病理学结果;取脾组织,采用PCR法测定脾组织T-bet、Gata-3、干扰素-γ(IFN-γ)、IL-4的mRNA表达.结果 与C组比较,GU组T-bet mRNA表达下调,Gata-3 mRNA表达上调,T-bet/Gata-3比值降低,UI升高(P＜0.05),IFN-γ和IL-4的mRNA表达差异无统计学意义(P＞0.05),U组UI升高(P＜0.05),其余各指标差异无统计学意义(P＞0.05).与GU组比较,U组T-bet/Gata-3比值升高,UI降低(P＜0.05),T-bet、Gata-3、IFN-γ、IL-4的mRNA表达差异无统计学意义(P＞0.05).U组胃粘膜病理学损伤较GU组减轻.结论 乌司他丁减轻大鼠应激性胃溃疡的机制可能与纠正Th1/Th2细胞失衡状态有关.
目的 評價烏司他丁對應激性胃潰瘍大鼠Th1/Th2細胞平衡的影響.方法 SPF級雄性SD大鼠24隻,6～8週齡,體重180 ～ 220 g,採用隨機數字錶法分為3組(n=8):對照組(C組)、應激性胃潰瘍組(GU組)和烏司他丁組(U組).採用束縳浸水應激3.5h的方法製備大鼠應激性胃潰瘍模型,浸水前10 min,GU組經尾靜脈註射生理鹽水0.4ml,U組經尾靜脈註射烏司他丁10萬U/kg.于模型製備完成後即刻,痳醉下開腹,顯微鏡下評價胃粘膜損傷程度,記錄胃潰瘍指數(UI),併觀察胃粘膜病理學結果;取脾組織,採用PCR法測定脾組織T-bet、Gata-3、榦擾素-γ(IFN-γ)、IL-4的mRNA錶達.結果 與C組比較,GU組T-bet mRNA錶達下調,Gata-3 mRNA錶達上調,T-bet/Gata-3比值降低,UI升高(P＜0.05),IFN-γ和IL-4的mRNA錶達差異無統計學意義(P＞0.05),U組UI升高(P＜0.05),其餘各指標差異無統計學意義(P＞0.05).與GU組比較,U組T-bet/Gata-3比值升高,UI降低(P＜0.05),T-bet、Gata-3、IFN-γ、IL-4的mRNA錶達差異無統計學意義(P＞0.05).U組胃粘膜病理學損傷較GU組減輕.結論 烏司他丁減輕大鼠應激性胃潰瘍的機製可能與糾正Th1/Th2細胞失衡狀態有關.
목적 평개오사타정대응격성위궤양대서Th1/Th2세포평형적영향.방법 SPF급웅성SD대서24지,6～8주령,체중180 ～ 220 g,채용수궤수자표법분위3조(n=8):대조조(C조)、응격성위궤양조(GU조)화오사타정조(U조).채용속박침수응격3.5h적방법제비대서응격성위궤양모형,침수전10 min,GU조경미정맥주사생리염수0.4ml,U조경미정맥주사오사타정10만U/kg.우모형제비완성후즉각,마취하개복,현미경하평개위점막손상정도,기록위궤양지수(UI),병관찰위점막병이학결과;취비조직,채용PCR법측정비조직T-bet、Gata-3、간우소-γ(IFN-γ)、IL-4적mRNA표체.결과 여C조비교,GU조T-bet mRNA표체하조,Gata-3 mRNA표체상조,T-bet/Gata-3비치강저,UI승고(P＜0.05),IFN-γ화IL-4적mRNA표체차이무통계학의의(P＞0.05),U조UI승고(P＜0.05),기여각지표차이무통계학의의(P＞0.05).여GU조비교,U조T-bet/Gata-3비치승고,UI강저(P＜0.05),T-bet、Gata-3、IFN-γ、IL-4적mRNA표체차이무통계학의의(P＞0.05).U조위점막병이학손상교GU조감경.결론 오사타정감경대서응격성위궤양적궤제가능여규정Th1/Th2세포실형상태유관.
Objective To evaluate the effect of ulinastatin on Th1/Th2 cell balance in a rat model of stress-induced gastric ulcer (GU).Methods Twenty-four male SPF Sprague-Dawley rats,aged 6-8 weeks,weighing 180-220 g,were randomly divided into 3 groups (n =8 each) using a random number table:control group (group C),stress-induced GU group (group GU) and ulinastatin group (group U).The rats were subjected to water-immersion and restraint stress for 3.5 h to establish GU model in S and U groups.At 10 min before water-immersion,normal saline 0.4 ml and ulinastatin 100 000 U/kg were injected via the tail vein in GU and U groups,respectively.After GU model was established,the abdomen was opened after anesthesia.The whole stomach was exposed,and the gastric mucosal damage was evaluated under microscope,ulcer index (UI) was recorded,and the pathological changes of the gastric mucous membrane were observed.Spleen samples were obtained to detect T-bet,Gata-3,interferon-γ (IFN-γ),and interleukin-4 (IL-4) mRNA expression by RT-PCR.The ratio of T-bet mRNA expression/Gata-3 mRNA expression (T-bet/Gata-3) was calculated.Results Compared with group C,T-bet mRNA expression was significantly down-regulated,Gata-3 mRNA expression was up-regulated,T-bet/Gata-3 ratio was decreased,UI was increased,and no significant changes were found in IFN-γ and IL-4 mRNA expression in GU group,and UI was increased,and no significant changes were found in the other parameters in group U.Compared with group GU,the ratio of T-bet/Gata-3 was significantly increased,and UI was decreased,and no significant changes were found in T-bet,Gata-3,IFN-γ,IL-4 mRNA expression in group U.The gastric mucosal damage was significantly mitigated in GU group as compared with group U.Conclusion The mechanism by which ulinastatin mitigates stress-induced GU may be related to correction of Th1/Th2 cell imbalance in rats.
