中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
5期
634-636
,共3页
Toll样受体4%舒芬太尼%心肌再灌注损伤%炎症
Toll樣受體4%舒芬太尼%心肌再灌註損傷%炎癥
Toll양수체4%서분태니%심기재관주손상%염증
Toll-like receptor 4%Sufentanil%Myocardial reperfusion injury%Inflammation
目的 评价Toll样受体4(TLR4)在舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤后炎性反应中的作用.方法 清洁级健康成年雄性Wistar大鼠30只,体重250～300 g,采用随机数字表法分为3组(n=10):假手术组(S组);缺血再灌注组(IR组)采用结扎大鼠心脏左冠状动脉前降支30min恢复灌注的方法制备心肌缺血再灌注损伤模型;舒芬太尼后处理组(SP组)缺血30 min,于再灌注前5 min静脉注射舒芬太尼0.6μg/kg.于再灌注120 min时取腹主动脉血及心肌标本,采用ELISA法测定血清IL-6和TNF-α浓度及心肌TLR4含量.结果 与S组比较,其余2组血清IL-6和TNF-α浓度及心肌TLR4含量升高(P＜0.05);与IR组比较,SP组血清IL-6和TNF-α浓度及心肌TLR4含量降低(P＜0.05).结论 舒芬太尼后处理可减轻大鼠心肌缺血再灌注损伤后炎性反应,其机制可能与下调心肌组织TLR4表达有关.
目的 評價Toll樣受體4(TLR4)在舒芬太尼後處理減輕大鼠心肌缺血再灌註損傷後炎性反應中的作用.方法 清潔級健康成年雄性Wistar大鼠30隻,體重250～300 g,採用隨機數字錶法分為3組(n=10):假手術組(S組);缺血再灌註組(IR組)採用結扎大鼠心髒左冠狀動脈前降支30min恢複灌註的方法製備心肌缺血再灌註損傷模型;舒芬太尼後處理組(SP組)缺血30 min,于再灌註前5 min靜脈註射舒芬太尼0.6μg/kg.于再灌註120 min時取腹主動脈血及心肌標本,採用ELISA法測定血清IL-6和TNF-α濃度及心肌TLR4含量.結果 與S組比較,其餘2組血清IL-6和TNF-α濃度及心肌TLR4含量升高(P＜0.05);與IR組比較,SP組血清IL-6和TNF-α濃度及心肌TLR4含量降低(P＜0.05).結論 舒芬太尼後處理可減輕大鼠心肌缺血再灌註損傷後炎性反應,其機製可能與下調心肌組織TLR4錶達有關.
목적 평개Toll양수체4(TLR4)재서분태니후처리감경대서심기결혈재관주손상후염성반응중적작용.방법 청길급건강성년웅성Wistar대서30지,체중250～300 g,채용수궤수자표법분위3조(n=10):가수술조(S조);결혈재관주조(IR조)채용결찰대서심장좌관상동맥전강지30min회복관주적방법제비심기결혈재관주손상모형;서분태니후처리조(SP조)결혈30 min,우재관주전5 min정맥주사서분태니0.6μg/kg.우재관주120 min시취복주동맥혈급심기표본,채용ELISA법측정혈청IL-6화TNF-α농도급심기TLR4함량.결과 여S조비교,기여2조혈청IL-6화TNF-α농도급심기TLR4함량승고(P＜0.05);여IR조비교,SP조혈청IL-6화TNF-α농도급심기TLR4함량강저(P＜0.05).결론 서분태니후처리가감경대서심기결혈재관주손상후염성반응,기궤제가능여하조심기조직TLR4표체유관.
Objective To evaluate the role of Toll-like receptor 4 (TLR4) in mitigation of inflammatory responses following myocardial ischemia-reperfusion (I/R) injury by sufentanil postconditioning in rats.Methods Thirty Wistar rats were randomly divided into 3 groups (n =10 each):sham operation group (S group); I/R group; sufentanil postconditioning group (SP group).Myocardial I/R was produced by temporary ligation of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.In group SP,sufentanil 0.6 μg/kg was injected intravenously at 5 min before reperfusion.The myocardial specimens and blood samples were taken at the end of 120 min reperfusion for determination of the interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations and TLR4 content (by ELISA).Results Compared with S group,the levels of TLR4,IL-6 and TNF-α were significantly increased in the other two groups.Comparedwith I/R group,the levels of TLR4,IL-6 and TNF-α were significantly decreased in group SP.Conclusion The mechanism by which sufentanil postconditioning mitigates inflammatory responses following myocardial I/R injury may be related to downregulation of TLR4 expression in myocardial tissues of rats.
