中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
6期
677-678
,共2页
依托咪酯%缺血预处理%药物毒性%肾上腺皮质
依託咪酯%缺血預處理%藥物毒性%腎上腺皮質
의탁미지%결혈예처리%약물독성%신상선피질
Etomidate%Ischemic preconditioning%Drug toxicity%Adrenal cortex
目的 评价依托咪酯预处理对依托咪酯诱导大鼠肾上腺皮质细胞毒性的影响.方法 选择原代培养7～9d指数生长期的大鼠肾上腺皮质细胞,接种于96孔板上继续培养24h,密度为1×106个/ml,采用随机数字表法,将其分为3组:对照组(C组)、依托咪酯组(E组)和依托咪酯预处理组(EP组),每组6孔.C组不给予任何处理;E组经依托咪酯700 μmol/L孵育24h;EP组先经依托咪酯1.25 μnol/L孵育1h,洗脱后,再经依托咪酯700 μmol/L孵育24h.采用CCK-8染色法检测细胞活力,采用ELISA染色法检测皮质醇浓度.结果 与C组比较,E组和EP组细胞活力和皮质醇浓度降低(P＜0.05);与E组比较,EP组细胞活力和皮质醇浓度升高(P＜0.05).结论 依托咪酯预处理可减轻依托咪酯对大鼠肾上腺皮质细胞的毒性作用.
目的 評價依託咪酯預處理對依託咪酯誘導大鼠腎上腺皮質細胞毒性的影響.方法 選擇原代培養7～9d指數生長期的大鼠腎上腺皮質細胞,接種于96孔闆上繼續培養24h,密度為1×106箇/ml,採用隨機數字錶法,將其分為3組:對照組(C組)、依託咪酯組(E組)和依託咪酯預處理組(EP組),每組6孔.C組不給予任何處理;E組經依託咪酯700 μmol/L孵育24h;EP組先經依託咪酯1.25 μnol/L孵育1h,洗脫後,再經依託咪酯700 μmol/L孵育24h.採用CCK-8染色法檢測細胞活力,採用ELISA染色法檢測皮質醇濃度.結果 與C組比較,E組和EP組細胞活力和皮質醇濃度降低(P＜0.05);與E組比較,EP組細胞活力和皮質醇濃度升高(P＜0.05).結論 依託咪酯預處理可減輕依託咪酯對大鼠腎上腺皮質細胞的毒性作用.
목적 평개의탁미지예처리대의탁미지유도대서신상선피질세포독성적영향.방법 선택원대배양7～9d지수생장기적대서신상선피질세포,접충우96공판상계속배양24h,밀도위1×106개/ml,채용수궤수자표법,장기분위3조:대조조(C조)、의탁미지조(E조)화의탁미지예처리조(EP조),매조6공.C조불급여임하처리;E조경의탁미지700 μmol/L부육24h;EP조선경의탁미지1.25 μnol/L부육1h,세탈후,재경의탁미지700 μmol/L부육24h.채용CCK-8염색법검측세포활력,채용ELISA염색법검측피질순농도.결과 여C조비교,E조화EP조세포활력화피질순농도강저(P＜0.05);여E조비교,EP조세포활력화피질순농도승고(P＜0.05).결론 의탁미지예처리가감경의탁미지대대서신상선피질세포적독성작용.
Objective To evaluate the effects of etomidate preconditioning on etomidate-induced toxicity to rat adrenal cortical cells in vitro.Methods After being primarily cultured for 7-9 days,the rat adrenal cortical cells at the exponential growth phase were seeded into 96-well culture plates (1 × 106 cells/ml) and cultured for 24 h.The cells were then randomly divided into 3 groups with 6 wells in each group:control group (group C),etomidate group (group E),and etomidate preconditioning group (group EP).In group E,the cells were incubated with 700 μmol/L etomidate for 24 h.In group EP,the cells were incubated with 1.25 μmol/L etomidate for 1 h,then washed out and incubated with 700 μmol/L eomidate for 24 h.The cell viability was determined by CCK-8 assay and the concentration of cortisol was determined by ELISA.Results Compared with group C,the cell viability and cortisol concentration were significantly decreased in E and EP groups.Compared with group E,the cell viability and cortisol concentration were significantly increased in group EP.Conclusion Etomidate preconditioning can reduce etomidate-induced toxicity to rat adrenal cortical cells in vitro.
