CAJ | 학술논문

目的评价右美托咪定对神经病理性痛大鼠脊髓基质金属蛋白酶9(MMP-9)表达的影响.方法清洁级健康成年雄性SD大鼠81只,体重190 ～ 230 g,采用随机数字表法分为3组(n=27):假手术组(S组)仅分离坐骨神经但不结扎;神经病理性痛组(NP组)采用结扎坐骨神经的方法制备大鼠神经病理性痛模型;右美托咪定组(Dex组)于结扎坐骨神经后至处死每天腹腔注射右美托咪定50 μg/kg,S组和NP组注射等容量生理盐水.于结扎前1d、结扎后5、9和16 d(T0-3)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL);于T1-3时测定痛阈后各组随机取9只大鼠处死,取L4.5脊髓,采用免疫组化法检测MMP-9表达,ELISA法测定TNF-α含量.结果与S组比较,NP组和Dex组T1-3时MWT降低,TWL缩短,脊髓MMP-9和TNF-α水平升高(P＜0.05);与NP组比较,Dex组T1-3时MWT升高,TWL延长,脊髓MMP-9和TNF-α水平降低(P＜0.05).结论右美托咪定可抑制脊髓MMP-9表达上调,降低炎性反应,从而减轻大鼠神经病理性痛.
목적 평개우미탁미정대신경병이성통대서척수기질금속단백매9(MMP-9)표체적영향.방법 청길급건강성년웅성SD대서81지,체중190 ～ 230 g,채용수궤수자표법분위3조(n=27):가수술조(S조)부분리좌골신경단불결찰;신경병이성통조(NP조)채용결찰좌골신경적방법제비대서신경병이성통모형;우미탁미정조(Dex조)우결찰좌골신경후지처사매천복강주사우미탁미정50 μg/kg,S조화NP조주사등용량생리염수.우결찰전1d、결찰후5、9화16 d(T0-3)시측정궤계축족반응역(MWT)화열축족잠복기(TWL);우T1-3시측정통역후각조수궤취9지대서처사,취L4.5척수,채용면역조화법검측MMP-9표체,ELISA법측정TNF-α함량.결과 여S조비교,NP조화Dex조T1-3시MWT강저,TWL축단,척수MMP-9화TNF-α수평승고(P＜0.05);여NP조비교,Dex조T1-3시MWT승고,TWL연장,척수MMP-9화TNF-α수평강저(P＜0.05).결론 우미탁미정가억제척수MMP-9표체상조,강저염성반응,종이감경대서신경병이성통.
Objective To evaluate the effects of dexmedetomidine on the expression of spinal matrix metalloproteinase-9 (MMP-9) in a rat model of neuropathic pain (NP).Methods Eighty-one adult male SpragueDawley rats,weighing 190-230 g,were randomly divided into 3 groups (n =27 each) using a random number table:sham operation group (group S); group NP; dexmedetomidine group (group Dex).The animals were anesthetized with intraperitoneal 10％ chloral hydrate 350 mg/kg.The right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread in NP and Dex groups.In group Dex,dexmedetomidine 50 μg/kg was injected intraperitoneally once a day starting from the end of operation until the animals were sacrificed.The equal volume of normal saline was given instead of dexmedetomidine in S and NP groups.Paw withdrawal threshold to von Frey filament stimulation (MWT) and paw withdrawal latency to thermal stimulation (TWL) were measured at 1 day before operation (To,baseline) and 5,9 and 16 days after operation (T1-3).Nine animals were sacrificed after measurement of pain threshold at T1-3 and their lumbar segments (L4,5) of the spinal cord were removed for detection of MMP-9 expression (by immuno-histochemistry) and tumor necrosis factor-alpha (TNF-α) content (by ELISA).Results Compared with group S,MWT was significantly decreased,TWL was shortened,and the levels of MMP-9 and TNF-α were increased at T1-3 in NP and Dex groups.Compared with NP group,MWT was significantly increased,TWL was prolonged,and the levels of MMP-9 and TNF-α were decreased at T1-3 in Dex group.Conclusion Dexmedetomidine can inhibit up-regulation of MMP-9 expression,and decrease inflammatory responses,thus attenuating NP in rats.