中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
6期
743-745
,共3页
杨磊%莫绪明%尹宁%范欢欢
楊磊%莫緒明%尹寧%範歡歡
양뢰%막서명%윤저%범환환
磷酸二酯酶抑制剂%高血压,肺性%肿瘤坏死因子α
燐痠二酯酶抑製劑%高血壓,肺性%腫瘤壞死因子α
린산이지매억제제%고혈압,폐성%종류배사인자α
Phosphodiesterase inhibitors%Hypertension,pulmonary%Tumor necrosis factor-alpha
目的 评价西地那非对肺动脉高压大鼠肺组织TNF-α表达的影响.方法 健康雄性SD大鼠24只,8周龄,体重180 ～ 220 g,采用随机数字表法,将其分为3组(n=8):对照组(C组)、肺动脉高压组(PH组)、西地那非组(S组).S组左肺切除术后1d,应用西地那非50 mg/kg灌胃,1次/d,连续35 d.PH组和S组左肺切除术后7d背部皮下注射野百合碱建立肺动脉高压模型.左肺切除术后35 d测定平均肺动脉压(mPAP)、肺小动脉相对中膜厚度(RMT)、右室收缩压(RVSP)和肺动脉肌化程度,计算右心室重量与左心室和室间隔重量比值[RV/(LV+S)比值];采用实时荧光RT-PCR定量法和Western blot法分别测定肺组织TNF-α mRNA和蛋白表达.结果 与C组相比,PH组大鼠mPAP、RVSP、肺动脉肌化程度、RMT和RV/(LV+S)比值升高,肺组织TNF-α mRNA和蛋白表达水平上调,S组大鼠RVSP、肺动脉肌化程度和RV/(LV+S)比值升高(P＜0.05);与PH组比较,S组大鼠mPAP、RVSP、肺动脉肌化程度、RMT和RV/(LV+S)比值降低,肺组织TNF-α mRNA和蛋白表达水平下调(P＜0.05).结论 西地那非可下调肺动脉高压大鼠肺组织TNF-α的表达,抑制肺动脉重构,降低肺动脉压.
目的 評價西地那非對肺動脈高壓大鼠肺組織TNF-α錶達的影響.方法 健康雄性SD大鼠24隻,8週齡,體重180 ～ 220 g,採用隨機數字錶法,將其分為3組(n=8):對照組(C組)、肺動脈高壓組(PH組)、西地那非組(S組).S組左肺切除術後1d,應用西地那非50 mg/kg灌胃,1次/d,連續35 d.PH組和S組左肺切除術後7d揹部皮下註射野百閤堿建立肺動脈高壓模型.左肺切除術後35 d測定平均肺動脈壓(mPAP)、肺小動脈相對中膜厚度(RMT)、右室收縮壓(RVSP)和肺動脈肌化程度,計算右心室重量與左心室和室間隔重量比值[RV/(LV+S)比值];採用實時熒光RT-PCR定量法和Western blot法分彆測定肺組織TNF-α mRNA和蛋白錶達.結果 與C組相比,PH組大鼠mPAP、RVSP、肺動脈肌化程度、RMT和RV/(LV+S)比值升高,肺組織TNF-α mRNA和蛋白錶達水平上調,S組大鼠RVSP、肺動脈肌化程度和RV/(LV+S)比值升高(P＜0.05);與PH組比較,S組大鼠mPAP、RVSP、肺動脈肌化程度、RMT和RV/(LV+S)比值降低,肺組織TNF-α mRNA和蛋白錶達水平下調(P＜0.05).結論 西地那非可下調肺動脈高壓大鼠肺組織TNF-α的錶達,抑製肺動脈重構,降低肺動脈壓.
목적 평개서지나비대폐동맥고압대서폐조직TNF-α표체적영향.방법 건강웅성SD대서24지,8주령,체중180 ～ 220 g,채용수궤수자표법,장기분위3조(n=8):대조조(C조)、폐동맥고압조(PH조)、서지나비조(S조).S조좌폐절제술후1d,응용서지나비50 mg/kg관위,1차/d,련속35 d.PH조화S조좌폐절제술후7d배부피하주사야백합감건립폐동맥고압모형.좌폐절제술후35 d측정평균폐동맥압(mPAP)、폐소동맥상대중막후도(RMT)、우실수축압(RVSP)화폐동맥기화정도,계산우심실중량여좌심실화실간격중량비치[RV/(LV+S)비치];채용실시형광RT-PCR정량법화Western blot법분별측정폐조직TNF-α mRNA화단백표체.결과 여C조상비,PH조대서mPAP、RVSP、폐동맥기화정도、RMT화RV/(LV+S)비치승고,폐조직TNF-α mRNA화단백표체수평상조,S조대서RVSP、폐동맥기화정도화RV/(LV+S)비치승고(P＜0.05);여PH조비교,S조대서mPAP、RVSP、폐동맥기화정도、RMT화RV/(LV+S)비치강저,폐조직TNF-α mRNA화단백표체수평하조(P＜0.05).결론 서지나비가하조폐동맥고압대서폐조직TNF-α적표체,억제폐동맥중구,강저폐동맥압.
Objective To evaluate the effect of sildenafil on the expression of tumor necrosis factor-alpha (TNF-α) in lung tissues of rats with pulmonary hypertension.Methods Twenty-four male Sprague-Dawley rats,aged 8 weeks,weighing 180-220 g,were randomly divided into 3 groups (n =8 each) using a random number table:control group (group C),pulmonary hypertension group (group PH),and sildenafil group (group S).Sildenafil 50 mg/kg was injected through a gastric tube into stomach once a day for 35 consecutive days starting from 1 day after lelf pneumonectomy in group S.Pulmonary hypertension was induced by left pneumonectomy and subcutaneous monocrotaline injected at 7 days after operation in PH and S groups.At 35 days after operation,mean pulmonary arterial pressure (mPAP),relative medial thickness of pulmonary artery (RMT),right ventricular systolic pressure (RVSP),and muscularization of small pulmonary arteries were measured in the lung.The ratio of the right ventricular weight to the sum of the weights of the left ventricle and septum (RV/(LV + S)) was calculated.The expression of TNF-α mRNA and protein was determined using RT-PCR and Western blot analysis,respectively.Results Compared with group C,mPAP,RVSP,muscularization of small pulmonary arteries,RMT and RV/(LV + S) ratio were significantly increased,and the expression of TNF-α mRNA and protein was upregulated in group PH,and RVSP,muscularization of small pulmonary arteries and RV/(LV + S) ratio were increased in group S.Compared with group PH,mPAP,RVSP,muscularization of small pulmonary arteries,RMT and RV/(LV + S) ratio were significantly decreased,and the expression of TNF-α mRNA and protein was downregulated in group S.Conclusion Sildenafil can down-regulate the expression of TNF-α in lung tissues of rats with pulmonary hypertension,inhibit reconstruction of pulmonary artery,and decrease the pulmonary arterial pressure.
