目的 评价姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响.方法 选择SPF级雄性Wistar-Kyoto大鼠48只,8周龄,体重180 ～ 200 g,采用随机数字表法,将其分为2组(n=24):假手术组(W-Sham组)、缺血再灌注组(W-1/R组).SPF级雄性自发性高血压(SH)大鼠72只,8周龄,体重180 ～ 200 g,采用随机数字表法,将其分为3组(n=24):假手术组(S-Sham组)、缺血再灌注组(S-I/R组)、姜黄素组(S-Cur组).采用四血管阻断法建立全脑缺血再灌注损伤模型(缺血10min后恢复灌注).S-Cur组于再灌注30 min时腹腔注射姜黄素100 mg/kg,W-Sham组、S-Sham组、W-I/R组和S-I/R组于再灌注30 min时腹腔注射玉米油5ml/kg.于再灌注7d时行跳台实验测定学习和记忆成绩,于再灌注3h、1、3和7 d(T1-4)时处死取海马组织,苏木素-伊红染色后观察海马CA1区锥体细胞形态,Nissl染色后计数海马CA1区平均锥体细胞密度,ELISA法测定海马IL-1β、TNF-α和IL-10的含量.结果 与W-Sham组比较,W-I/R组学习和记忆成绩降低,海马CA1区平均锥体细胞密度减少,T1-3时海马IL-1β含量升高,T1时海马TNF-α含量升高,海马IL-10含量于T2时升高,T1,3,4时降低(P＜0.05),S-Sham组上述各指标比较差异无统计学意义(P＞0.05);与W-I/R组比较,S-I/R组学习和记忆成绩降低,T1.3时海马IL-1β含量降低,T1时海马TNF-α含量升高,T2时海马IL-10含量降低(P＜0.05),海马CA1区平均锥体细胞密度差异无统计学意义(P＞0.05),海马CA1区锥体细胞损伤严重;与S-I/R组比较,S-Gur组学习和记忆成绩升高,海马CA1区平均锥体细胞密度增多,T2,3时海马IL-1β含量降低,T1-4时海马TNF-α含量降低,T2时海马IL-10含量升高(P＜0.05),海马CA1区锥体细胞损伤减轻.结论 姜黄素减轻高血压病大鼠全脑缺血再灌注损伤的机制可能与抑制海马炎性反应有关.
目的 評價薑黃素對高血壓病大鼠全腦缺血再灌註損傷時海馬炎性反應的影響.方法 選擇SPF級雄性Wistar-Kyoto大鼠48隻,8週齡,體重180 ～ 200 g,採用隨機數字錶法,將其分為2組(n=24):假手術組(W-Sham組)、缺血再灌註組(W-1/R組).SPF級雄性自髮性高血壓(SH)大鼠72隻,8週齡,體重180 ～ 200 g,採用隨機數字錶法,將其分為3組(n=24):假手術組(S-Sham組)、缺血再灌註組(S-I/R組)、薑黃素組(S-Cur組).採用四血管阻斷法建立全腦缺血再灌註損傷模型(缺血10min後恢複灌註).S-Cur組于再灌註30 min時腹腔註射薑黃素100 mg/kg,W-Sham組、S-Sham組、W-I/R組和S-I/R組于再灌註30 min時腹腔註射玉米油5ml/kg.于再灌註7d時行跳檯實驗測定學習和記憶成績,于再灌註3h、1、3和7 d(T1-4)時處死取海馬組織,囌木素-伊紅染色後觀察海馬CA1區錐體細胞形態,Nissl染色後計數海馬CA1區平均錐體細胞密度,ELISA法測定海馬IL-1β、TNF-α和IL-10的含量.結果 與W-Sham組比較,W-I/R組學習和記憶成績降低,海馬CA1區平均錐體細胞密度減少,T1-3時海馬IL-1β含量升高,T1時海馬TNF-α含量升高,海馬IL-10含量于T2時升高,T1,3,4時降低(P＜0.05),S-Sham組上述各指標比較差異無統計學意義(P＞0.05);與W-I/R組比較,S-I/R組學習和記憶成績降低,T1.3時海馬IL-1β含量降低,T1時海馬TNF-α含量升高,T2時海馬IL-10含量降低(P＜0.05),海馬CA1區平均錐體細胞密度差異無統計學意義(P＞0.05),海馬CA1區錐體細胞損傷嚴重;與S-I/R組比較,S-Gur組學習和記憶成績升高,海馬CA1區平均錐體細胞密度增多,T2,3時海馬IL-1β含量降低,T1-4時海馬TNF-α含量降低,T2時海馬IL-10含量升高(P＜0.05),海馬CA1區錐體細胞損傷減輕.結論 薑黃素減輕高血壓病大鼠全腦缺血再灌註損傷的機製可能與抑製海馬炎性反應有關.
목적 평개강황소대고혈압병대서전뇌결혈재관주손상시해마염성반응적영향.방법 선택SPF급웅성Wistar-Kyoto대서48지,8주령,체중180 ～ 200 g,채용수궤수자표법,장기분위2조(n=24):가수술조(W-Sham조)、결혈재관주조(W-1/R조).SPF급웅성자발성고혈압(SH)대서72지,8주령,체중180 ～ 200 g,채용수궤수자표법,장기분위3조(n=24):가수술조(S-Sham조)、결혈재관주조(S-I/R조)、강황소조(S-Cur조).채용사혈관조단법건립전뇌결혈재관주손상모형(결혈10min후회복관주).S-Cur조우재관주30 min시복강주사강황소100 mg/kg,W-Sham조、S-Sham조、W-I/R조화S-I/R조우재관주30 min시복강주사옥미유5ml/kg.우재관주7d시행도태실험측정학습화기억성적,우재관주3h、1、3화7 d(T1-4)시처사취해마조직,소목소-이홍염색후관찰해마CA1구추체세포형태,Nissl염색후계수해마CA1구평균추체세포밀도,ELISA법측정해마IL-1β、TNF-α화IL-10적함량.결과 여W-Sham조비교,W-I/R조학습화기억성적강저,해마CA1구평균추체세포밀도감소,T1-3시해마IL-1β함량승고,T1시해마TNF-α함량승고,해마IL-10함량우T2시승고,T1,3,4시강저(P＜0.05),S-Sham조상술각지표비교차이무통계학의의(P＞0.05);여W-I/R조비교,S-I/R조학습화기억성적강저,T1.3시해마IL-1β함량강저,T1시해마TNF-α함량승고,T2시해마IL-10함량강저(P＜0.05),해마CA1구평균추체세포밀도차이무통계학의의(P＞0.05),해마CA1구추체세포손상엄중;여S-I/R조비교,S-Gur조학습화기억성적승고,해마CA1구평균추체세포밀도증다,T2,3시해마IL-1β함량강저,T1-4시해마TNF-α함량강저,T2시해마IL-10함량승고(P＜0.05),해마CA1구추체세포손상감경.결론 강황소감경고혈압병대서전뇌결혈재관주손상적궤제가능여억제해마염성반응유관.
Objective To evaluate the effect of curcumin on the inflammatory responses in the hippocampus during global cerebral ischemia-reperfusion (I/R) injury in hypertensive rats.Methods Forty-eight SPF male Wistar-Kyoto rats,aged 8 weeks,weighing 180-200 g,were randomly divided into 2 groups (n =24 each) using a random number table:sham operation group (W-Sham group) and I/R group (W-I/R group).Seventy-two SPF male spontaneously hypertensive rats,aged 8 weeks,weighing 180-200 g,were randomly divided into 3 groups (n =24 each) using a random number table:sham operation group (S-Sham group),I/R group (S-I/R group),and curcumin group (S-Cur group).Global cerebral ischemia was induced by 4-vessel occlusion method (10 min of transient global ischemia followed by reperfusion).Curcumin 100 mg/kg was injected intraperitoneally at 30 min of reperfusion in S-Cur group,and corn oil 5 ml/kg was injected intraperitoneally at 30 min of reperfusion in the other groups.The ability of learning and memory was tested by step-down test at 7 days of reperfusion.Rats were sacrificed at 3 h and 1,3 and 7 days of reperfusion (T1-4) and the hippocampi were removed.The morphological changes of pyramidal cells in hippocampal CA1 area were observed by HE staining.The mean density of pyramidal cells in hippocampal CA1 area was quantified by Nissl staining.The contents of interleukin-lβ (IL-1β),tumor necrosis factor-alpha (TNF-α) and IL-10 in the hippocampus were determined by ELISA.Results Compared with W-Sham group,the ability of learning and memory was significantly decreased,the mean density of pyramidal cells in hippocampal CA1 area was decreased,the contents of IL-1β at T1-3,TNF-α at T1,and IL-10 at T2 were increased,and the contents of IL-10 were decreased at T1,3,4 in W-I/R group,and no significant changes were found in the parameters mentioned above in S-Sham group.Compared with W-I/R group,the ability of learning and memory was significantly decreased,the contents of IL-1β at T1,3 and IL-10 at T2 were decreased,the content of TNF-α was increased at T1,no significant change was found in the mean density of pyramidal cells in hippocampal CA1 area,and the damage to pyramidal cells in hippocampal CA1 area was severe in S-I/R group.Compared with S-I/R group,the ability of learning and memory was significantly increased,the mean density of pyramidal cells in hippocampal CA1 area was increased,the contents of IL-1β at T2,3 and TNF-α at T1-4 were increased,and the content of IL-10 was increased at T2,and the damage to pyramidal cells in hippocampal CA1 area was reduced in S-Cur group.Conclusion Inhibition of inflammatory responses in the hippocampus may be involved in the mechanism by which curcumin reduces global cerebral I/R injury in hypertensive rats.
