CAJ | 학술논문

目的评价右美托咪定对神经病理性痛大鼠脊髓背根神经节c-Jun氨基末端激酶(JNK)活化的影响.方法健康成年雄性SD大鼠54只,体重180 ～ 220 g,采用随机数字表法,将其分为3组(n=18):假手术组(S组)、神经病理性痛组(NP组)和右美托咪定组(D组).采用坐骨神经慢性压迫性损伤法建立大鼠神经病理性痛模型.D组于术后即刻腹腔注射右美托咪定50 μg/kg,1次/d,连续14 d.S组和NP组以等容量生理盐水替代.于术前ld、术后3、7和14 d时测定机械缩足反应阈(MWT)和热缩足反应潜伏期(TWL);于术后3、7和14 d测定痛阈后处死,取损伤侧L4-6脊髓背根神经节,采用免疫组化法测定JNK磷酸化(p-JNK)水平.结果与S组比较,NP组和D组术后各时点MWT降低,TWL缩短,脊髓背根神经节p-JNK表达上调(P＜0.05);与NP组比较,D组术后各时点MWT升高,TWL延长,脊髓背根神经节p-JNK表达下调(P＜0.05).结论右美托咪定减轻大鼠神经病理性痛的机制与抑制脊髓背根神经节JNK活化有关.
목적 평개우미탁미정대신경병이성통대서척수배근신경절c-Jun안기말단격매(JNK)활화적영향.방법 건강성년웅성SD대서54지,체중180 ～ 220 g,채용수궤수자표법,장기분위3조(n=18):가수술조(S조)、신경병이성통조(NP조)화우미탁미정조(D조).채용좌골신경만성압박성손상법건립대서신경병이성통모형.D조우술후즉각복강주사우미탁미정50 μg/kg,1차/d,련속14 d.S조화NP조이등용량생리염수체대.우술전ld、술후3、7화14 d시측정궤계축족반응역(MWT)화열축족반응잠복기(TWL);우술후3、7화14 d측정통역후처사,취손상측L4-6척수배근신경절,채용면역조화법측정JNK린산화(p-JNK)수평.결과 여S조비교,NP조화D조술후각시점MWT강저,TWL축단,척수배근신경절p-JNK표체상조(P＜0.05);여NP조비교,D조술후각시점MWT승고,TWL연장,척수배근신경절p-JNK표체하조(P＜0.05).결론 우미탁미정감경대서신경병이성통적궤제여억제척수배근신경절JNK활화유관.
Objective To evaluate the effects of dexmedetomidine on activation of c-Jun N-terminal kinase (JNK) in the dorsal root ganglions of rats with neuropathic pain (NP).Methods Fifty-four male Wistar rats,weighing 180-220 g,were randomly divided into 3 groups with 18 animals in each group using a random number table:sham operation group (group S),NP group and dexmedetomidine group (group D).The animals were anesthetized with intraperitoneal chloral hydrate.NP was induced by chronic constrictive injury.In group S,the right sciatic nerves were only exposed,but not ligated.In group D,dexmedetomidine 50 μg/kg was intraperitoneally injected immediately after operation once a day for 14 consecutive days.In NP and S groups,the rats received the equal volume of normal saline instead of dexmedetomidine.At 1 day before operation (T0) and 3,7 and 14 days after operation (T1-3),mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured.The rats in each group were sacrificed after measurement of pain threshold at T1-3 and the ipsilateral dorsal root ganglions of the lumbar segment (L4-6) were removed to detect the phosphorylation of JNK (p-JNK) using immunohistochemistry.Results Compared with group S,MWT was significantly decreased,TWL was shortened,and the expression of p-JNK was up-regulated at each time point after operation in NP and D groups.Compared with group NP,MWT was significantly increased,TWL was prolonged,and the expression of p-JNK was down-regulated at each time point after operation in group D.Conclusion The mechanism by which dexmedetomidine attenuates NP is related to inhibition of activation of JNK in the dorsal root ganglions of rats.