中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2009年
5期
554-557
,共4页
南静%邓华聪%陈丹燕%冯正平%周恒宇%秦登优%刘强
南靜%鄧華聰%陳丹燕%馮正平%週恆宇%秦登優%劉彊
남정%산화총%진단연%풍정평%주항우%진등우%류강
INS-1细胞%葡萄糖%球型脂联素%AMP活化的蛋白激酶
INS-1細胞%葡萄糖%毬型脂聯素%AMP活化的蛋白激酶
INS-1세포%포도당%구형지련소%AMP활화적단백격매
INS-1 cells%Glucose%Globular adiponectin%AMP-activated protein kinase
以3、11、20 mmol/L葡萄糖或在5 mmol/L葡萄糖浓度时以2.5 mg/L球形脂联素孵育INS-1细胞24 h,RT-PCR检测PPARa、肉毒碱棕榈酸转移酶1(CPT1)和酰基辅酶A氧化酶(ACO)mRNA的表达.结果 显示,随着葡萄糖浓度的升高,INS-1细胞PPARa和CPT1、ACO mRNA的表达降低(均P＜0.05),球形脂联素增加PPARα(0.945±0.153 vs 0.749±0.069)、CPT1(0.590±0.029 vs 0.388±0.037)和ACO(1.553±0.131 vs 1.287±0.224)mRNA的表达(均P＜0.05),可被AMP活化的蛋白激酶(AMPK)抑制剂阿糖腺苷所抑制(分别为0.360±0.113、0.248±0.037和0.942±0.303,均P＜0.05).
以3、11、20 mmol/L葡萄糖或在5 mmol/L葡萄糖濃度時以2.5 mg/L毬形脂聯素孵育INS-1細胞24 h,RT-PCR檢測PPARa、肉毒堿棕櫚痠轉移酶1(CPT1)和酰基輔酶A氧化酶(ACO)mRNA的錶達.結果 顯示,隨著葡萄糖濃度的升高,INS-1細胞PPARa和CPT1、ACO mRNA的錶達降低(均P＜0.05),毬形脂聯素增加PPARα(0.945±0.153 vs 0.749±0.069)、CPT1(0.590±0.029 vs 0.388±0.037)和ACO(1.553±0.131 vs 1.287±0.224)mRNA的錶達(均P＜0.05),可被AMP活化的蛋白激酶(AMPK)抑製劑阿糖腺苷所抑製(分彆為0.360±0.113、0.248±0.037和0.942±0.303,均P＜0.05).
이3、11、20 mmol/L포도당혹재5 mmol/L포도당농도시이2.5 mg/L구형지련소부육INS-1세포24 h,RT-PCR검측PPARa、육독감종려산전이매1(CPT1)화선기보매A양화매(ACO)mRNA적표체.결과 현시,수착포도당농도적승고,INS-1세포PPARa화CPT1、ACO mRNA적표체강저(균P＜0.05),구형지련소증가PPARα(0.945±0.153 vs 0.749±0.069)、CPT1(0.590±0.029 vs 0.388±0.037)화ACO(1.553±0.131 vs 1.287±0.224)mRNA적표체(균P＜0.05),가피AMP활화적단백격매(AMPK)억제제아당선감소억제(분별위0.360±0.113、0.248±0.037화0.942±0.303,균P＜0.05).
INS-1 cells were treated with 3,11,20 mmol/L glucose or 2.5 mg/L globular adiponectin at the concentration of 5.5 mmol/L glucose for 24 h.The levels of PPARα,carnitine palmitoyhransferase Ⅰ (CPT1) and acyl-CoA oxidase (ACO) mRNA were detected by RT-PCR.The results showed that PPARα,PPT1 and ACO mRNA expressions were down-regulated with the increase of glucose concentration(all P＜0.05).Globular adiponectin increased PPARα (0.945±0.153 vs 0.749 ± 0.069),CPT1 (0.590±0.029 vs 0.388± 0.037) and ACO (1.553±0.131 vs 1.287±0.224)mRNA expressions(all P＜0.05),which were inhibited by AMP-activated protein kinase inhibitor adenine 9-β-D-arabinfuranoside (0.360±0.113,0.248±0.037 and 0.942±0.303,respectively,all P＜0.05).
