CAJ | 학술논문

目的体外观察罗格列酮对骨髓间充质干细胞(BMSCs)向成骨细胞分化的影响,并观察对碱性磷酸酶(ALP)、骨形成蛋白-2(BMP-2)、转化生长因子β1(TGF-β1)分泌的影响,探讨其对骨代谢的作用机制.方法无菌条件下从大鼠长骨骨髓中分离获取BMSCs,采用全骨髓贴壁培养法对BMSCs进行纯化、传代扩增,随后在1、2、5、10 μmol/L罗格列酮干预下诱导成骨细胞分化培养21 d,进行茜素红染色观察矿化结节,并测定成骨细胞标记物ALP、BMP-2及TGF-β1的分泌.结果 1、2、5、10 μmol/L罗格列酮干预组与成骨经典组对比,成骨细胞的钙结节形成比例显著降低(P＜0.05),ALP、BMP-2、TGF-β1水平呈剂量依赖性地下降(均P＜0.05).结论罗格列酮可剂量依赖性地抑制BMSCs向成骨细胞分化,这可能是罗格列酮致骨质疏松的重要机制.
목적 체외관찰라격렬동대골수간충질간세포(BMSCs)향성골세포분화적영향,병관찰대감성린산매(ALP)、골형성단백-2(BMP-2)、전화생장인자β1(TGF-β1)분비적영향,탐토기대골대사적작용궤제.방법 무균조건하종대서장골골수중분리획취BMSCs,채용전골수첩벽배양법대BMSCs진행순화、전대확증,수후재1、2、5、10 μmol/L라격렬동간예하유도성골세포분화배양21 d,진행천소홍염색관찰광화결절,병측정성골세포표기물ALP、BMP-2급TGF-β1적분비.결과 1、2、5、10 μmol/L라격렬동간예조여성골경전조대비,성골세포적개결절형성비례현저강저(P＜0.05),ALP、BMP-2、TGF-β1수평정제량의뢰성지하강(균P＜0.05).결론 라격렬동가제량의뢰성지억제BMSCs향성골세포분화,저가능시라격렬동치골질소송적중요궤제.
Objective To observe the effects of rosiglitazone on differentiation of rat bone-marrow stromal cells (BMSCs) into osteoblasts (OB) and on secretion of alkaline phosphatase (ALP), bone morphogenetic protein2 (BMP-2), and transforming growth factor-β1 (TGF-β1) in order to investigate its mechanism of the impact on the bone metabolism.Methods BMSCs from long bone were bred by using differential time adherent culture method,and then were interfered with 1,2,5,10 μmol/L rosiglitazone to differentiate into osteoblasts in the presence of an osteogenic medium.The rate of mineralization was examined by staining mineralized nodules with Alizarin red S,and the secretion of ALP, BMP-2, and TGF-β1 was examined by enzyme linked immunosorbent assay (ELISA)after 21 d of culture.Results Compared with the classic group, the rate of mineralization was significantly decreased by 1,2,5 and 10 μmoL/L rosiglitazone (P＜0.05), the levels of ALP, BMP-2, and TGF-β1 decreased in a dose-depedent manner (P＜0.05).Conclusion Rosiglitazone dose-dependently inhibits differentiation of BMSCs into osteoblasts, which may be an important mechanism in causing osteoporosis.