中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2011年
1期
62-65
,共4页
李刚%丛丽%殷娜%李强%王薇%张巾超
李剛%叢麗%慇娜%李彊%王薇%張巾超
리강%총려%은나%리강%왕미%장건초
脂联素%胰岛素%β肾上腺素受体激动剂%胰岛素钳夹试验
脂聯素%胰島素%β腎上腺素受體激動劑%胰島素鉗夾試驗
지련소%이도소%β신상선소수체격동제%이도소겸협시험
Adiponectin%Insulin%β-adrenergic agonists%Insulin clamp
目的 在体探讨大鼠脂联素表达和分泌的调节机制.方法 大鼠在空腹后重新进食、高胰岛素钳夹试验、应用β肾上腺素受体激动剂情况下,放射免疫法测定血浆中脂联素水平,实时定量PCR方法检测脂肪组织脂联素基因表达的变化.结果 (1)大鼠空腹18 h后,进食可显著增加血浆脂联素水平(约2倍)和脂肪组织脂联素的表达(约3倍),且此作用可被β肾上腺素受体激动剂所拮抗.(2)大鼠空腹18 h后,高胰岛素钳夹可显著增加血浆脂联素水平和脂肪组织脂联素的表达.结论 在体内,脂联素的合成和分泌可迅速被营养状态所调节.胰岛素和β肾上腺素受体激动剂可调节脂肪组织脂联素的分泌和表达.
目的 在體探討大鼠脂聯素錶達和分泌的調節機製.方法 大鼠在空腹後重新進食、高胰島素鉗夾試驗、應用β腎上腺素受體激動劑情況下,放射免疫法測定血漿中脂聯素水平,實時定量PCR方法檢測脂肪組織脂聯素基因錶達的變化.結果 (1)大鼠空腹18 h後,進食可顯著增加血漿脂聯素水平(約2倍)和脂肪組織脂聯素的錶達(約3倍),且此作用可被β腎上腺素受體激動劑所拮抗.(2)大鼠空腹18 h後,高胰島素鉗夾可顯著增加血漿脂聯素水平和脂肪組織脂聯素的錶達.結論 在體內,脂聯素的閤成和分泌可迅速被營養狀態所調節.胰島素和β腎上腺素受體激動劑可調節脂肪組織脂聯素的分泌和錶達.
목적 재체탐토대서지련소표체화분비적조절궤제.방법 대서재공복후중신진식、고이도소겸협시험、응용β신상선소수체격동제정황하,방사면역법측정혈장중지련소수평,실시정량PCR방법검측지방조직지련소기인표체적변화.결과 (1)대서공복18 h후,진식가현저증가혈장지련소수평(약2배)화지방조직지련소적표체(약3배),차차작용가피β신상선소수체격동제소길항.(2)대서공복18 h후,고이도소겸협가현저증가혈장지련소수평화지방조직지련소적표체.결론 재체내,지련소적합성화분비가신속피영양상태소조절.이도소화β신상선소수체격동제가조절지방조직지련소적분비화표체.
Objective To explore the potential mechanisms of regulating adiponectin secretion and expression in vivo in rats.Methods To observe the regulation of adiponectin by fasing-refeeding and β-adrenergic agonists, male Wistar rats were fasted for 18 h and allowed to refeed or a β3-adrenergic receptor agonist was infused into refeeding rats.The effects of insulin clamp on adiponectin secretion and expression, including euglycemichyperinsulinemic clamp and hyperglycemic-hyperinsulinemic clamp, were also investigated.Plasma adiponectin level was determined by radioimmunoassay.Adiponectin mRNA expression in adipose tissue of rats was detected by realtime PCR.Results (1) Refeeding 18 h fasted rats increased plasma adiponectin concentration (about 2-fold) and adipose tissue adiponectin expression (about 3-fold), which were completely blocked by administration of β-adrenergic agonist.(2) Hyperinsulinemic clamp increased plasma adiponectin concentration and adiponectin gene expression in adipose tissue.Conclusions Adiponectin secretion and expression are acutely regulated in vivo by nutritional status.Insulin and β-adrenergic agonists regulate adiponectin secretion and expression in adipose tissue.
