CAJ | 학술논문

二甲双胍干预高糖环境下成骨细胞MG63,应用CCK-8检测细胞增殖,SFBC速率法检测细胞内碱性磷酸酶活性,RT-PCR法检测细胞相关基因的表达.干预后二甲双胍可促进高糖环境中成骨细胞MG63增殖(P＜0.01)；增加碱性磷酸酶活性(P＜0.05)；上调Ⅰ型胶原和骨钙素的表达,同时抑制基质金属蛋白酶(MMP-1、MMP-2、MMP-3和MMP-8)的表达(P＜0.05).结果提示二甲双胍可能通过促进成骨细胞增殖、分化和矿化,抑制成骨细胞外胶原基质的降解,发挥对成骨细胞的保护作用.
이갑쌍고간예고당배경하성골세포MG63,응용CCK-8검측세포증식,SFBC속솔법검측세포내감성린산매활성,RT-PCR법검측세포상관기인적표체.간예후이갑쌍고가촉진고당배경중성골세포MG63증식(P＜0.01)；증가감성린산매활성(P＜0.05)；상조Ⅰ형효원화골개소적표체,동시억제기질금속단백매(MMP-1、MMP-2、MMP-3화MMP-8)적표체(P＜0.05).결과제시이갑쌍고가능통과촉진성골세포증식、분화화광화,억제성골세포외효원기질적강해,발휘대성골세포적보호작용.
The proliferation of MG63 cells under high glucose intervened with metformin was measured by CCK-8 assay.The activity of intracellular alkaline phosphatase (ALP) was detected by SFBC assay.The expression of related genes was detected by RT-PCR.Metformin promoted the proliferation of MG63 cells (P＜0.01),the activity of ALP was increased (P＜0.05).The expressions of collagen Ⅰ and osteocalcin were up-regulated while the expressions of matrix metalloproteinase (MMP)-1,MMP-2,MMP-3,and MMP-8 were down-regulated (P＜0.05).The results indicate that metformin may protect osteoblast,via promoting osteoblast proliferation,differentiation and mineralization,as well as by inhibiting degradation of osteoblast extracellular collagen matrix.