CAJ | 학술논문

目的明确适配蛋白APPL1在胰岛β细胞中的作用.方法采用腺病毒转染使INS-1细胞过表达APPL1.Western印迹法检测蛋白表达水平,碘化丙啶/Hoechst染色法检测细胞凋亡,用ELISA检测胰岛素分泌.结果 20 mmol/L葡萄糖或30 U/ml白细胞介素-1β加20 ng/ml TNF-α作用INS-1细胞48h后,可明显诱导胰岛β细胞凋亡(P＜0.01),并降低2h葡萄糖刺激的胰岛素分泌水平(P＜0.01).INS-1细胞过表达APPL1后,与各自的对照病毒组相比,APPL1可使细胞凋亡降低34.16％～42.79％ (P＜0.01),并使葡萄糖刺激的胰岛素分泌升高至对照组的1.39 ～2.20倍(P＜0.05).结论 APPL1对高糖和炎症因子诱导的胰岛β细胞损伤具有保护作用,可降低β细胞凋亡并改善葡萄糖刺激的胰岛素分泌.
목적 명학괄배단백APPL1재이도β세포중적작용.방법 채용선병독전염사INS-1세포과표체APPL1.Western인적법검측단백표체수평,전화병정/Hoechst염색법검측세포조망,용ELISA검측이도소분비.결과 20 mmol/L포도당혹30 U/ml백세포개소-1β가20 ng/ml TNF-α작용INS-1세포48h후,가명현유도이도β세포조망(P＜0.01),병강저2h포도당자격적이도소분비수평(P＜0.01).INS-1세포과표체APPL1후,여각자적대조병독조상비,APPL1가사세포조망강저34.16％～42.79％ (P＜0.01),병사포도당자격적이도소분비승고지대조조적1.39 ～2.20배(P＜0.05).결론 APPL1대고당화염증인자유도적이도β세포손상구유보호작용,가강저β세포조망병개선포도당자격적이도소분비.
Objective To determine the role of APPL1,an adaptor protein,played in pancreatic β-cell.Methods APPL1 was overexpressed in INS-1 cells with adenovirus encoding APPL1.Western blot was conducted to measure protein cxprcssion.Propidium iodide/Hoechst staining was used to determine the cell apoptosis.Insulin secretion was measured by ELISA.Results Exposure of INS-1 cells to 20 mmol/L glucose or 30U/ml interleukin-1 β plus 20 ng/ml TNF-α 48 h induced β-cell apoptosis (P＜0.01) and impaired 2 h glucosestimulated insulin secretion (P＜ 0.01).Overexpression of APPL1 in INS-1 decreased cell apoptosis by 34.16％-42.79％ (P＜0.01) and increased glucose-induced insulin secretion by 1.39-2.20 folds compared with control groups (P＜0.05).Conclusion APPL1 decreases β-cell apoptosis and increases glucose-stimulated insulin secretion,and thus protects β-cell against high glucose or cytokines-induced dysfunction.