中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2013年
5期
375-378
,共4页
戴娟%张光波%高楠%奚沁华%李月芹%庞智%赵毅%赵坚敏%聂锦山
戴娟%張光波%高楠%奚沁華%李月芹%龐智%趙毅%趙堅敏%聶錦山
대연%장광파%고남%해심화%리월근%방지%조의%조견민%섭금산
炎性肠疾病%T淋巴细胞%Th1细胞%Th17细胞
炎性腸疾病%T淋巴細胞%Th1細胞%Th17細胞
염성장질병%T림파세포%Th1세포%Th17세포
Inflammatory bowel diseases%T-lymphocytes%Th1 cells%Th17 cells
目的 探讨炎症性肠病(IBD)患者外周血Th1和Th17细胞水平对IBD发病及活动度的意义.方法 收集2011年5月至2012年7月健康对照者40例和IBD患者81例[其中克罗恩病(CD)39例,溃疡性结肠炎(UC)42例],采集外周血标本.分离外周血单个核细胞,经PMA及伊屋诺霉素联合刺激、培养后,利用流式细胞仪检测Th1和Th17细胞在外周血CD4+T细胞中的百分比,并结合临床资料分析其临床意义.结果 (1)CD组及UC组Th1细胞百分比[(38.32±16.18)％和(34.23±11.60)％]均明显高于健康对照组[(24.58±10.02)％](P值均＜0.01),而CD组及UC组的差异无统计学意义；CD及UC缓解期患者的Th1细胞百分比均低于活动期患者[(26.50±9.24)％比(48.46±13.83)％,P＜0.01;(30.05±7.41)％比(37.68±13.35)％,P＜0.05].(2)CD组及UC组Th17细胞百分比[(2.51±1.59)％和(4.15±2.75)％]均高于健康对照组[(1.44±0.73)％](P值均＜ 0.01),且UC组这一比例高于CD组(P＜0.01)；CD及UC活动期患者的Th17细胞百分比明显高于缓解期患者[(3.39±1.56)％比(1.48±0.81)％,(5.77±2.77)％比(2.18±0.59)％,P值均＜0.01].(3)UC组外周血 Th17/Th1比值(0.14±0.11)高于CD组(0.08±0.06)和健康对照组(0.07±0.06),P值均＜0.01.结论 IBD患者外周血中Th1、Th17细胞占CD4+ T细胞比例较健康人群明显升高,且与IBD活动度密切相关.Th1和Th17细胞在IBD发病中可能具有重要作用.
目的 探討炎癥性腸病(IBD)患者外週血Th1和Th17細胞水平對IBD髮病及活動度的意義.方法 收集2011年5月至2012年7月健康對照者40例和IBD患者81例[其中剋囉恩病(CD)39例,潰瘍性結腸炎(UC)42例],採集外週血標本.分離外週血單箇覈細胞,經PMA及伊屋諾黴素聯閤刺激、培養後,利用流式細胞儀檢測Th1和Th17細胞在外週血CD4+T細胞中的百分比,併結閤臨床資料分析其臨床意義.結果 (1)CD組及UC組Th1細胞百分比[(38.32±16.18)％和(34.23±11.60)％]均明顯高于健康對照組[(24.58±10.02)％](P值均＜0.01),而CD組及UC組的差異無統計學意義；CD及UC緩解期患者的Th1細胞百分比均低于活動期患者[(26.50±9.24)％比(48.46±13.83)％,P＜0.01;(30.05±7.41)％比(37.68±13.35)％,P＜0.05].(2)CD組及UC組Th17細胞百分比[(2.51±1.59)％和(4.15±2.75)％]均高于健康對照組[(1.44±0.73)％](P值均＜ 0.01),且UC組這一比例高于CD組(P＜0.01)；CD及UC活動期患者的Th17細胞百分比明顯高于緩解期患者[(3.39±1.56)％比(1.48±0.81)％,(5.77±2.77)％比(2.18±0.59)％,P值均＜0.01].(3)UC組外週血 Th17/Th1比值(0.14±0.11)高于CD組(0.08±0.06)和健康對照組(0.07±0.06),P值均＜0.01.結論 IBD患者外週血中Th1、Th17細胞佔CD4+ T細胞比例較健康人群明顯升高,且與IBD活動度密切相關.Th1和Th17細胞在IBD髮病中可能具有重要作用.
목적 탐토염증성장병(IBD)환자외주혈Th1화Th17세포수평대IBD발병급활동도적의의.방법 수집2011년5월지2012년7월건강대조자40례화IBD환자81례[기중극라은병(CD)39례,궤양성결장염(UC)42례],채집외주혈표본.분리외주혈단개핵세포,경PMA급이옥낙매소연합자격、배양후,이용류식세포의검측Th1화Th17세포재외주혈CD4+T세포중적백분비,병결합림상자료분석기림상의의.결과 (1)CD조급UC조Th1세포백분비[(38.32±16.18)％화(34.23±11.60)％]균명현고우건강대조조[(24.58±10.02)％](P치균＜0.01),이CD조급UC조적차이무통계학의의；CD급UC완해기환자적Th1세포백분비균저우활동기환자[(26.50±9.24)％비(48.46±13.83)％,P＜0.01;(30.05±7.41)％비(37.68±13.35)％,P＜0.05].(2)CD조급UC조Th17세포백분비[(2.51±1.59)％화(4.15±2.75)％]균고우건강대조조[(1.44±0.73)％](P치균＜ 0.01),차UC조저일비례고우CD조(P＜0.01)；CD급UC활동기환자적Th17세포백분비명현고우완해기환자[(3.39±1.56)％비(1.48±0.81)％,(5.77±2.77)％비(2.18±0.59)％,P치균＜0.01].(3)UC조외주혈 Th17/Th1비치(0.14±0.11)고우CD조(0.08±0.06)화건강대조조(0.07±0.06),P치균＜0.01.결론 IBD환자외주혈중Th1、Th17세포점CD4+ T세포비례교건강인군명현승고,차여IBD활동도밀절상관.Th1화Th17세포재IBD발병중가능구유중요작용.
Objective To explore the probable role of Th1 and Th17 cells in the pathogenesis of inflammatory bowel disease (IBD).Methods The peripheral blood mononuclear cells (PBMCs) from peripheral blood specimens were collected in the study,including 40 healthy controls,42 ulcerative colitis (UC) and 39 Crohn's disease (CD).The proportion of Th1 and Th17 cells in the PBMCs was detected with flow cytometry after stimulated by PMA and ionomycin.The result and the clinical data were analyzed.Results The Th1 cell expression was increased in CD (38.32 ± 16.18)％ and UC group (34.23 ±11.60) ％,compared with the controls (24.58 ± 10.02) ％ (P ＜ 0.01).During the convalescence,the Th1expression in the CD and UC groups in vivo was significantly reduced without difference between the two groups(P ＞0.05).In the IBD group,significant difference in the frequency of Th17 cells could be found between the CD group(2.51 ±1.59)％ and the UC group(4.15 ±2.75)％,while the Th17 cells were increased in both groups,compared with the controls (1.44 ± 0.73) ％ (P ＜ 0.05).Obvious difference in the frequency of Th17 cells could be found between patients at different activity stages and remission stages.The proportion of Th17 cells were higher in the UC patients than that in the CD patients (P ＜ 0.01).The Th1 7/Th1 ratio of CD patients,UC patients were 0.08 ± 0.06,0.14 ± 0.11,which were both higher that in the controls(0.07 ± 0.06).Significant difference could be found between the UC group and the CD group (P ＜ 0.01).Conclusions The higher proportion of Th1 and Th17 cells are detected in the peripheral blood of IBD patients,which is correlated closely to the activity of the disease.Th1 and Th17 cells may play an important role in the pathogenesis of IBD.
