CAJ | 학술논문

目的通过检测系统性红斑狼疮(SLE)患者外周血白细胞双链RNA依赖蛋白激酶(PKR)基因表达水平,探讨其与SLE临床及病情活动的相关性.方法分离100例SLE患者、40例非SLE患者、40例健康人外周血白细胞,抽提总RNA并逆转录成cDNA,实时定量PCR检测所有受试者PKR基因表达水平,并进行相关性分析.以2-△Ct代表PKR mRNA的起始拷贝量.结果 (1)SLE患者PKR mRNA起始拷贝量(14.69±7.62)高于非SLE患者(5.09±4.73;P =0.012)和健康人(4.79±3.49;P =0.005),差异有统计学意义.(2) SLE疾病活动指数(SLEDAI)≥15分的SLE患者PKR mRNA起始拷贝量为22.57±2.61,明显高于SLEDAI≤4分(8.85±2.17;P=0.000)和SLEDAI5～14分的SLE患者(12.94±2.41；P=0.000).(3)有狼疮肾炎的SLE患者PKR mRNA起始拷贝量(16.85 ±7.32)高于无狼疮肾炎的SLE患者(8.35±2.04;P =0.034).(4)SLE患者PKR mRNA起始拷贝量与SLEDAI、外周血白细胞计数、24 h尿蛋白、血清HDL-C呈正相关(r=0.32,P=0.000;r=0.46,P=0.000;r =0.21,P=0.000；r=0.21,P=0.022),与Hb、抗核糖核蛋白(RNP)抗体水平呈负相关(r=-0.22,P=0.035；rs=-0.21,P=0.025).结论 SLE患者外周血白细胞PKR基因表达上调,且在重度SLE患者中更明显,提示PKR基因可能与狼疮的发病相关.
목적 통과검측계통성홍반랑창(SLE)환자외주혈백세포쌍련RNA의뢰단백격매(PKR)기인표체수평,탐토기여SLE림상급병정활동적상관성.방법 분리100례SLE환자、40례비SLE환자、40례건강인외주혈백세포,추제총RNA병역전록성cDNA,실시정량PCR검측소유수시자PKR기인표체수평,병진행상관성분석.이2-△Ct대표PKR mRNA적기시고패량.결과 (1)SLE환자PKR mRNA기시고패량(14.69±7.62)고우비SLE환자(5.09±4.73;P =0.012)화건강인(4.79±3.49;P =0.005),차이유통계학의의.(2) SLE질병활동지수(SLEDAI)≥15분적SLE환자PKR mRNA기시고패량위22.57±2.61,명현고우SLEDAI≤4분(8.85±2.17;P=0.000)화SLEDAI5～14분적SLE환자(12.94±2.41；P=0.000).(3)유랑창신염적SLE환자PKR mRNA기시고패량(16.85 ±7.32)고우무랑창신염적SLE환자(8.35±2.04;P =0.034).(4)SLE환자PKR mRNA기시고패량여SLEDAI、외주혈백세포계수、24 h뇨단백、혈청HDL-C정정상관(r=0.32,P=0.000;r=0.46,P=0.000;r =0.21,P=0.000；r=0.21,P=0.022),여Hb、항핵당핵단백(RNP)항체수평정부상관(r=-0.22,P=0.035；rs=-0.21,P=0.025).결론 SLE환자외주혈백세포PKR기인표체상조,차재중도SLE환자중경명현,제시PKR기인가능여랑창적발병상관.
Objective To investigate the expression of the double-stranded RNA-dependent protein kinase (PKR) gene in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE),and to evaluate the relationship between the gene expression and the disease activity.Methods The clinical data of 100 SLE patients,40 non-SLE patients with rheumatic diseases,and 40 normal controls were collected.Total RNA was extracted from the peripheral blood and then reverse transcribed into cDNA.Sybr green dye based real-time quantitative PCR method was used to compare the expression levels (indicated as 2-△Ct value) of PKR in the three groups.Results (1) The 2-△Ct value of PKR expression level in the SLE patients was (14.69 ± 7.62),which was significantly higher than those in the non-SLE patients (5.09 ±4.73,P =0.012) and normal controls (4.79 ± 3.49,P =0.005).(2) The 2-△Ct value of PKR expression level in the SLE patients with severe activity was (22.57 ±2.61),which was significantly higher than those in the SLE patients with mild activity and no activity(12.94±2.41,P =0.000 ;8.85 ± 2.17,P =0.000).(3) The 2 △Ct value of PKR expression level in the SLE patients with lupus nephritis was significantly higher than that in the SLE patients without lupus nephritis (16.85 ± 7.32 vs 8.35 ± 2.04,P =0.034).(4) The 2-△Ct value of PKR was correlated with the systemic lupus erythematosus index(SLEDAI) scores(r =0.32,P =0.000),WBC (r =0.46,P =0.000),Hb (r =-0.22,P =0.035),the quantitation of urine protein in 24 hours (r =0.21,P =0.000),HDL-C (r =0.21,P =0.022),and anti-RNP antibody (rs =-0.21,P =0.025).Conclusions The expression of PKR in the SLE patients is up-regulated,especially in those with severe activity.The expression level of PKR gene is associated with SLE disease activity.