中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2014年
10期
799-803
,共5页
俞俐琴%夏盛隆%郑波%郭茂东%林心心%林秀清%姜利佳%丁然%蒋益
俞俐琴%夏盛隆%鄭波%郭茂東%林心心%林秀清%薑利佳%丁然%蔣益
유리금%하성륭%정파%곽무동%림심심%림수청%강리가%정연%장익
结肠炎,溃疡性%血管内皮生长因子类%多态性,单核苷酸
結腸炎,潰瘍性%血管內皮生長因子類%多態性,單覈苷痠
결장염,궤양성%혈관내피생장인자류%다태성,단핵감산
Colitis,ulcerative%Vascular endothelial growth factors%Polymorphism,single nucleotide
目的 探讨血管内皮生长因子(VEGF)基因(-2578C/A)和(+936C/T)位点单核苷酸多态性与溃疡性结肠炎(UC)易感性的关系.方法 收集UC患者373例和健康对照者503例,采用微测序技术检测VEGF基因(-2578C/A)和(+936C/T)单核苷酸多态性.结果 经非条件logistic 回归分析发现,重度UC患者中VEGF(+ 936C/T)的突变等位基因T和基因型CT+TT频率明显低于对照组(10.4％比19.3％,OR=0.487,95％ CI 0.248～0.954,P=0.036;18.8％比33.8％,OR=0.452,95％ CI 0.214 ～0.955,P=0.037).并且重度UC患者中该位点的突变等位基因T和基因型CT+TT频率亦明显低于轻中度患者(10.4％比20.5％,OR=0.452,95％ CI0.229 ～0.894,P=0.022;18.8％比36.9％,OR=0.394,95％ CI 0.185～0.842,P=O.016).但VEGF(-2578C/A)位点的突变等位基因A和基因型CA+ AA频率在UC组与对照组之间差异均无统计学意义,并且与UC患者的临床病理特征亦无关(P均＞0.05).结论 VEGF(+ 936C/T)位点基因突变能影响UC疾病严重程度;而VEGF(-2578C/A)位点基因多态性与UC易感性无关.
目的 探討血管內皮生長因子(VEGF)基因(-2578C/A)和(+936C/T)位點單覈苷痠多態性與潰瘍性結腸炎(UC)易感性的關繫.方法 收集UC患者373例和健康對照者503例,採用微測序技術檢測VEGF基因(-2578C/A)和(+936C/T)單覈苷痠多態性.結果 經非條件logistic 迴歸分析髮現,重度UC患者中VEGF(+ 936C/T)的突變等位基因T和基因型CT+TT頻率明顯低于對照組(10.4％比19.3％,OR=0.487,95％ CI 0.248～0.954,P=0.036;18.8％比33.8％,OR=0.452,95％ CI 0.214 ～0.955,P=0.037).併且重度UC患者中該位點的突變等位基因T和基因型CT+TT頻率亦明顯低于輕中度患者(10.4％比20.5％,OR=0.452,95％ CI0.229 ～0.894,P=0.022;18.8％比36.9％,OR=0.394,95％ CI 0.185～0.842,P=O.016).但VEGF(-2578C/A)位點的突變等位基因A和基因型CA+ AA頻率在UC組與對照組之間差異均無統計學意義,併且與UC患者的臨床病理特徵亦無關(P均＞0.05).結論 VEGF(+ 936C/T)位點基因突變能影響UC疾病嚴重程度;而VEGF(-2578C/A)位點基因多態性與UC易感性無關.
목적 탐토혈관내피생장인자(VEGF)기인(-2578C/A)화(+936C/T)위점단핵감산다태성여궤양성결장염(UC)역감성적관계.방법 수집UC환자373례화건강대조자503례,채용미측서기술검측VEGF기인(-2578C/A)화(+936C/T)단핵감산다태성.결과 경비조건logistic 회귀분석발현,중도UC환자중VEGF(+ 936C/T)적돌변등위기인T화기인형CT+TT빈솔명현저우대조조(10.4％비19.3％,OR=0.487,95％ CI 0.248～0.954,P=0.036;18.8％비33.8％,OR=0.452,95％ CI 0.214 ～0.955,P=0.037).병차중도UC환자중해위점적돌변등위기인T화기인형CT+TT빈솔역명현저우경중도환자(10.4％비20.5％,OR=0.452,95％ CI0.229 ～0.894,P=0.022;18.8％비36.9％,OR=0.394,95％ CI 0.185～0.842,P=O.016).단VEGF(-2578C/A)위점적돌변등위기인A화기인형CA+ AA빈솔재UC조여대조조지간차이균무통계학의의,병차여UC환자적림상병리특정역무관(P균＞0.05).결론 VEGF(+ 936C/T)위점기인돌변능영향UC질병엄중정도;이VEGF(-2578C/A)위점기인다태성여UC역감성무관.
Objective To investigate the association of (-2578C/A) and (+ 936C/T) single nucleotide polymorphism(SNPs) of vascular endothelial growth factor (VEGF) gene with the susceptibility to ulcerative colitis (UC).Methods A total of 373 UC patients and 503 healthy controls were recruited.The (-2578C/A) and (+ 936C/T) polymorphism of VEGF gene were detected using a mini-sequencing technique.Results By an unconditional logistic regression analysis,the frequencies of the mutant allele T and genotype CT +TT of VEGF gene (+936C/T) were significantly decreased in patients with severe UC compared to the controls (10.4％ vs 19.3％,OR =0.487,95％ CI 0.248-0.954,P =0.036 ; 18.8％ vs 33.8％,OR =0.452,95％ CI 0.214-0.955,P =0.037,respectively).Moreover,patients with severe UC had significant lower rates of mutant allele T and genotype CT + TT compared with patients with mild and moderate UC (10.4％ vs 20.5％,OR =0.452,95％ CI 0.229-0.894,P =0.022; 18.8％ vs 36.9％,OR=0.394,95％ CI 0.185-0.842,P =0.016,respectively).The frequencies of mutant allele A and genotype CA + AA of VEGF (-2578C/A) gene were not statistically different between UC patients and the controls.Moreover,they were not significantly associated with the clinicopathologic features in UC patients.Conclusions The mutation of VEGF (+ 936C/T) gene is correlated with the severity of UC.However,the polymorphism of VEGF (-2578C/A) gene is not significantly related to the susceptibility to UC.
