中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
12期
1734-1737
,共4页
瞿彬%张蓉%杨云霜%李延晖
瞿彬%張蓉%楊雲霜%李延暉
구빈%장용%양운상%리연휘
中药%辐射%大鼠%骨髓%造血功能
中藥%輻射%大鼠%骨髓%造血功能
중약%복사%대서%골수%조혈공능
Chinese medicine%Radiation%Rats%Marrow%Hematopoietic function
目的 观察不同方药对辐射造血系统大鼠的干预作用.方法 将150只健康Wistar雄性大鼠完全随机分为空白对照组、辐射模型组、模型+八珍组、模型+六味组和模型+自拟方组,每组30只.除空白对照组外,其余4组均分别施予60Coγ射线全身照射,吸收剂量分别为7 Gy.分别于照射后第1、14、30天取材,每组每次10只,10％水合氯醛腹腔麻醉,腹主动脉采血进行血常规测定,观察外周血网织红细胞微核;取大鼠股骨标本,制作骨髓切片,电镜观察骨髓标本病理形态学的变化,分析辐射对造血系统损伤及中药对辐射损伤干预作用.结果 经放射线照射后,各实验组白细胞均下降,中性粒细胞比值上升.白细胞计数动态变化:照射1d后辐射模型组为(10只)(1.04±0.00)×109/L,模型+八珍组为(10只)(1.80±0.05)×109/L,模型+六味组(10只)(4.90±0.22)×109/L,模型+自拟方组(10只)(0.40±0.11)×109/L;14 d后模型+八珍组(8只)(0.49±0.10)×109/L,模型+六味组(9只)(0.60±0.15)×109/L,模型+自拟方组(7只)(1.26±0.05)×109/L;30 d后模型+八珍组(8只)(4.33±0.77)×109/L,模型+六味组(9只)(3.84 ±0.32)×109/L,模型+自拟方组(7只)(3.41±0.51)×109/L.中性粒细胞计数动态变化:照射1 d后辐射模型组(10只)(95.40±0.00)％,模型+八珍组(10只)(95.55±1.48)％,模型+六味组(10只)(86.30±1.42)％,模型+自拟方组(10只)(77.75±1.92)％;14 d后辐射模型组(5只)(46.45±3.89)％,模型+八珍组(8只)(47.07±6.67)％,模型+六味组(9只)(43.10±6.79)％,模型+自拟方组(7只)(32.00±1.10)％.经60 Coγ射线全身照射后实验大鼠机体显示出现炎症反应,免疫力降低,白细胞降低至正常值的1/10,中性粒细胞为正常的300％～ 400％.电镜观察经辐射大鼠骨髓结构较空白对照组明显塌陷,排列紊乱,部分黏膜脱落,中性粒细胞明显增多.结论 放射线可对机体造成血常规及骨髓结构改变,而中药方剂六味地黄丸具有较好的辐射防护效应,对急性重度和极重度骨髓放射病能起到预防和治疗作用.抗辐射新方对机体造血系统可起到有效防护及血常规再生功效,在中药对辐射造血系统损伤有良性干预作用.
目的 觀察不同方藥對輻射造血繫統大鼠的榦預作用.方法 將150隻健康Wistar雄性大鼠完全隨機分為空白對照組、輻射模型組、模型+八珍組、模型+六味組和模型+自擬方組,每組30隻.除空白對照組外,其餘4組均分彆施予60Coγ射線全身照射,吸收劑量分彆為7 Gy.分彆于照射後第1、14、30天取材,每組每次10隻,10％水閤氯醛腹腔痳醉,腹主動脈採血進行血常規測定,觀察外週血網織紅細胞微覈;取大鼠股骨標本,製作骨髓切片,電鏡觀察骨髓標本病理形態學的變化,分析輻射對造血繫統損傷及中藥對輻射損傷榦預作用.結果 經放射線照射後,各實驗組白細胞均下降,中性粒細胞比值上升.白細胞計數動態變化:照射1d後輻射模型組為(10隻)(1.04±0.00)×109/L,模型+八珍組為(10隻)(1.80±0.05)×109/L,模型+六味組(10隻)(4.90±0.22)×109/L,模型+自擬方組(10隻)(0.40±0.11)×109/L;14 d後模型+八珍組(8隻)(0.49±0.10)×109/L,模型+六味組(9隻)(0.60±0.15)×109/L,模型+自擬方組(7隻)(1.26±0.05)×109/L;30 d後模型+八珍組(8隻)(4.33±0.77)×109/L,模型+六味組(9隻)(3.84 ±0.32)×109/L,模型+自擬方組(7隻)(3.41±0.51)×109/L.中性粒細胞計數動態變化:照射1 d後輻射模型組(10隻)(95.40±0.00)％,模型+八珍組(10隻)(95.55±1.48)％,模型+六味組(10隻)(86.30±1.42)％,模型+自擬方組(10隻)(77.75±1.92)％;14 d後輻射模型組(5隻)(46.45±3.89)％,模型+八珍組(8隻)(47.07±6.67)％,模型+六味組(9隻)(43.10±6.79)％,模型+自擬方組(7隻)(32.00±1.10)％.經60 Coγ射線全身照射後實驗大鼠機體顯示齣現炎癥反應,免疫力降低,白細胞降低至正常值的1/10,中性粒細胞為正常的300％～ 400％.電鏡觀察經輻射大鼠骨髓結構較空白對照組明顯塌陷,排列紊亂,部分黏膜脫落,中性粒細胞明顯增多.結論 放射線可對機體造成血常規及骨髓結構改變,而中藥方劑六味地黃汍具有較好的輻射防護效應,對急性重度和極重度骨髓放射病能起到預防和治療作用.抗輻射新方對機體造血繫統可起到有效防護及血常規再生功效,在中藥對輻射造血繫統損傷有良性榦預作用.
목적 관찰불동방약대복사조혈계통대서적간예작용.방법 장150지건강Wistar웅성대서완전수궤분위공백대조조、복사모형조、모형+팔진조、모형+륙미조화모형+자의방조,매조30지.제공백대조조외,기여4조균분별시여60Coγ사선전신조사,흡수제량분별위7 Gy.분별우조사후제1、14、30천취재,매조매차10지,10％수합록철복강마취,복주동맥채혈진행혈상규측정,관찰외주혈망직홍세포미핵;취대서고골표본,제작골수절편,전경관찰골수표본병리형태학적변화,분석복사대조혈계통손상급중약대복사손상간예작용.결과 경방사선조사후,각실험조백세포균하강,중성립세포비치상승.백세포계수동태변화:조사1d후복사모형조위(10지)(1.04±0.00)×109/L,모형+팔진조위(10지)(1.80±0.05)×109/L,모형+륙미조(10지)(4.90±0.22)×109/L,모형+자의방조(10지)(0.40±0.11)×109/L;14 d후모형+팔진조(8지)(0.49±0.10)×109/L,모형+륙미조(9지)(0.60±0.15)×109/L,모형+자의방조(7지)(1.26±0.05)×109/L;30 d후모형+팔진조(8지)(4.33±0.77)×109/L,모형+륙미조(9지)(3.84 ±0.32)×109/L,모형+자의방조(7지)(3.41±0.51)×109/L.중성립세포계수동태변화:조사1 d후복사모형조(10지)(95.40±0.00)％,모형+팔진조(10지)(95.55±1.48)％,모형+륙미조(10지)(86.30±1.42)％,모형+자의방조(10지)(77.75±1.92)％;14 d후복사모형조(5지)(46.45±3.89)％,모형+팔진조(8지)(47.07±6.67)％,모형+륙미조(9지)(43.10±6.79)％,모형+자의방조(7지)(32.00±1.10)％.경60 Coγ사선전신조사후실험대서궤체현시출현염증반응,면역력강저,백세포강저지정상치적1/10,중성립세포위정상적300％～ 400％.전경관찰경복사대서골수결구교공백대조조명현탑함,배렬문란,부분점막탈락,중성립세포명현증다.결론 방사선가대궤체조성혈상규급골수결구개변,이중약방제륙미지황환구유교호적복사방호효응,대급성중도화겁중도골수방사병능기도예방화치료작용.항복사신방대궤체조혈계통가기도유효방호급혈상규재생공효,재중약대복사조혈계통손상유량성간예작용.
Objective To observe the intervention of Chinese medicine in hematopoietic system in rats caused by radiation.Methods Totally 150 healthy male Wister rats were divided randomly into 5 groups:normal group,radiation model group,radiation bazhen medicine group,radiation liuwei medicine group and radiation self-side medicine group,with 30 rats in each group.Except blank group,the remaining four groups received 7 Gy of 60Co γ-ray irradiation.After irradiation,the abdominal aortic blood were collected and determined using automatic biochemical analyzer; peripheral blood micronucleated reticulocye was observed; bone marrow specimens of pathological morphological changes and pathological changes were observed using electron microscopy.Results Afterradiation,the white blood cells of each experimental group were decreased and neutrophil ratio increased.After 60Co γ-ray irradiation,the bodies showed inflammatory response.The apparent collapse was observed in rat by radiation in electron microscope.Neutrophils increased significantly.Conclusions Radiation can cause the change of hemogram and bone marrow of body structure.The prescription of traditional Chinese medicine six taste glutinous rehmannia pill has good radiation protection effect on severe acute radiation sickness and plays a role in the prevention and treatment.Prescriptions of anti-radiation from can provide effective protection on hematopoiesis system and promote blood regeneration.
