中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
12期
1738-1739
,共2页
葛淑芝%刘慧颖%安玉萍%赵庭雪%刘印华%王月%张庆波
葛淑芝%劉慧穎%安玉萍%趙庭雪%劉印華%王月%張慶波
갈숙지%류혜영%안옥평%조정설%류인화%왕월%장경파
食品添加剂%酵母多糖%细胞增殖活性%细胞亚群
食品添加劑%酵母多糖%細胞增殖活性%細胞亞群
식품첨가제%효모다당%세포증식활성%세포아군
Food additives%Yeast polysaccharide%Cell proliferation%Cell subsets
目的 从免疫学角度探讨酵母多糖对机体免疫功能的影响,从而为食品添加剂产业提供新的产品.方法 分离提取小鼠的脾淋巴细胞,加入不同浓度酵母多糖(0.00、0.25、0.50、1.00、2.00、4.00 g/L)后进行培养,对其进行增殖活性、白细胞介素2(IL-2)分泌量的测定以及细胞表型检测,并与对照组比较.结果 加入0.50、1.00、2.00、4.00 g/L酵母多糖培养3、6、9d后,小鼠脾淋巴细胞增殖呈上升趋势,各组促增效果均明显超过0.00 g/L组(P＜0.05或P＜0.01).加入0.25、0.50、1.00、2.00、4.00 g/L酵母多糖培养.9d后,各浓度组脾淋巴细胞IL-2的分泌量均高于0.00 g/L组[(9.4±1.3)、(13.4±1.2)、(13.2±1.2)、(15.6±3.6)、(10.8±1.3) μg/L比(7.3±1.1)μg/L,P＜0.05].培养6d的2.00 g/L组CD3+、CD19+标记细胞百分比分别为(9.14±0.31)％、(4.47±0.55)％,明显高于0.00 g/L组[分别为(5.01 ±0.74)％、(2.73±0.22)％,P＜0.01].结论 酵母多糖可增强机体的免疫功能.
目的 從免疫學角度探討酵母多糖對機體免疫功能的影響,從而為食品添加劑產業提供新的產品.方法 分離提取小鼠的脾淋巴細胞,加入不同濃度酵母多糖(0.00、0.25、0.50、1.00、2.00、4.00 g/L)後進行培養,對其進行增殖活性、白細胞介素2(IL-2)分泌量的測定以及細胞錶型檢測,併與對照組比較.結果 加入0.50、1.00、2.00、4.00 g/L酵母多糖培養3、6、9d後,小鼠脾淋巴細胞增殖呈上升趨勢,各組促增效果均明顯超過0.00 g/L組(P＜0.05或P＜0.01).加入0.25、0.50、1.00、2.00、4.00 g/L酵母多糖培養.9d後,各濃度組脾淋巴細胞IL-2的分泌量均高于0.00 g/L組[(9.4±1.3)、(13.4±1.2)、(13.2±1.2)、(15.6±3.6)、(10.8±1.3) μg/L比(7.3±1.1)μg/L,P＜0.05].培養6d的2.00 g/L組CD3+、CD19+標記細胞百分比分彆為(9.14±0.31)％、(4.47±0.55)％,明顯高于0.00 g/L組[分彆為(5.01 ±0.74)％、(2.73±0.22)％,P＜0.01].結論 酵母多糖可增彊機體的免疫功能.
목적 종면역학각도탐토효모다당대궤체면역공능적영향,종이위식품첨가제산업제공신적산품.방법 분리제취소서적비림파세포,가입불동농도효모다당(0.00、0.25、0.50、1.00、2.00、4.00 g/L)후진행배양,대기진행증식활성、백세포개소2(IL-2)분비량적측정이급세포표형검측,병여대조조비교.결과 가입0.50、1.00、2.00、4.00 g/L효모다당배양3、6、9d후,소서비림파세포증식정상승추세,각조촉증효과균명현초과0.00 g/L조(P＜0.05혹P＜0.01).가입0.25、0.50、1.00、2.00、4.00 g/L효모다당배양.9d후,각농도조비림파세포IL-2적분비량균고우0.00 g/L조[(9.4±1.3)、(13.4±1.2)、(13.2±1.2)、(15.6±3.6)、(10.8±1.3) μg/L비(7.3±1.1)μg/L,P＜0.05].배양6d적2.00 g/L조CD3+、CD19+표기세포백분비분별위(9.14±0.31)％、(4.47±0.55)％,명현고우0.00 g/L조[분별위(5.01 ±0.74)％、(2.73±0.22)％,P＜0.01].결론 효모다당가증강궤체적면역공능.
Objective To investigate the effects of yeast polysaccharides on immune function of mice; to provide a new product for food additive industry.Methods In vitro,spleen lymphocytes of mouse were separated,extracted and cultured with yeast polysaccharides (0.00,0.25,0.50,1.00,2.00,4.00 g,/L) for 3,6,9 d.Then the proliferation and secretion amount of inter/eukin 2(IL-2) were detected and cell phenotypes were analyzed.In vivo,carps of the experimental group were fed with yeast polysaccharides.The feeding time was recorded and compared.Results The proliferation of spleen lymphocytes cultured with 0.50,1.00,2.00,4.00 g/L yeast polysaccharide for 3,6,9 d was significantly higher than that of 0.00 g/L (P ＜ 0.05) ; the secretion of IL-2 in 0.25,0.50,1.00,2.00,4.00 g/L yeast polysaccharid for 9 d was notably increased compared with the 0.00 g/L yeast polysaccharid [(9.4±1.3),(13.4±1.2),(13.2 ±1.2),(15.6±3.6),(10.8 ±1.3)μg/L vs (7.3 ± 1.1)μg/L,P＜0.05];when yeast polysaccharid was 2.00 g/L for 6 d,the amount of T/B cells were (9.14 ± 0.31) ％,(4.47 ± 0.55) ％,which was significantly higher than that of 2.00 g/L yeast polysaccharid [(5.01 ± 0.74) ％,(2.73 ± 0.22) ％,P ＜0.01].Yeast polysaccharid enhanced feeding speed of carps [(10 ± 5) min vs (23 ± 13) min,P ＜ 0.01].Conclusion Yeast polysaccharid can be used as food additives to enhance immune function and appetite.
