中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
1期
118-120
,共3页
王晶%方芳%雷力力%孟繁颖%方洪壮
王晶%方芳%雷力力%孟繁穎%方洪壯
왕정%방방%뢰력력%맹번영%방홍장
复方黄白胶囊%黄酮%含量测定
複方黃白膠囊%黃酮%含量測定
복방황백효낭%황동%함량측정
Compound huangbai capsules%Flavones%Content determination
目的 建立一测多评测定复方黄白胶囊中4种黄酮成分(黄芩苷、汉黄芩苷、黄芩素和汉黄芩素)的方法.方法 采用斜率校正法,以黄芩苷作为内参物,计算汉黄芩苷、黄芩素和汉黄芩素与黄芩苷的相对校正因子;分别用一测多评法和外标法测定复方黄白胶囊中4种黄酮成分的含量.结果 黄芩苷、汉黄芩苷、黄芩素及汉黄芩素的色谱保留时间分别为14.80、28.58、37.76及47.52.阴性对照在相应位置处未见色谱峰,样品色谱图中黄芩苷、汉黄芩苷、黄芩素及汉黄芩素色谱峰的纯度因子分别为998.8、995.2、989.6及988.5.汉黄芩苷、黄芩素及汉黄芩素与黄芩苷间的相对校正因子分别为1.396、1.808和2.010.复方黄白胶囊中4种黄酮成分采用校正因子计算的含量值与外标法实测值之间无明显差异.黄芩苷、汉黄芩苷、黄芩素及汉黄芩素的加样回收率平均值分别为99.5％、99.7％、100.5％、99.4％,相对标准偏差分别为1.3％、0.9％、1.6％及1.1％(n=6).本研究表明一测多评法测定黄芩苷、汉黄芩苷、黄芩素及汉黄芩素方法专属性、精密度、稳定性、重复性和回收率均良好.结论 一测多评法可用于复方黄白胶囊中4种黄酮成分的定量分析.
目的 建立一測多評測定複方黃白膠囊中4種黃酮成分(黃芩苷、漢黃芩苷、黃芩素和漢黃芩素)的方法.方法 採用斜率校正法,以黃芩苷作為內參物,計算漢黃芩苷、黃芩素和漢黃芩素與黃芩苷的相對校正因子;分彆用一測多評法和外標法測定複方黃白膠囊中4種黃酮成分的含量.結果 黃芩苷、漢黃芩苷、黃芩素及漢黃芩素的色譜保留時間分彆為14.80、28.58、37.76及47.52.陰性對照在相應位置處未見色譜峰,樣品色譜圖中黃芩苷、漢黃芩苷、黃芩素及漢黃芩素色譜峰的純度因子分彆為998.8、995.2、989.6及988.5.漢黃芩苷、黃芩素及漢黃芩素與黃芩苷間的相對校正因子分彆為1.396、1.808和2.010.複方黃白膠囊中4種黃酮成分採用校正因子計算的含量值與外標法實測值之間無明顯差異.黃芩苷、漢黃芩苷、黃芩素及漢黃芩素的加樣迴收率平均值分彆為99.5％、99.7％、100.5％、99.4％,相對標準偏差分彆為1.3％、0.9％、1.6％及1.1％(n=6).本研究錶明一測多評法測定黃芩苷、漢黃芩苷、黃芩素及漢黃芩素方法專屬性、精密度、穩定性、重複性和迴收率均良好.結論 一測多評法可用于複方黃白膠囊中4種黃酮成分的定量分析.
목적 건립일측다평측정복방황백효낭중4충황동성분(황금감、한황금감、황금소화한황금소)적방법.방법 채용사솔교정법,이황금감작위내삼물,계산한황금감、황금소화한황금소여황금감적상대교정인자;분별용일측다평법화외표법측정복방황백효낭중4충황동성분적함량.결과 황금감、한황금감、황금소급한황금소적색보보류시간분별위14.80、28.58、37.76급47.52.음성대조재상응위치처미견색보봉,양품색보도중황금감、한황금감、황금소급한황금소색보봉적순도인자분별위998.8、995.2、989.6급988.5.한황금감、황금소급한황금소여황금감간적상대교정인자분별위1.396、1.808화2.010.복방황백효낭중4충황동성분채용교정인자계산적함량치여외표법실측치지간무명현차이.황금감、한황금감、황금소급한황금소적가양회수솔평균치분별위99.5％、99.7％、100.5％、99.4％,상대표준편차분별위1.3％、0.9％、1.6％급1.1％(n=6).본연구표명일측다평법측정황금감、한황금감、황금소급한황금소방법전속성、정밀도、은정성、중복성화회수솔균량호.결론 일측다평법가용우복방황백효낭중4충황동성분적정량분석.
Objective To establish a quantitative method for the quantitative analysis of multiple components with a single maker (QAMS) to determine the content of four flavones in compound huangbai capsules.Methods Baicalin was used as the internal reference.The relative correlation factors (RCFs) of wogonoside,baicalein and wogonin to baicalin were calculated by slope correction method.The contents of these four flavones were determined by the external standard method and QAMS respectively.Results The chromatographic retention times of baicalin,wogonoside,baicalein and wogonin were 14.80,28.58,37.76 and 47.52,respectively.Purity factor of baicalin,wogonoside,baicalein and wogonin were 998.8,995.2,989.6 and 988.5,respectively.The RCFs of wogonoside,baicalein and wogonin to baicalin were 1.369,1.808 and 2.010 respectively.No significant differences between the quantitative results of the two methods were observed.The average recoveries of baicalin,wogonoside,baicaleiu and wogonin were 99.5％,99.7％,100.5％,99.4％ and relative standard deviations were 1.3％,0.9％,1.6％ and 1.1％ (n =6).Conclusion The established QAMS method can be applied for the quantitative determination of baicalin,wogonoside,baicalein and wogonin in compound huangbai capsules.
