中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
6期
778-782
,共5页
聂晓敏%苏利霄%周雅婧%赵迎新%史冬梅%刘宇扬%周志明%周玉杰
聶曉敏%囌利霄%週雅婧%趙迎新%史鼕梅%劉宇颺%週誌明%週玉傑
섭효민%소리소%주아청%조영신%사동매%류우양%주지명%주옥걸
冠状动脉疾病%微小RNA-221%微小RNA-222%侧支循环
冠狀動脈疾病%微小RNA-221%微小RNA-222%側支循環
관상동맥질병%미소RNA-221%미소RNA-222%측지순배
Coronary artery disease%MicroRNA-221%MicroRNA-222%Collateral circulation
目的 观察冠心病患者血浆中微小RNA-221和微小RNA-222水平与冠状动脉侧支循环形成的关系.方法 连续入选在首都医科大学附属北京安贞医院心内科接受选择性冠状动脉造影检查,证实左前降支、左回旋支或右冠状动脉中单支血管狭窄≥95％的冠心病患者120例,根据Rentrop分级将患者分成2组:侧支良好组(Rentrop ≥2级)(n=64),侧支不良组(Rentrop≤1级)(n=56).采用实时荧光定量-聚合酶链反应(qRT-PCR)检测血浆微小RNA-221和微小RNA-222的表达水平,酶联免疫吸附试验(ELISA)检测血清中c-kit和内皮源性一氧化氮合酶(eNOS)的浓度.采用Pearson相关分析和Logistic回归进行相关性分析.结果 侧支不良组血浆中微小RNA-221和微小RNA-222水平明显高于侧支良好组(0.25 ±0.04比0.13 ±0.03;0.21 ±0.06比0.12 ±0.02,P=0.001和P=0.003).侧支不良组血清c-kit和eNOS水平低于侧支良好组[(3.8±1.3)μg/L 比(6.2 ± 2.3)μg/L;(18.7±5.5) μg/L比(24.9±8.0) μg/L,二者均P＜0.05].侧支良好组和侧支不良组血液中微小RNA-221和微小RNA-222与c-kit和eNOS明显负相关[(侧支良好组微小RNA-221与c-kit:r=-0.581,P=0.012;微小RNA-221与eNOS:r=-0.534,P=0.019;侧支不良组微小RNA-221与c-kit:r=-0.653,P=0.021;微小RNA-221与eNOS:r=-0.061,P=0.028)、(侧支良好组微小RNA-222与c-kit:r=-0.495,P=0.004;微小RNA-222与eNOS:r=-0.483,P=0.022;侧支不良组侧支良好组微小RNA-222与c-kit:r=-0.638,P=0.035;微小RNA-222与eNOS:r=-0.623,P=0.015],而健康对照组中微小RNA-221/微小RNA-222与c.-kit和eNOS没有明显相关性(微小RNA-221与c-kit:r=-0.075,P=0.676;微小RNA-222与c-kit:r=-0.058,P=0.722;微小RNA-221与eNOS:r=-0.084,P=0.342;微小RNA-222与eNOS:r=-0.045,P=0.812).微小RNA-221和微小RNA-222是冠状动脉侧支循环形成的独立预测因子[比值比(OR) =2.246,P=0.012;OR=2.373,P=0.003)].结论 血浆微小RNA-221和微小RNA-222水平与冠状动脉侧支循环形成明显负相关,是冠状动脉侧支循环形成的独立预测因素.
目的 觀察冠心病患者血漿中微小RNA-221和微小RNA-222水平與冠狀動脈側支循環形成的關繫.方法 連續入選在首都醫科大學附屬北京安貞醫院心內科接受選擇性冠狀動脈造影檢查,證實左前降支、左迴鏇支或右冠狀動脈中單支血管狹窄≥95％的冠心病患者120例,根據Rentrop分級將患者分成2組:側支良好組(Rentrop ≥2級)(n=64),側支不良組(Rentrop≤1級)(n=56).採用實時熒光定量-聚閤酶鏈反應(qRT-PCR)檢測血漿微小RNA-221和微小RNA-222的錶達水平,酶聯免疫吸附試驗(ELISA)檢測血清中c-kit和內皮源性一氧化氮閤酶(eNOS)的濃度.採用Pearson相關分析和Logistic迴歸進行相關性分析.結果 側支不良組血漿中微小RNA-221和微小RNA-222水平明顯高于側支良好組(0.25 ±0.04比0.13 ±0.03;0.21 ±0.06比0.12 ±0.02,P=0.001和P=0.003).側支不良組血清c-kit和eNOS水平低于側支良好組[(3.8±1.3)μg/L 比(6.2 ± 2.3)μg/L;(18.7±5.5) μg/L比(24.9±8.0) μg/L,二者均P＜0.05].側支良好組和側支不良組血液中微小RNA-221和微小RNA-222與c-kit和eNOS明顯負相關[(側支良好組微小RNA-221與c-kit:r=-0.581,P=0.012;微小RNA-221與eNOS:r=-0.534,P=0.019;側支不良組微小RNA-221與c-kit:r=-0.653,P=0.021;微小RNA-221與eNOS:r=-0.061,P=0.028)、(側支良好組微小RNA-222與c-kit:r=-0.495,P=0.004;微小RNA-222與eNOS:r=-0.483,P=0.022;側支不良組側支良好組微小RNA-222與c-kit:r=-0.638,P=0.035;微小RNA-222與eNOS:r=-0.623,P=0.015],而健康對照組中微小RNA-221/微小RNA-222與c.-kit和eNOS沒有明顯相關性(微小RNA-221與c-kit:r=-0.075,P=0.676;微小RNA-222與c-kit:r=-0.058,P=0.722;微小RNA-221與eNOS:r=-0.084,P=0.342;微小RNA-222與eNOS:r=-0.045,P=0.812).微小RNA-221和微小RNA-222是冠狀動脈側支循環形成的獨立預測因子[比值比(OR) =2.246,P=0.012;OR=2.373,P=0.003)].結論 血漿微小RNA-221和微小RNA-222水平與冠狀動脈側支循環形成明顯負相關,是冠狀動脈側支循環形成的獨立預測因素.
목적 관찰관심병환자혈장중미소RNA-221화미소RNA-222수평여관상동맥측지순배형성적관계.방법 련속입선재수도의과대학부속북경안정의원심내과접수선택성관상동맥조영검사,증실좌전강지、좌회선지혹우관상동맥중단지혈관협착≥95％적관심병환자120례,근거Rentrop분급장환자분성2조:측지량호조(Rentrop ≥2급)(n=64),측지불량조(Rentrop≤1급)(n=56).채용실시형광정량-취합매련반응(qRT-PCR)검측혈장미소RNA-221화미소RNA-222적표체수평,매련면역흡부시험(ELISA)검측혈청중c-kit화내피원성일양화담합매(eNOS)적농도.채용Pearson상관분석화Logistic회귀진행상관성분석.결과 측지불량조혈장중미소RNA-221화미소RNA-222수평명현고우측지량호조(0.25 ±0.04비0.13 ±0.03;0.21 ±0.06비0.12 ±0.02,P=0.001화P=0.003).측지불량조혈청c-kit화eNOS수평저우측지량호조[(3.8±1.3)μg/L 비(6.2 ± 2.3)μg/L;(18.7±5.5) μg/L비(24.9±8.0) μg/L,이자균P＜0.05].측지량호조화측지불량조혈액중미소RNA-221화미소RNA-222여c-kit화eNOS명현부상관[(측지량호조미소RNA-221여c-kit:r=-0.581,P=0.012;미소RNA-221여eNOS:r=-0.534,P=0.019;측지불량조미소RNA-221여c-kit:r=-0.653,P=0.021;미소RNA-221여eNOS:r=-0.061,P=0.028)、(측지량호조미소RNA-222여c-kit:r=-0.495,P=0.004;미소RNA-222여eNOS:r=-0.483,P=0.022;측지불량조측지량호조미소RNA-222여c-kit:r=-0.638,P=0.035;미소RNA-222여eNOS:r=-0.623,P=0.015],이건강대조조중미소RNA-221/미소RNA-222여c.-kit화eNOS몰유명현상관성(미소RNA-221여c-kit:r=-0.075,P=0.676;미소RNA-222여c-kit:r=-0.058,P=0.722;미소RNA-221여eNOS:r=-0.084,P=0.342;미소RNA-222여eNOS:r=-0.045,P=0.812).미소RNA-221화미소RNA-222시관상동맥측지순배형성적독립예측인자[비치비(OR) =2.246,P=0.012;OR=2.373,P=0.003)].결론 혈장미소RNA-221화미소RNA-222수평여관상동맥측지순배형성명현부상관,시관상동맥측지순배형성적독립예측인소.
Objective To study the relationship between plasma microRNA-221 and microRNA-222 (miR-221/222) levels and coronary collateral circulation (CCC) formation in patients with severely narrowed coronary arteries.Methods A total of 120 patients with coronary heart disease in Beijing Anzhen Hospital who had 95％ or greater of stenosis in one epicardial coronary artery were enrolled consecutively.They were divided into two groups according to Rentrop grades:patients with grade 2 and 3 collateral development were regarded as good CCC group (n =64) and patients with grade 0-1 collateral development were regarded as poor CCC group (n =56).Plasma miR-221/222 was measured by real-time quantitative-polymerase chain reaction (qRT-PCR) and serum c-kit and endothelial nitric oxide synthase (eNOS) were evaluated by enzyme linked immunosorbent assay (ELISA).The data were statistically analyzed by Pearson and Logistic correlation analysis.Results Compared with poor CCC patients,patients with good CCC had lower miR-221/222 levels [0.25 ± 0.04 vs 0.13 ± 0.03,0.21 ± 0.06 vs 0.12 ± 0.02,P =0.001,P =0.003] and higher c-kit and eNOS levels [(3.8 ±1.3) μg/L vs (6.2 ±2.3) μg/L,and (18.7 ±5.5) μg/L vs (24.9 ±8.0) μg/L].In CAD patients with good CCC and poor CCC,the miR-221/222 levels were negatively correlated to the c-kit and eNOS expression [Good CCC group:miR-221 and c-kit expression(r =-0.581,P =0.012),miR-221 and eNOS (r =-0.534,P =0.019) ; Poor CCCgroup:miR-221 and c-kit expression(r=-0.653,P=0.021) miR-221 and eNOS (r=-0.661,P=0.028).Good CCC group:miR-221 and c-kit (r =-0.638,P =0.035); miR-222 and c-kit(r =-0.495,P =0.004) ;miR-222 and eNOS(r =-0.483,P =0.022) ; Poor CCC group:miR-222 and eNOS(r =-0.623,P =0.015).Of the healthy people,there were no significant relationship among miR-221/222 and c-kit or eNOS(miR-221 and c-kit:r =-0.075,P =0.676; miR-222 and c-kit:r =-0.058,P =0.722; miR-221and eNOS:r =-0.084,P =0.342; miR222 and eNOS:r =-0.045,P =0.812).In multivariate analysis,plasma miR-221/222 was an independent predictor of collateral development (OR =2.246,P =0.012 and OR =2.373,P =0.003).Conclusion Plasma miR-221/222 levels are closely related to the CCC formation and are independent predictors of CCC development.
