中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
6期
872-877
,共6页
宫颈癌%侧群细胞%肿瘤干细胞%微小RNA
宮頸癌%側群細胞%腫瘤榦細胞%微小RNA
궁경암%측군세포%종류간세포%미소RNA
Cervical cancer%Side population cell%Cancer stem cells%microRNA
目的 筛选人宫颈癌侧群细胞差异表达的微小RNA(microRNA).方法 选取16例行宫颈鳞癌切除的手术标本,进行宫颈癌细胞原代培养,流式细胞仪分选侧群细胞,对比侧群细胞和非侧群细胞的体外自我更新能力和肿瘤形成能力.分别提取侧群细胞和非侧群细胞的总RNA,用microRNA芯片分别检测侧群细胞和非侧群细胞的microRNA表达谱并进行分析,筛选出差异表达的microRNA.结果 在原代培养的16株宫颈癌细胞中均分选出侧群细胞,比例占(1.67 ±0.94)％.培养14 d后,侧群细胞的克隆形成率为0.51 ±0.07,而非侧群细胞的克隆形成率仅为0.05±0.02,侧群细胞和非侧群细胞的克隆形成率比较差异有统计学意义(t=2.828,P＜0.01).仅1 × 104个侧群细胞即可形成肿瘤,而至少1 ×106个非侧群细胞才能形成肿瘤.microRNA芯片筛选出表达差异在10倍以上的有16条microRNA,其中3条microRNA上调,13条microRNA下调;差异表达超过共同2倍的microRNA有7条,其中3条microRNA上调,4条microRNA下调.结论 人宫颈癌侧群细胞具有肿瘤干细胞特性,人宫颈癌侧群细胞表达一些特异的microRNA.
目的 篩選人宮頸癌側群細胞差異錶達的微小RNA(microRNA).方法 選取16例行宮頸鱗癌切除的手術標本,進行宮頸癌細胞原代培養,流式細胞儀分選側群細胞,對比側群細胞和非側群細胞的體外自我更新能力和腫瘤形成能力.分彆提取側群細胞和非側群細胞的總RNA,用microRNA芯片分彆檢測側群細胞和非側群細胞的microRNA錶達譜併進行分析,篩選齣差異錶達的microRNA.結果 在原代培養的16株宮頸癌細胞中均分選齣側群細胞,比例佔(1.67 ±0.94)％.培養14 d後,側群細胞的剋隆形成率為0.51 ±0.07,而非側群細胞的剋隆形成率僅為0.05±0.02,側群細胞和非側群細胞的剋隆形成率比較差異有統計學意義(t=2.828,P＜0.01).僅1 × 104箇側群細胞即可形成腫瘤,而至少1 ×106箇非側群細胞纔能形成腫瘤.microRNA芯片篩選齣錶達差異在10倍以上的有16條microRNA,其中3條microRNA上調,13條microRNA下調;差異錶達超過共同2倍的microRNA有7條,其中3條microRNA上調,4條microRNA下調.結論 人宮頸癌側群細胞具有腫瘤榦細胞特性,人宮頸癌側群細胞錶達一些特異的microRNA.
목적 사선인궁경암측군세포차이표체적미소RNA(microRNA).방법 선취16례행궁경린암절제적수술표본,진행궁경암세포원대배양,류식세포의분선측군세포,대비측군세포화비측군세포적체외자아경신능력화종류형성능력.분별제취측군세포화비측군세포적총RNA,용microRNA심편분별검측측군세포화비측군세포적microRNA표체보병진행분석,사선출차이표체적microRNA.결과 재원대배양적16주궁경암세포중균분선출측군세포,비례점(1.67 ±0.94)％.배양14 d후,측군세포적극륭형성솔위0.51 ±0.07,이비측군세포적극륭형성솔부위0.05±0.02,측군세포화비측군세포적극륭형성솔비교차이유통계학의의(t=2.828,P＜0.01).부1 × 104개측군세포즉가형성종류,이지소1 ×106개비측군세포재능형성종류.microRNA심편사선출표체차이재10배이상적유16조microRNA,기중3조microRNA상조,13조microRNA하조;차이표체초과공동2배적microRNA유7조,기중3조microRNA상조,4조microRNA하조.결론 인궁경암측군세포구유종류간세포특성,인궁경암측군세포표체일사특이적microRNA.
Objective To screen the expression of microRNA of human cervical cancer side population (SP) cells.Methods The human cervical cancer cells were obtained from fresh human cervical cancer tissue among 16 patients who were diagnosed of cervical cancer.Flow cytometry and Hoechst33342 dye efflux assay were used to isolate SP cells and NSP(non side population) cells from primary cervical cancer cells.Self-renewal and tumorigenic ability of SP and NSP cells were compared in vitro and in vivo.MicroRNA was isolated from cervical cancer SP an NSP cells.Hybridization was carried out on Sanger miRBase 17.0 Arrays.Screening of microRNA between SP and NSP cells was undertaken Microarray Software.Results The SP cells demonstrated a higher cloning ability in vitro and a stronger tumorigenesis ability compared with NSP cells in nude mice.16 microRNAs expression were found in SP cells,10 times more than that in NSP cells.Three microRNAs were up-regulated and 13microRNAs were down-regulated in SP cells.3 microRNAs were up-regulated and 4 microRNAs were down-regulated in SP cells.Conclusions Human cervical cancer SP cells have the characters of cancer stem cells.Some specific microRNAs are found between human cervical cancer SP cells and NSP cells.
