中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
6期
890-893
,共4页
张小团%肖勇健%刘卓然%刘佳倪
張小糰%肖勇健%劉卓然%劉佳倪
장소단%초용건%류탁연%류가예
EB病毒%基因分型%儿童%咽拭子
EB病毒%基因分型%兒童%嚥拭子
EB병독%기인분형%인동%인식자
Epstein-Barr virus%Genotype%Child%Nasopharynx
目的 了解湖南省株洲、湘潭和衡阳地区儿童鼻咽部EB病毒的感染状况及其基因型,为EB病毒的治疗、预防和临床检验提供指导.方法 从株洲、湘潭和衡阳部分医院收集疑似EB病毒感染的儿童咽拭子标本406份,采用荧光定量聚合酶联反应(FQ-PCR)检测标本中的EB病毒-DNA;阳性标本分别用特异性引物从中扩增3个目的基因片段,其中PCR扩增的EBNA-3C片段直接电泳以分析1/2分型,BamHIF片段和BamHI WI/I1交界区基因片段采用PCR-RFLP分析鉴定F/f和C/D分型,测序和Blast比对验证分型结果.结果 从406份标本中共检出EB病毒-DNA阳性株159份,株洲74份(38.5％),其中1型72份(97.3％),2型2份(2.7％);湘潭42份(38.9％),1型40份(95.2％),2型2份(4.8％);衡阳43份(40.6％),1型43份(100％),2型未发现.从159份阳性株中随机扩增了73份做F/f分型,68份做C/D分型,共检测到F型73份,C型68份,3个地区均未检测到f型和D型.结论 株洲,湘潭和衡阳儿童鼻咽部EB病毒感染的检出率分别为38.5％,38.9％,40.6％,且其主要感染的3种独立基因型以1、C、F型为主,3个地区间儿童鼻咽部EB病毒-DNA的检出率及其基因型的差异无统计学意义.
目的 瞭解湖南省株洲、湘潭和衡暘地區兒童鼻嚥部EB病毒的感染狀況及其基因型,為EB病毒的治療、預防和臨床檢驗提供指導.方法 從株洲、湘潭和衡暘部分醫院收集疑似EB病毒感染的兒童嚥拭子標本406份,採用熒光定量聚閤酶聯反應(FQ-PCR)檢測標本中的EB病毒-DNA;暘性標本分彆用特異性引物從中擴增3箇目的基因片段,其中PCR擴增的EBNA-3C片段直接電泳以分析1/2分型,BamHIF片段和BamHI WI/I1交界區基因片段採用PCR-RFLP分析鑒定F/f和C/D分型,測序和Blast比對驗證分型結果.結果 從406份標本中共檢齣EB病毒-DNA暘性株159份,株洲74份(38.5％),其中1型72份(97.3％),2型2份(2.7％);湘潭42份(38.9％),1型40份(95.2％),2型2份(4.8％);衡暘43份(40.6％),1型43份(100％),2型未髮現.從159份暘性株中隨機擴增瞭73份做F/f分型,68份做C/D分型,共檢測到F型73份,C型68份,3箇地區均未檢測到f型和D型.結論 株洲,湘潭和衡暘兒童鼻嚥部EB病毒感染的檢齣率分彆為38.5％,38.9％,40.6％,且其主要感染的3種獨立基因型以1、C、F型為主,3箇地區間兒童鼻嚥部EB病毒-DNA的檢齣率及其基因型的差異無統計學意義.
목적 료해호남성주주、상담화형양지구인동비인부EB병독적감염상황급기기인형,위EB병독적치료、예방화림상검험제공지도.방법 종주주、상담화형양부분의원수집의사EB병독감염적인동인식자표본406빈,채용형광정량취합매련반응(FQ-PCR)검측표본중적EB병독-DNA;양성표본분별용특이성인물종중확증3개목적기인편단,기중PCR확증적EBNA-3C편단직접전영이분석1/2분형,BamHIF편단화BamHI WI/I1교계구기인편단채용PCR-RFLP분석감정F/f화C/D분형,측서화Blast비대험증분형결과.결과 종406빈표본중공검출EB병독-DNA양성주159빈,주주74빈(38.5％),기중1형72빈(97.3％),2형2빈(2.7％);상담42빈(38.9％),1형40빈(95.2％),2형2빈(4.8％);형양43빈(40.6％),1형43빈(100％),2형미발현.종159빈양성주중수궤확증료73빈주F/f분형,68빈주C/D분형,공검측도F형73빈,C형68빈,3개지구균미검측도f형화D형.결론 주주,상담화형양인동비인부EB병독감염적검출솔분별위38.5％,38.9％,40.6％,차기주요감염적3충독립기인형이1、C、F형위주,3개지구간인동비인부EB병독-DNA적검출솔급기기인형적차이무통계학의의.
Objective To know the state and genotyping of Epstein-Barr virus (EBV) infection of nasopharyngeal swabs in children.Methods Totally 406 samples were detected for EBV-DNA by RQ-PCR.The samples were collected from nasopharyngeal swabs of children passibly suffering from Epstein-Barr Virus infection in Zhuzhou,Xiangtan and Hengyang cities in Hunan province in China.The analysis of genotypes was implemented on three regions of EBV genome; EBNA-3C,BamHI WI/I1and BamHI Fwere detected by the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results Totally 159 clinical strains were identified from children nasopharyngeal swabs.The EBV positive strains were detected in 74 of 192,42 of 108,43of 106 samples from Zhuzhou,Xiangtan and Hengyang,respectively.The genotype 1 was proven in 74/192,42/108,43/106 clinical samples in those areas,respectively.The frequencies of genotype F and genotype C were detected in all cases (100％).Conclusions The frequencies of EBV-DNA are 38.5％,38.9％ and 40.6％ ; genotypes 1,C and F are the predominant genotyping of EBV.The frequencies of EBV-DNA and the type 1/2,type C/D,type F/f are not significantly different.
