CAJ | 학술논문

目的观察针刺任脉、督脉及膀胱经对缺血缺氧性脑病模型幼鼠改善学习与记忆能力的影响.方法将新生7dSD幼鼠40只完全随机分为正常组、模型组、针刺治疗组及随机针刺组,每组10只.模型组、随机针刺组及针刺治疗组结扎左侧颈总动脉并实施缺氧处理,并在模型建立完成后12 h内行头部MRI扫描,观察模型建立成功与否以及累及范围,同时测定病灶中心的表观扩散系数(ADC)值.针刺治疗组及随机针刺组于造模后第1天开始取任督二脉及膀胱经行针刺治疗,连续28 d,每天1次.各组大鼠在针刺治疗后28 d,水迷宫实验观察针刺后模型动物的行为变化,分别观察穿越平台次数及在日的象限停留时间,并比较它们之间的差异.结果 MRI扫描提示模型建立成功,观看到缺血缺氧病灶累及皮质及海马等,测量建立模型后动物脑部病灶中心区ADC值为(3.35 ±0.09) ×10-4 mm2/s,低于重度缺血缺氧最终平均ADC值范围.在水迷宫行为学实验中,针刺治疗组在穿越平台次数及平台象限停留时间优于模型组及随机针刺组,差异均有统计学意义[穿越平台次数:(3.0±1.1)次比(1.2±1.1)次、(1.5±1.4)次;平台象限停留时间百分比:(38±4)％比(26±7)％、(21±10)％,均P＜0.05].结论针刺任督二脉及膀胱经对治疗缺血缺氧性脑损伤可对模型鼠学习与记忆能力下降有改善.
목적 관찰침자임맥、독맥급방광경대결혈결양성뇌병모형유서개선학습여기억능력적영향.방법 장신생7dSD유서40지완전수궤분위정상조、모형조、침자치료조급수궤침자조,매조10지.모형조、수궤침자조급침자치료조결찰좌측경총동맥병실시결양처리,병재모형건립완성후12 h내행두부MRI소묘,관찰모형건립성공여부이급루급범위,동시측정병조중심적표관확산계수(ADC)치.침자치료조급수궤침자조우조모후제1천개시취임독이맥급방광경행침자치료,련속28 d,매천1차.각조대서재침자치료후28 d,수미궁실험관찰침자후모형동물적행위변화,분별관찰천월평태차수급재일적상한정류시간,병비교타문지간적차이.결과 MRI소묘제시모형건립성공,관간도결혈결양병조루급피질급해마등,측량건립모형후동물뇌부병조중심구ADC치위(3.35 ±0.09) ×10-4 mm2/s,저우중도결혈결양최종평균ADC치범위.재수미궁행위학실험중,침자치료조재천월평태차수급평태상한정류시간우우모형조급수궤침자조,차이균유통계학의의[천월평태차수:(3.0±1.1)차비(1.2±1.1)차、(1.5±1.4)차;평태상한정류시간백분비:(38±4)％비(26±7)％、(21±10)％,균P＜0.05].결론 침자임독이맥급방광경대치료결혈결양성뇌손상가대모형서학습여기억능력하강유개선.
Objective To observe the successful establishment ofhypoxic-ischemic encephalopathy(HIE) rat models and to detect the improvement of learning HIE rat models during acupuncture on conception vessel,governor vesse and bladder channel.Methods The animals were divided into 4 groups:the normal group,model group,acupuncture group and random acupuncture group.The model group,acupuncture group and control group of 7-day-old SD rats underwent left common carotid artery ligation.After the establishment of HIE rat models,all groups had the magnetic resonance imaging (MRI) scan within 12 hour,which could find out the HIE model was successful or not ; we observed the involving area and determinedthe apparent diffusion coefficient (ADC) of the lesion center.At the same time acupuncture was performed once a day on conception vessel,governor vessel and bladder channel.Normal group did not have surgery and acupuncture.Model group did not had acupuncture and the control group had acupuncture randomlv.The ability of learning and memory was assessed by Morris water maze 28 days after model establishment.The time of crossing the flat and the residence time in the target quadrant were observed and compared.Results MRI scan could observe the successful establishment of HIE which involved both cortex and hippocampus.After the establishment of HIE rat models,the ADC of the model animal central lesions was (3.35 ± 0.09) × 10-4 mm2/s,which was lower than the values range of ADC in the severe ischemia hypoxia.The time of crossing the flat and the residence time in the target quadrant in the acupuncture group were significantly different from those in the model group and the random acupuncture group [(3.0 ± 1.1) times vs (1.2±l.1)timesor (1.5±l.4)times;(37.9±3.9)％ vs (25.7 ±7.4)％ or (21.3 ±10.1)％,all P＜0.05].Conclusion Acupuncture on conception vessel,governor vessel an d bladder channel can improve the ability of learning and memory of HIE rats.