CAJ | 학술논문

目的观察活血通络方对缺血性肾病早期大鼠的肾组织及其单核细胞趋化蛋白1(MCP-1)表达的影响,探讨其防治缺血性肾病早期病变的机制.方法采用双侧肾动脉不全结扎术导致的双侧肾动脉狭窄建立缺血性肾病动物模型.将60只SD雄性大鼠按随机数字表法随机分为假手术组、模型组、预防组、药物组,每组各15只,模型组、药物组及预防组均行双侧肾动脉不全结扎术,假手术组仅分离双侧肾动脉但不结扎.假手术组(术后次日灌胃0.9％氯化钠注射液3ml/只,1次/d,共灌胃21d)、模型组(术后次日灌胃0.9％氯化钠注射液3 ml/只,1次/d,共灌胃21 d)、预防组[术前3d起灌胃活血通络方10.85g/(kg·d),稀释成3 ml/只,用量为成人用量单位体质量的7倍,1次/d,共灌胃24 d]、药物组[术后次日起灌胃活血通络方10.85 g/(kg·d),稀释成3 ml/只,用量为成人用量单位体质量的7倍,1次/d,共灌胃21 d]采用苏木精-伊红(HE)染色及Masson染色,光镜下观察肾组织病理学变化,并对各组进行病理评分比较;应用免疫组织化学检测各组大鼠肾组织中MCP-1的表达.结果 HE染色及Masson染色结果显示模型组、预防组、药物组大鼠肾组织与假手术组比较都有不同程度的炎症及纤维化病灶,预防组、药物组与模型组比较,肾小球硬化指数、肾小管间质损伤程度和肾组织MCP-1表达均明显降低,分别为(0.39±0.18)％、(0.83±0.12)％比(1.88±0.21)％;(1.21±0.12)分、(1.71±1.29)分比(2.65±1.23)分;313 ±8、483±12比743±9,均P＜0.01,且预防组结果优于药物组(P＜0.01).结论活血通络方可能通过减少MCP-1的表达,从而防治缺血性肾病的早期病变.
목적 관찰활혈통락방대결혈성신병조기대서적신조직급기단핵세포추화단백1(MCP-1)표체적영향,탐토기방치결혈성신병조기병변적궤제.방법 채용쌍측신동맥불전결찰술도치적쌍측신동맥협착건립결혈성신병동물모형.장60지SD웅성대서안수궤수자표법수궤분위가수술조、모형조、예방조、약물조,매조각15지,모형조、약물조급예방조균행쌍측신동맥불전결찰술,가수술조부분리쌍측신동맥단불결찰.가수술조(술후차일관위0.9％록화납주사액3ml/지,1차/d,공관위21d)、모형조(술후차일관위0.9％록화납주사액3 ml/지,1차/d,공관위21 d)、예방조[술전3d기관위활혈통락방10.85g/(kg·d),희석성3 ml/지,용량위성인용량단위체질량적7배,1차/d,공관위24 d]、약물조[술후차일기관위활혈통락방10.85 g/(kg·d),희석성3 ml/지,용량위성인용량단위체질량적7배,1차/d,공관위21 d]채용소목정-이홍(HE)염색급Masson염색,광경하관찰신조직병이학변화,병대각조진행병리평분비교;응용면역조직화학검측각조대서신조직중MCP-1적표체.결과 HE염색급Masson염색결과현시모형조、예방조、약물조대서신조직여가수술조비교도유불동정도적염증급섬유화병조,예방조、약물조여모형조비교,신소구경화지수、신소관간질손상정도화신조직MCP-1표체균명현강저,분별위(0.39±0.18)％、(0.83±0.12)％비(1.88±0.21)％;(1.21±0.12)분、(1.71±1.29)분비(2.65±1.23)분;313 ±8、483±12비743±9,균P＜0.01,차예방조결과우우약물조(P＜0.01).결론 활혈통락방가능통과감소MCP-1적표체,종이방치결혈성신병적조기병변.
Objective To observe the impact of Huoxue Tongluo Formula on renal tissue and monocyte chemotactic protein 1 (MCP-1) expression in the early ischemic nephropathy rats and explore the mechanism of prevention and treatment of early ischemic nephropathy.Methods The animal model of ischemic nephropathy was established using bilateral renal arterial stenosis induced by incomplete ligation of bilateral renal arteries.60 SD male rats were randomized into a sham-operation group,a model group,a prevention group and a medication group with 15 rats in each group.The model group,the prevention group and the medication group all took the Incomplete ligation of bilateral renal artery.The sham-operation group just had the renal artery separated,but did not have the operation.The prevention group was given the Huoxue Tongluo Formula by gavage 3 days before operation[10.85 g/ (kg · d),3 ml for each rat] ; the medication group was given the equivalent medicine on the second day after operation; the sham-operation group and model group were given 0.9％ sodium chloride injection (3 ml each rat).The materials were collected within 21 days after modeling.The pathological changes in renal tissues were observed.The MCP-1 expression in renal tissue was detected by immunohistochemisty.Results HE staining and Masson staining showed the inflammatory disorders and fibrosis foci had more changes in the model group,the prevention group,the medication group than in the sham-operation group.Compared with the model group,glomerular sclerosis index (CSI) 、tubule interstitial score(T IS) and the MCP-1 expression were reduced significantly in the prevention group and the medication group[(0.39 ±0.18)％,(0.83 ±0.12)％ vs (1.88 ±0.21)％ ;(1.21 ±0.12)score,(1.71 ± 1.29) score vs (2.65 ± 1.23) score,313 ±8,483 ± 12 vs 743 ±9,all P＜0.01].Conclusion Huoxue Tongluo Formula can prevent and treat early ischemic nephropathy via reducing the expression of MCP-1.