CAJ | 학술논문

目的分析雷帕霉素靶蛋白(mTOR)、70 ku核糖体蛋白S6激酶(P70S6K)的表达水平与胃癌常见临床病理因素的关系.方法采用逆转录聚合酶链反应技术,蛋白质印迹技术(Western-blot)检测32例胃癌患者癌组织、癌旁胃组织以及22例非胃癌患者胃组织中磷酸化雷帕霉素靶蛋白(mTOR)及磷酸化70 ku核酸体蛋白S6激酶(P70S6)K mRNA和蛋白水平的表达情况.结果 p-mTOR mRNA在胃癌组织中的表达水平(0.61±0.11)明显高于癌旁胃组织(0.43±0.10)及非胃癌胃组织(0.39 ±0.07),p-P70S6KmRNA在胃癌组织中的表达水平(0.63 ±0.13)亦明显高于癌旁胃组织(0.45 ±0.14)及非胃癌胃组织(0.44±0.15),差异均有统计学意义(均P＜0.05);mTOR mRNA和P70S6K mRNA在胃癌组织中的表达呈正相关(r=0.521,P=0.033);Western-blot检测结果也证实p-mTOR及p-P70S6K在胃癌组织中的表达高于癌旁胃组织及非胃癌胃组织;mTOR及P70S6K mRNA在胃癌组织中的表达水平与病理分期、淋巴结转移等明显相关,而与肿瘤直径、性别等无明显关系.结论 mTOR/P70S6K信号通路在胃癌组织中过度表达可能是胃癌发生的重要机制之一.
목적 분석뢰파매소파단백(mTOR)、70 ku핵당체단백S6격매(P70S6K)적표체수평여위암상견림상병리인소적관계.방법 채용역전록취합매련반응기술,단백질인적기술(Western-blot)검측32례위암환자암조직、암방위조직이급22례비위암환자위조직중린산화뢰파매소파단백(mTOR)급린산화70 ku핵산체단백S6격매(P70S6)K mRNA화단백수평적표체정황.결과 p-mTOR mRNA재위암조직중적표체수평(0.61±0.11)명현고우암방위조직(0.43±0.10)급비위암위조직(0.39 ±0.07),p-P70S6KmRNA재위암조직중적표체수평(0.63 ±0.13)역명현고우암방위조직(0.45 ±0.14)급비위암위조직(0.44±0.15),차이균유통계학의의(균P＜0.05);mTOR mRNA화P70S6K mRNA재위암조직중적표체정정상관(r=0.521,P=0.033);Western-blot검측결과야증실p-mTOR급p-P70S6K재위암조직중적표체고우암방위조직급비위암위조직;mTOR급P70S6K mRNA재위암조직중적표체수평여병리분기、림파결전이등명현상관,이여종류직경、성별등무명현관계.결론 mTOR/P70S6K신호통로재위암조직중과도표체가능시위암발생적중요궤제지일.
Objective To evaluate the mammalian target of rapamycin(mTOR)/70 ku ribosomal protein S6 kinase(P70S6K)in gastric carcinoma.Methods Reverse transcription-polymerase chain reaction and Western-blot were used to determine the expression of mTOR/P70S6K signaling pathway in cancerous and paracancerous tissues in 32 cases and 22 normal healthy tissues.Results The expression level of mTOR mRNA was markedly higher in the gastric carcinoma tissues(0.61 ± 0.11) than in the paracancerous tissues(0.43 ± 0.10) and normal stomach tissues(0.39 ± 0.07) ; the expression of P70S6K mRNA was also higher in the gastric carcinoma tissues (0.63 ± 0.13) than in the paracancerous tissues (0.45 ± 0.14) and normal stomach tissues (0.44 ±0.15),with a significant difference among groups (P ＜0.05).There was a positive correlation between mTOR mRNA and P70S6K mRNA expression in gastric carcinoma tissues(r =0.521,P =0.033).Compared to those in other two groups,the protein expression of mTOR,P70S6K in gastric carcinoma were significantly increased.The expression of mTOR/P70S6K signaling pathway in the gastric carcinom tissues was significantly correlated with the pathological grading and lymph node metastasis,but not with the diameter and gender.Conclusions The mTOR/P70S6K signaling pathway is specifically activated in gastric carcinom tissues.The mTOR/P70S6K signaling pathway might play an important role in the pathogenesis of gastric carcinoma.