CAJ | 학술논문

目的检测基质细胞衍生因子-1(SDF-1)及其受体CXCR4在阴道念珠菌病小鼠模型阴道组织及腰引流淋巴结中的表达情况.方法采用雌激素化阴道念珠菌病小鼠模型,雌性昆明小鼠(80只)随机分四组,A组(雌激素处理感染组)、B组(雌激素处理未感染组)、C组(未用雌激素处理感染组)、正常对照组(5只).前三组又再分为接种白念珠菌后第2、4、7、14和21天组(各5只).接种后不同时间点随机取小鼠阴道组织及腰淋巴结,以RT-PCR及免疫组化SABC法检测SDF-1及CXCR4 mRNA及蛋白水平的表达.结果 (1)RT-PCR:与正常小鼠相比,雌激素(B组)对SDF-1及CXCR4 mRNA表达无影响.①小鼠阴道组织:与B组相比,A组小鼠接种后各时间点均见SDF-1 mRNA表达明显增加(F=114.87,P＜0.01),第14天CXCR4 mRNA明显增加(t=15.61,P＜0.01);与正常小鼠相比,C组小鼠接种后第2,4,7天,SDF-1 mRNA明显增加;第4～21天CXCR4 mRNA均明显增加.②腰淋巴结:与B组相比,A组小鼠接种后第2天和7～21天SDF-1 mRNA明显增加(F=865.62,P＜0.01),CXCR4 mRNA于各时间点表达均增加(F=161.30,P＜0.01);与正常小鼠相比,C组小鼠接种后SDF-1 mRNA表达无明显改变(P＞0.05);CXCR4 mRNA于第7天和14天明显增加(P＜0.05).(2)免疫组化:A组小鼠阴道黏膜中SDF-1及CXCR4表达在接种后第2天即开始增加,第7天时达高峰,第14天和21天开始卜降,但均明显高于对照组(P＜0.05).结论感染组小鼠阴道黏膜及腰淋巴结中SDF-1及CXCR4的mRNA及蛋白水平均明显升高.
목적 검측기질세포연생인자-1(SDF-1)급기수체CXCR4재음도념주균병소서모형음도조직급요인류림파결중적표체정황.방법 채용자격소화음도념주균병소서모형,자성곤명소서(80지)수궤분사조,A조(자격소처리감염조)、B조(자격소처리미감염조)、C조(미용자격소처리감염조)、정상대조조(5지).전삼조우재분위접충백념주균후제2、4、7、14화21천조(각5지).접충후불동시간점수궤취소서음도조직급요림파결,이RT-PCR급면역조화SABC법검측SDF-1급CXCR4 mRNA급단백수평적표체.결과 (1)RT-PCR:여정상소서상비,자격소(B조)대SDF-1급CXCR4 mRNA표체무영향.①소서음도조직:여B조상비,A조소서접충후각시간점균견SDF-1 mRNA표체명현증가(F=114.87,P＜0.01),제14천CXCR4 mRNA명현증가(t=15.61,P＜0.01);여정상소서상비,C조소서접충후제2,4,7천,SDF-1 mRNA명현증가;제4～21천CXCR4 mRNA균명현증가.②요림파결:여B조상비,A조소서접충후제2천화7～21천SDF-1 mRNA명현증가(F=865.62,P＜0.01),CXCR4 mRNA우각시간점표체균증가(F=161.30,P＜0.01);여정상소서상비,C조소서접충후SDF-1 mRNA표체무명현개변(P＞0.05);CXCR4 mRNA우제7천화14천명현증가(P＜0.05).(2)면역조화:A조소서음도점막중SDF-1급CXCR4표체재접충후제2천즉개시증가,제7천시체고봉,제14천화21천개시복강,단균명현고우대조조(P＜0.05).결론 감염조소서음도점막급요림파결중SDF-1급CXCR4적mRNA급단백수평균명현승고.
Objective To examine the expressions of stroma-derived factor 1(SDF-1)and its receptor CXCR4 in murine vaginal tissue and lumbar lymph nodes during an experimental Candida albicans vaginal infection.Methods A murine model of vaginal candidiasis was established.Eighty female mice were randomly divided into 4 groups,25 in group A (estrogen-treated mice inoculated with referrence C.albicans strain),25 in group B(estrogen-treated mice inoculated with phosphate buffered solution),25 in group C(untreated mice infectcd with C albicalls)and 5 untreated mice free of infection in the control group.The first 3 groups were equally divided into 5 subgroups,including 2-,4-,7-,14-and 21-day groups.The mRNA and protein expression of SDF-1 and CXCR4 in the murine vaginal tissue and draining lumbar lymph nodes was assessed by RT-PCR and immunohistochemistry,respectively,2,4,7,14 and 21 days after the inoculation of C albicans.Results No significant differcnce was observed in the mRNA expression of SDF-1 or CXCR4 between group B and the control group.Compared with group B,the mRNA level of SDF-1 in group A increased both in vaginal tissue from day 2 to 21(F=114.87,P＜0.01)and in lumbar lymph nodes on dav 2,7,14 and 21:increased mRNA expression of CXCR4 was observed in vaginal tissue on day 14(t=15.61,P＜0.01)and lumbar lymph nodes from day 2 to 21(F=161.30,P＜0.01)in group A.In group C,the mRNA expression of SDF-1 significantly inereased in vaginal tissue on day 2,4 and 7 but remained unchanged in lumbar lymph tissues through the test period(P＞0.05),and an significant increase was seen in mRNA expression of CXCR4 in vaginal tissue from day 4 to 21 and in lumbar lymph tissues on dav 7 and 14 in comparison with the control group(all P＜0.05).As immunohistochemistry showed,the expression levels Of SDF-1 and CXCR4 in vaginal tissue of group A commenced to increase on day 2,reached a peak on day 7,then decreased on day 14 and 21 following the infeciton(P＜0.05),but they were consistently higher than those of the control group through the study period.Conclusion An increase in mRNA and protein expressions of SDF-1 and CXCR4 is noticed in vaginal tissue and lumbar lymph nodes of C.albicans-infected mice.