中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
1期
23-25
,共3页
李妍%陈喜雪%赵俊郁%汪科%钟珊%晋红中%闫言%陈金波%徐浩翔%曾跃平%吴圣贤%朱学骏
李妍%陳喜雪%趙俊鬱%汪科%鐘珊%晉紅中%閆言%陳金波%徐浩翔%曾躍平%吳聖賢%硃學駿
리연%진희설%조준욱%왕과%종산%진홍중%염언%진금파%서호상%증약평%오골현%주학준
类天疱疮,大疱性%免疫学试验%诊断%BP180
類天皰瘡,大皰性%免疫學試驗%診斷%BP180
류천포창,대포성%면역학시험%진단%BP180
Pemphigoid,bullous%Immunologic tests%Diagnosis%BP180
目的 探讨BP180抗体诊断试剂盒检测大疱性类天疱疮(BP)的灵敏度和特异度.方法 多中心随机双盲平行对照临床试验.使用BP180抗体诊断试剂盒(ELISA)检测106例临床已经确诊的活动期BP患者和106例对照人群(含非BP的其他大疱性疾病以及硬皮病、银屑病、SLE、妊娠晚期孕妇、健康献血员等)血清样本,并与间接免疫荧光(IIF)试验进行灵敏度和特异度的对比.结果 106份BP患者血清样本中81份BP180抗体诊断试剂盒为阳性,其灵敏度为76.4%;83份抗表皮抗原抗体检测试剂盒(IIF法)为阳性,其灵敏度为78.3%.106份对照组血清标本中95份BP180抗体诊断试剂盒为阴性,其特异度为89.6%;102份抗表皮抗原抗体检测试剂盒为阴性,其特异度为96.2%.BP180抗体诊断试剂盒(ELISA)与对照试剂盒的灵敏度和特异度相比较,差异无统计学意义(P＞0.05).结论 BP180抗体诊断试剂盒可作为诊断BP的一种辅助手段.
目的 探討BP180抗體診斷試劑盒檢測大皰性類天皰瘡(BP)的靈敏度和特異度.方法 多中心隨機雙盲平行對照臨床試驗.使用BP180抗體診斷試劑盒(ELISA)檢測106例臨床已經確診的活動期BP患者和106例對照人群(含非BP的其他大皰性疾病以及硬皮病、銀屑病、SLE、妊娠晚期孕婦、健康獻血員等)血清樣本,併與間接免疫熒光(IIF)試驗進行靈敏度和特異度的對比.結果 106份BP患者血清樣本中81份BP180抗體診斷試劑盒為暘性,其靈敏度為76.4%;83份抗錶皮抗原抗體檢測試劑盒(IIF法)為暘性,其靈敏度為78.3%.106份對照組血清標本中95份BP180抗體診斷試劑盒為陰性,其特異度為89.6%;102份抗錶皮抗原抗體檢測試劑盒為陰性,其特異度為96.2%.BP180抗體診斷試劑盒(ELISA)與對照試劑盒的靈敏度和特異度相比較,差異無統計學意義(P＞0.05).結論 BP180抗體診斷試劑盒可作為診斷BP的一種輔助手段.
목적 탐토BP180항체진단시제합검측대포성류천포창(BP)적령민도화특이도.방법 다중심수궤쌍맹평행대조림상시험.사용BP180항체진단시제합(ELISA)검측106례림상이경학진적활동기BP환자화106례대조인군(함비BP적기타대포성질병이급경피병、은설병、SLE、임신만기잉부、건강헌혈원등)혈청양본,병여간접면역형광(IIF)시험진행령민도화특이도적대비.결과 106빈BP환자혈청양본중81빈BP180항체진단시제합위양성,기령민도위76.4%;83빈항표피항원항체검측시제합(IIF법)위양성,기령민도위78.3%.106빈대조조혈청표본중95빈BP180항체진단시제합위음성,기특이도위89.6%;102빈항표피항원항체검측시제합위음성,기특이도위96.2%.BP180항체진단시제합(ELISA)여대조시제합적령민도화특이도상비교,차이무통계학의의(P＞0.05).결론 BP180항체진단시제합가작위진단BP적일충보조수단.
Objective To evaluate the sensitivity and specificity of BP180NC16a-ELISA in the diagnosis of bullous pemphigoid (BP). Methods A multi-center, randomized, double-blind, parallel-controlled study was conducted. Sera were collected from 106 patients with clinically confirmed active BP and 106 control subjects including patients with non-BP bullous diseases, scleroderma, psoriasis or systemic lupus erythematosus,late pregnant women and healthy blood donors. BP180NC16a-ELISA was performed on these sera. The IgG antibody levels measured by ELISA kit were compared with those measured by indirect immunofluorescence (IIF) test. Results Of the 106 BP sera, 81 were positive for BP180NC16a-ELISA with a sensitivity of 76.4%,83 for ⅡF test with a sensitivity of 78.3%. Among the 106 control serum samples, 95 were negative for BP180NC16A-ELISA with a specificity of 89.6%, and 102 for ⅡF test with a specificity of 96.2%. There was no significant difference between the two tests in dignostic sensitivity and specificity for BP (both P ＞ 0.05).Conclusion BP180NC16A-ELISA may serve as an adjuvant tool for the diagnosis of BP.
