中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
8期
578-580
,共3页
蓝艳%蒋远文%唐秀生%武洁
藍豔%蔣遠文%唐秀生%武潔
람염%장원문%당수생%무길
红斑狼疮,系统性%白细胞介素17%多态性,单核苷酸
紅斑狼瘡,繫統性%白細胞介素17%多態性,單覈苷痠
홍반랑창,계통성%백세포개소17%다태성,단핵감산
Lupus erythematosus,systemic%Interleukin-17%Polymorphism,single nucleotide
目的 探讨白介素27(IL-27)基因单核苷酸多态性与广西壮族系统性红斑狼疮(SLE)易感性之间的关系.方法 以135例SLE患者和150例正常人对照者为研究对象,应用聚合酶链反应-限制性片段长度多态性和DNA测序的方法对IL-27基因-964 A/G、2905 T/G单核苷酸多态性进行基因分型.结果 SLE组和正常人对照组中IL-27基因2905 T/G多态性分布差异无统计学意义(x2=1.63,P＞0.05),而IL-27基因-964 A/G多态性的分布差异有统计学意义(x2=9.88,P＜0.01).等位基因频率的相对风险分析发现,-964 G等位基因携带者患SLE的风险是-964 A等位基因的1.725倍(OR=1.725,95%CI:1.227～2.425).联合基因型分析发现,IL-27的-964 G/2905 G等位基因频率SLE组(10.7%)显著高于对照组(5.3%)(P＜0.01),-964 G/2905 G等位基因携带者显著增加了SLE的发病风险(OR=2.351,95% CI:1.228～4.501).结论 IL-27基因-964 A/G多态性与SLE的发病具有相关性,其中-964G等位基因可能是SLE的遗传易感基因.
目的 探討白介素27(IL-27)基因單覈苷痠多態性與廣西壯族繫統性紅斑狼瘡(SLE)易感性之間的關繫.方法 以135例SLE患者和150例正常人對照者為研究對象,應用聚閤酶鏈反應-限製性片段長度多態性和DNA測序的方法對IL-27基因-964 A/G、2905 T/G單覈苷痠多態性進行基因分型.結果 SLE組和正常人對照組中IL-27基因2905 T/G多態性分佈差異無統計學意義(x2=1.63,P＞0.05),而IL-27基因-964 A/G多態性的分佈差異有統計學意義(x2=9.88,P＜0.01).等位基因頻率的相對風險分析髮現,-964 G等位基因攜帶者患SLE的風險是-964 A等位基因的1.725倍(OR=1.725,95%CI:1.227～2.425).聯閤基因型分析髮現,IL-27的-964 G/2905 G等位基因頻率SLE組(10.7%)顯著高于對照組(5.3%)(P＜0.01),-964 G/2905 G等位基因攜帶者顯著增加瞭SLE的髮病風險(OR=2.351,95% CI:1.228～4.501).結論 IL-27基因-964 A/G多態性與SLE的髮病具有相關性,其中-964G等位基因可能是SLE的遺傳易感基因.
목적 탐토백개소27(IL-27)기인단핵감산다태성여엄서장족계통성홍반랑창(SLE)역감성지간적관계.방법 이135례SLE환자화150례정상인대조자위연구대상,응용취합매련반응-한제성편단장도다태성화DNA측서적방법대IL-27기인-964 A/G、2905 T/G단핵감산다태성진행기인분형.결과 SLE조화정상인대조조중IL-27기인2905 T/G다태성분포차이무통계학의의(x2=1.63,P＞0.05),이IL-27기인-964 A/G다태성적분포차이유통계학의의(x2=9.88,P＜0.01).등위기인빈솔적상대풍험분석발현,-964 G등위기인휴대자환SLE적풍험시-964 A등위기인적1.725배(OR=1.725,95%CI:1.227～2.425).연합기인형분석발현,IL-27적-964 G/2905 G등위기인빈솔SLE조(10.7%)현저고우대조조(5.3%)(P＜0.01),-964 G/2905 G등위기인휴대자현저증가료SLE적발병풍험(OR=2.351,95% CI:1.228～4.501).결론 IL-27기인-964 A/G다태성여SLE적발병구유상관성,기중-964G등위기인가능시SLE적유전역감기인.
Objective To investigate the association between the single nucleotide polymorphisms of interleukin-27 (IL-27) gene and susceptibility to systematic lupus erythematosus (SLE) in Guangxi Zhuang population. Methods In total, 135 patients with SLE and 150 age- and sex-matched human controls of Zhuang nationality were recruited in this study. PCR-restriction fragment length polymorphism (RFLP) analysis and DNA sequencing were performed to analyze the IL-27 gene -964 A/G and -2905 T/G polymorphisms.Results Significant differences were observed in the distribution of IL-27 gene -964 A/G polymorphism (x2 =9.88, P ＜ 0.01 ). The relative risk for SLE in carriers of G allele at position 964 of IL-27 gene was 1.725 times that in carriers of A allele at this position (OR = 1.725,95% CI: 1.227 - 2.425). A significant increase was observed in the frequency of 964G/2905G alleles of IL-27 gene in patients with SLE compared with the controls (10.7% vs. 5.3%, P ＜ 0.01 ), and the 964G/2905G alleles were associated with a significantly increased risk for SLE (OR = 2.351, 95% CI: 1.228 - 4.501 ). Conclusions The IL-27 gene -964 A/G polymorphism is associated with the development of SLE, and the -964 G allele may increase the genetic susceptibility to SLE.
