中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
11期
761-764
,共4页
鞠强%余茜%宋宁静%谈亦%夏隆庆%Christos C. Zouboulis
鞠彊%餘茜%宋寧靜%談亦%夏隆慶%Christos C. Zouboulis
국강%여천%송저정%담역%하륭경%Christos C. Zouboulis
受体%芳基烃%氯痤疮%二恶辛类%表皮%皮脂腺%毛囊%细胞系
受體%芳基烴%氯痤瘡%二噁辛類%錶皮%皮脂腺%毛囊%細胞繫
수체%방기경%록좌창%이악신류%표피%피지선%모낭%세포계
follicle%Cell line
目的 观察芳香烃受体(AhR)在人表皮、毛囊及皮脂腺细胞上的表达及在二(噁)英作用下的激活情况.方法 采用免疫荧光和免疫组化法观察AhR蛋白在人面部表皮、毛囊、皮脂腺及体外培养的HaCaT细胞和SZ95人皮脂腺细胞上的表达情况；Western印记法观察2,3,7,8-四氯二苯-p-二(噁)英(TCDD)作用HaCaT细胞和SZ95细胞3d后AhR蛋白表达的变化情况.结果 AhR蛋白在人面部皮肤表皮、毛囊、皮脂腺细胞上表达,并且AhR蛋白在表皮棘层和颗粒层的表达强于基底细胞层,在皮脂腺小叶的外周和中央表达强于皮脂腺小叶中间,在毛囊外毛根鞘和近毛小皮细胞上的表达强于内毛根鞘细胞.AhR蛋白在HaCaT细胞和SZ95细胞的细胞核和胞质中高表达.在TCDD作用下,HaCaT细胞和SZ95细胞内AhR蛋白表达明显下降,显示AhR在TCDD作用下被激活.结论 AhR在人表皮、毛囊、皮脂腺、HaCaT细胞及SZ95细胞上高表达,并在TCDD作用下激活,显示二(噁)英有可能通过AhR信号途径引起了人表皮、毛囊和皮脂腺的异常分化.
目的 觀察芳香烴受體(AhR)在人錶皮、毛囊及皮脂腺細胞上的錶達及在二(噁)英作用下的激活情況.方法 採用免疫熒光和免疫組化法觀察AhR蛋白在人麵部錶皮、毛囊、皮脂腺及體外培養的HaCaT細胞和SZ95人皮脂腺細胞上的錶達情況；Western印記法觀察2,3,7,8-四氯二苯-p-二(噁)英(TCDD)作用HaCaT細胞和SZ95細胞3d後AhR蛋白錶達的變化情況.結果 AhR蛋白在人麵部皮膚錶皮、毛囊、皮脂腺細胞上錶達,併且AhR蛋白在錶皮棘層和顆粒層的錶達彊于基底細胞層,在皮脂腺小葉的外週和中央錶達彊于皮脂腺小葉中間,在毛囊外毛根鞘和近毛小皮細胞上的錶達彊于內毛根鞘細胞.AhR蛋白在HaCaT細胞和SZ95細胞的細胞覈和胞質中高錶達.在TCDD作用下,HaCaT細胞和SZ95細胞內AhR蛋白錶達明顯下降,顯示AhR在TCDD作用下被激活.結論 AhR在人錶皮、毛囊、皮脂腺、HaCaT細胞及SZ95細胞上高錶達,併在TCDD作用下激活,顯示二(噁)英有可能通過AhR信號途徑引起瞭人錶皮、毛囊和皮脂腺的異常分化.
목적 관찰방향경수체(AhR)재인표피、모낭급피지선세포상적표체급재이(오)영작용하적격활정황.방법 채용면역형광화면역조화법관찰AhR단백재인면부표피、모낭、피지선급체외배양적HaCaT세포화SZ95인피지선세포상적표체정황；Western인기법관찰2,3,7,8-사록이분-p-이(오)영(TCDD)작용HaCaT세포화SZ95세포3d후AhR단백표체적변화정황.결과 AhR단백재인면부피부표피、모낭、피지선세포상표체,병차AhR단백재표피극층화과립층적표체강우기저세포층,재피지선소협적외주화중앙표체강우피지선소협중간,재모낭외모근초화근모소피세포상적표체강우내모근초세포.AhR단백재HaCaT세포화SZ95세포적세포핵화포질중고표체.재TCDD작용하,HaCaT세포화SZ95세포내AhR단백표체명현하강,현시AhR재TCDD작용하피격활.결론 AhR재인표피、모낭、피지선、HaCaT세포급SZ95세포상고표체,병재TCDD작용하격활,현시이(오)영유가능통과AhR신호도경인기료인표피、모낭화피지선적이상분화.
Objective To observe the expression of aryl hydrocarbon receptor (AhR) in human epidermis,hair follicles and sebaceous glands and the activation of AhR by dioxin.Methods Full-thickness skin specimens were obtained from 3 volunteers receiving cosmetic facial surgery.Indirect immunofluorescence and immunohistochemistry were used to detect the expression of AhR in these skin specimens,as well as on cultured human HaCaT keratinocytes and SZ95 sebocytes.Western blot was performed to detect the expressions of AhR protein on HaCaT cells and SZ95 sebocytes after 3-day treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Results AhR protein was found to be expressed in human epidermis,hair follicles and sebaceous glands,and the expression was stronger in the stratum spinosum and stratum granulosum than in the basal layer,in the outer and central layer than in the middle layer of sebaceous lobuli,in hair follicle outer root sheath and hair cuticles than in the inner root sheath.There was a high expression of AhR in the nucleus and cytoplasm of HaCaT and SZ95 cells.A significant decrease was observed in the expression of AhR in both HaCaT cells and SZ95 cells after treatment with TCDD for 3 days,hinting that AhR was activated by TCDD.Conclusions AhR is highly expressed in human epidermis,hair follicles and sebaceous glands,and can be activated by TCDD,suggesting that dioxin may induce the abnormal differentiation of epidermis,hair follicles and sebaceous glands via AhR singaling pathway.
