CAJ | 학술논문

目的检测PTEN和p-Akt在Kaposi肉瘤组织中的表达,初步探讨PTEN和p-Akt蛋白在Kaposi肉瘤发病中的作用及意义.方法应用免疫组织化学Envision二步法检测17例Kaposi肉瘤患者肿瘤组织及17例健康人正常皮肤组织内PTEN和p-Akt的表达水平.结果 PTEN、p-Akt蛋白主要表达于胞质.PTEN在Kaposi肉瘤组织中阳性表达率为76.5％(13/17),在正常皮肤组织中为88.2％(15/17),两组表达率差异无统计学意义(x2=2.65,P＞ 0.10).p-Akt在Kaposi肉瘤组织中阳性表达率为100％( 17/17),在正常皮肤组织中均呈阴性表达.结论 p-Akt蛋白的异常表达在Kaposi肉瘤的发展过程中可能发挥了一定作用.
목적 검측PTEN화p-Akt재Kaposi육류조직중적표체,초보탐토PTEN화p-Akt단백재Kaposi육류발병중적작용급의의.방법 응용면역조직화학Envision이보법검측17례Kaposi육류환자종류조직급17례건강인정상피부조직내PTEN화p-Akt적표체수평.결과 PTEN、p-Akt단백주요표체우포질.PTEN재Kaposi육류조직중양성표체솔위76.5％(13/17),재정상피부조직중위88.2％(15/17),량조표체솔차이무통계학의의(x2=2.65,P＞ 0.10).p-Akt재Kaposi육류조직중양성표체솔위100％( 17/17),재정상피부조직중균정음성표체.결론 p-Akt단백적이상표체재Kaposi육류적발전과정중가능발휘료일정작용.
Objective To investigate the protein expressions of PTEN and p-Akt in Kaposi's sarcoma (KS) tissue and their significance in the development of KS.Methods The EnVision two-step method was used to detect the expressions of PTEN and p-Akt proteins in tissue specimens from the lesions of 17 patients with KS and from the normal skin of 17 normal human controls.Results PTEN and P-Akt were mainly expressed in the cytoplasm of cells.No significant difference was observed in the positivity rate of PTEN [76.5％(13/17)vs.88.2％(15/17),P＞ 0.10] between the KS and control specimens.p-Akt was expressed in all (100％) of the 17 KS specimens,but in none of the control specimens.Conclusion The abnormal expression of PTEN protein may play a certain role in the development of KS.