中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
10期
714-717
,共4页
薛燕宁%陈浩%周武庆%曹玉萍%陶玥%曾学思%孙建方
薛燕寧%陳浩%週武慶%曹玉萍%陶玥%曾學思%孫建方
설연저%진호%주무경%조옥평%도모%증학사%손건방
黑色素瘤%胰岛素样生长因子结合蛋白质7%DNA甲基化
黑色素瘤%胰島素樣生長因子結閤蛋白質7%DNA甲基化
흑색소류%이도소양생장인자결합단백질7%DNA갑기화
Melanoma%Insulin-like growth factor binding protein 7%DNA methylation
目的 探讨人黑素瘤细胞株和原代黑素细胞IGFBP7启动子区CpG岛甲基化状态与IGFBP7表达的关系.方法 亚硫酸氢钠修饰后测序法检测4株人黑素瘤细胞和原代黑素细胞中IGFBP7基因启动子区CpG岛DNA甲基化状态.结果 IGFBP7表达阴性的3个细胞株(A375、M14、SK-MEL-1)和表达阳性的2个细胞株(MV3及原代黑素细胞)的甲基化模式存在差异,聚类分析各自聚为一类.结论 IGFBP7基因启动子区CpG岛甲基化状态和基因表达有一定相关性.
目的 探討人黑素瘤細胞株和原代黑素細胞IGFBP7啟動子區CpG島甲基化狀態與IGFBP7錶達的關繫.方法 亞硫痠氫鈉脩飾後測序法檢測4株人黑素瘤細胞和原代黑素細胞中IGFBP7基因啟動子區CpG島DNA甲基化狀態.結果 IGFBP7錶達陰性的3箇細胞株(A375、M14、SK-MEL-1)和錶達暘性的2箇細胞株(MV3及原代黑素細胞)的甲基化模式存在差異,聚類分析各自聚為一類.結論 IGFBP7基因啟動子區CpG島甲基化狀態和基因錶達有一定相關性.
목적 탐토인흑소류세포주화원대흑소세포IGFBP7계동자구CpG도갑기화상태여IGFBP7표체적관계.방법 아류산경납수식후측서법검측4주인흑소류세포화원대흑소세포중IGFBP7기인계동자구CpG도DNA갑기화상태.결과 IGFBP7표체음성적3개세포주(A375、M14、SK-MEL-1)화표체양성적2개세포주(MV3급원대흑소세포)적갑기화모식존재차이,취류분석각자취위일류.결론 IGFBP7기인계동자구CpG도갑기화상태화기인표체유일정상관성.
Objective To assess the relationship of methylation status of CpG islands in the promoter region of insulin-like growth factor binding protein 7 (IGFBP7) gene with the expression of IGFBP7 gene in human melanoma cell lines and primary melanocytes.Methods Primary melanocytes from human forcskin tissue as well as 4 human melanoma cell lines,including A375,M14,SK-MEL-1 and MV3,were used in this study.Bisulfite sequencing PCR (BSP) was applied to detect the methylation status of 54 CpG sites in the 5'-flanking promoter region of IGFBP7 gene in all of the melanoma cell lines and primary melanocytes.Results As hierarchical cluster analysis showed,IGFBP7-positive cells (including A375,M14 and SK-MEL-1 ) differed significantly from IGFBP7-negative cells (including MV3 cells and primary melanocytes) in the methylation pattern of IGFBP7 gene promoter region.Conclusion The methylation status of CpG island in the promoter region of IGFBP7 gene may be associated with its expression in melanoma cell lines.