CAJ | 학술논문

目的探讨他克莫司对淋巴细胞分泌IL-6和sIL-2R及表达IL-6和sIL-2RmRNA的影响.方法酶联免疫吸附试验测定不同浓度他克莫司对人Jurkat淋巴瘤细胞产生IL-6和sIL-2R的影响,实时荧光定量PCR法分析他克莫司对淋巴瘤细胞IL-6 mRNA和sIL-2R mRNA表达的影响.结果 102～ 104 nmol/L他克莫司可以抑制Jurkat淋巴瘤细胞分泌IL-6、sIL-2R(P值均＜0.05),其中 103～ 104 nmol/L 他克莫司作用显著.102 nmol/L他克莫司可下调淋巴瘤细胞IL-6和sIL-2R mRNA的表达(P值均＜0.05).结论适当浓度他克莫司可抑制淋巴细胞分泌IL-6和sIL-2R,下调IL-6和sIL-2R mRNA的表达.
목적 탐토타극막사대림파세포분비IL-6화sIL-2R급표체IL-6화sIL-2RmRNA적영향.방법 매련면역흡부시험측정불동농도타극막사대인Jurkat림파류세포산생IL-6화sIL-2R적영향,실시형광정량PCR법분석타극막사대림파류세포IL-6 mRNA화sIL-2R mRNA표체적영향.결과 102～ 104 nmol/L타극막사가이억제Jurkat림파류세포분비IL-6、sIL-2R(P치균＜0.05),기중 103～ 104 nmol/L 타극막사작용현저.102 nmol/L타극막사가하조림파류세포IL-6화sIL-2R mRNA적표체(P치균＜0.05).결론 괄당농도타극막사가억제림파세포분비IL-6화sIL-2R,하조IL-6화sIL-2R mRNA적표체.
Objective To evaluate the effects of tacrolimus on the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes.Methods Jurkat human lymphoma cells were cultured and treated with tacrolimus of different concentrations.Enzyme linked immunosorbent assay was performed to determine the levels of IL-6 and sIL-2R in the supernatant of Jurkat cells at 48 hours after treatment with tacrolimus of 0,10,102,103 and 104 nmol/L,and real time reverse transcription PCR to measure the expression of IL-6 mRNA and sIL-2R mRNA of Jurkat cells at 48 hours after treatment with tacrolimus of 102 nmol/L.Results Tacrolimus of 102 - 104 nmol/L could suppress the secretion of IL-6 and sIL-2R from Jurkat cells (all P＜ 0.05),with a more marked suppressing effect achieved by the use of tacrolimus at 103 - 104 nmol/L.The expressions of IL-6 and sIL-2R mRNA from Jurkat cells were downregulated by tacrolimus of 102 nmol/L (both P ＜ 0.05).Conclusion Tacrolimus at certain concentrations could downregulate the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes.