CAJ | 학술논문

178例儿童脓疱疮皮损中金黄色葡萄球菌药敏及随机扩增多态性DNA分析
178례인동농포창피손중금황색포도구균약민급수궤확증다태성DNA분석
Drug susceptibility testing and randomly amplified polymorphic DNA analysis of Staphylococcus aureus from 178 children with impetigo

万方数据

中华皮肤科杂志中華皮膚科雜誌 중화피부과잡지
Chinese Journal of Dermatology
2012年 11期 767-770 ,共4页

卞彩云%路永红%周培媚%王敏%陈明%蒋存火%蔡琦%李煊%张大维%冉琴%徐海涛%陈前明%刘艳%吴波%王振远%陈娜伊%向丹黎%黄旭蕾%保勇%吕燕%尹亚菲

변채운%로영홍%주배미%왕민%진명%장존화%채기%리훤%장대유%염금%서해도%진전명%류염%오파%왕진원%진나이%향단려%황욱뢰%보용%려연%윤아비

脓疱疮%葡萄球菌,金黄色%微生物敏感性试验%随机扩增多态DNA技术膿皰瘡%葡萄毬菌,金黃色%微生物敏感性試驗%隨機擴增多態DNA技術
농포창%포도구균,금황색%미생물민감성시험%수궤확증다태DNA기술
Impetigo%Staphylococcus aureus%Microbial sensitivity tests%Randomly amplified polymorphic DNA technique

目的探讨儿童脓疱疮皮损中金黄色葡萄球菌的耐药情况,比较敏感株与耐药株的DNA指纹差异.方法对成都地区178例儿童脓疱疮患者皮损分泌物进行细菌培养,对培养出的162株金黄色葡萄球菌(简称金葡菌)进行21种抗生素的药物敏感试验,同时对162株金葡菌进行随机扩增多态性DNA分析.结果从脓疱疮患儿皮损中分离鉴定出病原菌180株,其中金葡菌162株,占90.00％.162株金葡菌中,148株为甲氧西林敏感金葡菌(MSSA),14株为耐甲氧西林金葡菌(MRSA).162株金葡菌进行21种抗生素体外药敏试验,敏感率前5位分别为米诺环素、替考拉宁、喹奴普汀、万古霉素、呋喃妥因.耐药率前5位分别为青霉素、红霉素、克林霉素、复方磺胺甲(恶)唑、四环素.未发现对夫西地酸、呋喃妥因、万古霉素、喹奴普汀、替考拉宁及米诺环素耐药.按DNA条带的大小和数量进行分型,共分为8种基因型,基因型Ⅲ最多占31.48％;基因型Ⅱ占26.54％;基因型Ⅵ占25.93％,这3种基因型占总数的65.43％(106/162).148株MSSA的基因型有8种,基因型Ⅲ占33.78％,基因型Ⅵ占26.35％,基因型Ⅱ占22.30％.14株MRSA的基因型只有3种,分别为基因型Ⅱ10株(71.43％),基因型Ⅵ3株(21.43％),基因型Ⅲ1株(7.14％),以基因型Ⅱ为主.结论成都儿童脓疱疮皮损中病原菌以金葡菌为主,对米诺环素、替考拉宁及喹奴普汀等高度敏感.其RAPD指纹共分为8型,以基因型Ⅲ最多.
목적 탐토인동농포창피손중금황색포도구균적내약정황,비교민감주여내약주적DNA지문차이.방법 대성도지구178례인동농포창환자피손분비물진행세균배양,대배양출적162주금황색포도구균(간칭금포균)진행21충항생소적약물민감시험,동시대162주금포균진행수궤확증다태성DNA분석.결과 종농포창환인피손중분리감정출병원균180주,기중금포균162주,점90.00％.162주금포균중,148주위갑양서림민감금포균(MSSA),14주위내갑양서림금포균(MRSA).162주금포균진행21충항생소체외약민시험,민감솔전5위분별위미낙배소、체고랍저、규노보정、만고매소、부남타인.내약솔전5위분별위청매소、홍매소、극림매소、복방광알갑(악)서、사배소.미발현대부서지산、부남타인、만고매소、규노 보정、체고랍저급미낙배소내약.안DNA조대적대소화수량진행분형,공분위8충기인형,기인형Ⅲ최다점31.48％;기인형Ⅱ점26.54％;기인형Ⅵ점25.93％,저3충기인형점총수적65.43％(106/162).148주MSSA적기인형유8충,기인형Ⅲ점33.78％,기인형Ⅵ점26.35％,기인형Ⅱ점22.30％.14주MRSA적기인형지유3충,분별위기인형Ⅱ10주(71.43％),기인형Ⅵ3주(21.43％),기인형Ⅲ1주(7.14％),이기인형Ⅱ위주.결론 성도인동농포창피손중병원균이금포균위주,대미낙배소、체고랍저급규노보정등고도민감.기RAPD지문공분위8형,이기인형Ⅲ최다.
Objective To test the antimicrobial susceptibility of Staphylococcus aureus from children with impetigo,and to assess the differences in randomly amplified polymorphic DNA profiles between sensitive and resistant Staphylococcus aureus strains.Methods Secretion specimens were obtained from the impetiginous lesions of 178 children,and subjected to bacterial culture.The susceptibility of 162 Staphylococcus aureus isolates against 21 antibiotics was tested.Randomly amplified polymorphic DNA PCR(RAPD-PCR)was performed to characterize the genotype of Staphylococcus aureus.Results Totally,180 bacterial strains were isolated from 178 children with impetigo in Chengdu,including 162(90.00％)Staphylococcus aureus strains.Of the 162 Staphylococcus aureus strains,148 were methicillin sensitive Staphylococcus aureus(MSSA),14 methicillin resistant Staphylococcus aureus(MRSA).The most active antibiotic was minocycline,followed by teicoplanin,quinupristin,vancomycin and nitrofurantoin,while the resistance rate to penicillin was highest,followed by that to erythromycin,clindamycin,compound sulfamethoxazole and tetracycline.All the Staphylococcus aureus isolates were sensitive to fusidic acid,nitrofurantoin,vancomycin,minocycline and teicoplanin.According to RAPD-PCR,the 162 Staphylococcus aureus strains were divided into 8 genotypes,with the three most prevalent genotypes being Ⅲ(31.48％),Ⅱ(26.54％)and Ⅵ(25.93％),which accounted for 65.43％(106/162)in all the strains.The 148 MSSA strains fell into 8 genotypes,with genotype Ⅲ(50 strains,33.78％),Ⅵ(39 strains,26.35％)and Ⅱ(33 strains,22.30％)being the most prevalent genotypes;the 14 MRSA strains fell into 3 genotypes,i.e.,genotype Ⅱ(10 strains,71.43％),Ⅵ(3 strains,21.43％),and Ⅲ(1 strain,7.14％).Conclusions Staphylococcus aureus is the most prevalent pathogenic bacteria in children with impetigo in Chengdu area,which is highly sensitive to minocycline,teicoplanin and quinupristin,and falls into 8 genotypes according to RAPD-PCR with genotype Ⅲ being the most common genotype.